2.1.1.202 additional information a catalytic model for the RNA m5C methyltransferases that utilizes both conserved cysteines. The TC-Cys is proposed to perform covalent catalysis while the PC-Cys plays an important role in product release Saccharomyces cerevisiae ? - ? 89 2.1.1.202 additional information Cys310 of motif VI is likely the nucleophilic catalyst of Trm4p Saccharomyces cerevisiae ? - ? 89 2.1.1.202 additional information highly conserved residues form a large, positively charged surface, which seems to be suitable for tRNA binding. Substrate specificity and methylation sites on tRNA, overview Methanocaldococcus jannaschii ? - ? 89 2.1.1.202 additional information identification of single-nucleotide resolution of cytosine 5-methylation sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview. The enzyme does not methylate cytosines at structural positions C47, C48, C49 and C72. Identification of modified cytosines in Arabidopsis thaliana nuclear transcribed tRNAs which are dependent on RMTases TRDMT1 and TRM4B. No cytosine 5-methylation sites are detected in Arabidopsis chloroplast or mitochondrial tRNAs, which is in contrast to animal mitochondrial tRNAs Arabidopsis thaliana ? - ? 89 2.1.1.202 additional information identification of single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview Brassica rapa ? - ? 89 2.1.1.202 additional information identification of single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview Triticum turgidum subsp. durum ? - ? 89 2.1.1.202 additional information identification of single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview Ginkgo biloba ? - ? 89 2.1.1.202 additional information identification of single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview Nannochloropsis oculata ? - ? 89 2.1.1.202 additional information identification of single-nucleotide resolution of m5C sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species, overview Caulerpa taxifolia ? - ? 89 2.1.1.202 additional information NSUN2 candidate ncRNA targets identified include RNAs with central functions in the processing, folding and modification of other ncRNAs (RPPH1, Y RNA, SCARNA2), RNAs important for protein synthesis and trafficking (5S rRNA and 7SL RNA) and RNAs involved in multidrug resistance and other processes (Vault RNAs). Notably, all of the NSUN2 targets revealed by 5-azacytidine-mediated RNA immunoprecipitation are either transcribed by RNA Pol III in the nucleolus (SCARNA2 excepted), or function in the nucleolus (SCARNA2), where NSUN2 resides Homo sapiens ? - ? 89 2.1.1.202 additional information the enzyme Trm4 fabricate 5-methylcytosine (m5C) in RNA molecules utilizing a dual-cysteine catalytic mechanism Saccharomyces cerevisiae ? - ? 89 2.1.1.202 additional information the iCLIP method identifies tRNA AspGTC, ValAAC, GlyGCC, and LeuCAA as methylation substrates with methylation within the variable arm at cytosines 48, 49, and 50, no additional NSun2 target sites outside the variable arm. vtRNAs are methylation substrates for NSun2, vtRNAs are ncRNAs found as part of the vault ribonucleoprotein complex Homo sapiens ? - ? 89 2.1.1.202 additional information development and evaluation of 5-azacytidine-mediated RNA immunoprecipitation, a mechanism-based technique in nine steps that exploits the covalent bond formed between an RNA methyltransferase and the cytidine analogue 5-azacytidine to recover RNA targets by immunoprecipitation, overview. The method enables over 200fold enrichment of tRNAs that are known targets of the enzyme revealing many tRNA and non-coding RNA targets not previously associated with NSUN2. High frequency of C>G transversions at the cytosine residues targeted by the enzyme, allowing identification of the specific methylated cytosine(s) in target RNAs. tRNAGly(GCC), bears four NSUN2 target sites (C40, 48, 49 and 50) Homo sapiens ? - ? 89 2.1.1.202 additional information development and evaluation of a customized version of the individual-nucleotide-resolution crosslinking and immunoprecipitation (iCLIP) method for detection of cytosine methylation in RNA species, site-specific methylation in tRNAs and additional messenger and noncoding RNAs (ncRNAs), overview. Identified NSun2 targets are tRNAs, mRNAs, and ncRNAs Homo sapiens ? - ? 89 2.1.1.202 additional information motif I is essential for methyltransferase activity and is required for S-adenosyl-L-methionine binding and catalysis. Enzyme variant Trm4a is lacking motif I in contrast to enzyme variant Trm4b Arabidopsis thaliana ? - ? 89 2.1.1.202 additional information the enzyme forms covalent complexes with previously methylated RNA requiring S-adenosyl-L-homocysteine, the removal of this metabolite results in the disassembly of preexisting complexes Saccharomyces cerevisiae ? - ? 89 2.1.1.202 additional information C34 methylation depends on Trm4a in both tRNALeuCAA and tRNAProCGG. Substrate specificity, comparison to trm4b, overview Schizosaccharomyces pombe ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN2, overview. NSUN2 has a much broader target spectrum and is able to modify several positions (C34, C40, C48, C49, and C50) in a number of different tRNAs, as well as other RNA substrates. The enzyme is also active with various mRNAs. M5C modifications in cytoplasmic and mitochondrial tRNAs. Three-dimensional L-shape structure of a tRNA with the positions of m5C modifications and the cognate methyltransferases responsible for installing them marked. The m5C modifications in cytoplasmic and mitochondrial tRNAs. Schematic secondary structure and three-dimensional L-shape structure of a tRNA with the positions of m5C modifications. The broad-spectrum methyltransferase NSUN2 has been suggested to recognize different features in its diverse substrate RNAs. The reported NSUN2-mediated m5C modifications in mRNAs typically lie within highly GC-rich regions, suggesting that the enzyme may preferentially bind such sequences. But all the known NSUN2-mediated m5C modifications in vtRNAs lie within a UCG motif, and mutagenic analysis of the NSUN2 target pre-tRNALeu reveals a consensus sequence of C/A/U32-U/A33-m5C34-A35-A36-G37 Homo sapiens ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview Pyrococcus horikoshii ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview. Three-dimensional L-shape structure of a tRNA with the positions of m5C modifications and the cognate methyltransferases responsible for installing them marked. The m5C modifications in cytoplasmic and mitochondrial tRNAs. Schematic secondary structure and three-dimensional L-shape structure of a tRNA with the positions of m5C modifications, determination of the interaction sites of NSUN6 with the discriminator base and additional base pairs in the acceptor stem and the D-loop, as observed by X-ray crystallography, overview. m5C72 modifications installed by NSUN6 lie within the acceptor stem of tRNA. NSUN6 forms extensive contacts with its substrate tRNAs. Binding of NSUN6 disrupts base pairing within the tRNA acceptor stem and promotes base-flipping of C71 to make the C5 atom of the C72 nucleotide, which is normally base paired with G1, accessible for methylation. NSUN6 also has a PUA domain that binds to the D-stem region of substrate tRNAs, as well as the non-genomically encoded CCA 3' end. Consistent with this binding mode, the presence of the CCA is found to be an essential pre-requisite for methylation of tRNACys and tRNAThr by NUSN6 Homo sapiens ? - - 89 2.1.1.202 additional information the CCA end is precisely recognized by hNSun6 primarily through the PUA domain, overview. The main chains of the residues (Arg126, Pro206 and Asp209) recognizes C74. Recognition for C75 comes from the main chain residues (Lys192 and Gly193) and the side chain residues (Lys192 and Asp209). The base moiety of C75 is stacked with the Tyr131 residue. Recognition for A76 is achieved mostly by the main chain of His129 and the side chain of Lys 192. Hydrophobic interactions with ambient aa residues, including Cys120, facilitate localization of the A76 base moiety. The discriminator base U73 binds to the RRM motif. C72 is recognized by the catalytic core. Multiple roles of the PUA domain in tRNA binding Homo sapiens ? - - 89 2.1.1.202 additional information Trm4b is active on the wild-type tRNA, its activity is slightly enhanced on C34A and completely abrogated on C49A, indicating that Trm4b has in vitro activity on tRNAProCGG and is specific for C49, which is in agreement with in vivo methylation data. Substrate specificity, comparison to trm4a, overview Schizosaccharomyces pombe ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview Pyrococcus horikoshii DSM 12428 ? - - 89 2.1.1.202 additional information Trm4b is active on the wild-type tRNA, its activity is slightly enhanced on C34A and completely abrogated on C49A, indicating that Trm4b has in vitro activity on tRNAProCGG and is specific for C49, which is in agreement with in vivo methylation data. Substrate specificity, comparison to trm4a, overview Schizosaccharomyces pombe ATCC 24843 ? - - 89 2.1.1.202 additional information C34 methylation depends on Trm4a in both tRNALeuCAA and tRNAProCGG. Substrate specificity, comparison to trm4b, overview Schizosaccharomyces pombe ATCC 24843 ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview Pyrococcus horikoshii NBRC 100139 ? - - 89 2.1.1.202 additional information Trm4b is active on the wild-type tRNA, its activity is slightly enhanced on C34A and completely abrogated on C49A, indicating that Trm4b has in vitro activity on tRNAProCGG and is specific for C49, which is in agreement with in vivo methylation data. Substrate specificity, comparison to trm4a, overview Schizosaccharomyces pombe 972 ? - - 89 2.1.1.202 additional information C34 methylation depends on Trm4a in both tRNALeuCAA and tRNAProCGG. Substrate specificity, comparison to trm4b, overview Schizosaccharomyces pombe 972 ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview Pyrococcus horikoshii JCM 9974 ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview Pyrococcus horikoshii ATCC 700860 ? - - 89 2.1.1.202 additional information susbtrate specificity of NSUN6, overview Pyrococcus horikoshii OT-3 ? - - 89 2.1.1.202 S-adenosyl-L-methionine + CpG-rich ssRNA (5'-CGCGCGCGCGCG-3') m5C methylation directly alters the phosphate backbone in CpG RNA duplex, leading to a C3'-endo to C2'-endo sugar pucker switch of the terminal residues under physiologically-relevant conditions. m5C triggers a B-to-Z DNA, but not A-to-Z, transformation in CpG DNA duplex. The m5C-probe has the sensitivity to detect a single m5C mark change Homo sapiens ? - ? 456441 2.1.1.202 S-adenosyl-L-methionine + cytosine in tRNA precursor - Arabidopsis thaliana S-adenosyl-L-homocysteine + 5-methylcytosine in tRNA precursor - ? 454524 2.1.1.202 S-adenosyl-L-methionine + cytosine in tRNALeu(CAA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine in tRNALeu(CAA) - ? 456453 2.1.1.202 S-adenosyl-L-methionine + cytosine15 in vtRNA1.3 - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine15 in vtRNA1.3 - ? 454525 2.1.1.202 S-adenosyl-L-methionine + cytosine27 in vtRNA1.2 - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine27 in vtRNA1.2 - ? 454526 2.1.1.202 S-adenosyl-L-methionine + cytosine27 in vtRNA1.3 - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine27 in vtRNA1.3 - ? 454527 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA cf. EC 2.1.1.203 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA cf. EC 2.1.1.203 Saccharomyces cerevisiae BY4741 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA - ? 411001 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA precursor mini-tRNALeu (composed of the anticodon stem-loop extended by the intron) is used to test the formation of m5C34. The formation of m5C34 is strictly intron dependent Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor - ? 412836 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA precursor the entire intron, or the total precursor tRNA structure, is required for efficient suppressor function of the resultant mature tRNA. The reduced suppressor phenotype is correlated with lack of a 5-methylcytosine modification of the anticodon wobble base Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor - ? 412836 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNA precursor yeast pre-tRNALeu Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor - ? 412836 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNALeu precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNALeu precursor - ? 434752 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNAPro(CGG) - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro(CGG) - ? 454533 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNAPro(CGG) - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro(CGG) - ? 454533 2.1.1.202 S-adenosyl-L-methionine + cytosine34 in tRNAPro(CGG) - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNAPro(CGG) - ? 454533 2.1.1.202 S-adenosyl-L-methionine + cytosine4( in tRNAPro(AGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAPro(AGG) - ? 454536 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA - ? 411004 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA - ? 411004 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA - ? 411004 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA - Saccharomyces cerevisiae BY4741 S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA - ? 411004 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA precursor - Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor - ? 412837 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA precursor mini-tRNAPhe (composed of the anticodon stem-loop extended by the intron) is used to test the formation of m5C40. The formation of m5C40 is strictly intron dependent Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor - ? 412837 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA precursor the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor - ? 412838 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNA precursor the enzymatic formation of m5C at position 40 in the anticodon stem is strictly dependent on the presence of the intron. Enzymatic formation of m5C40 is independent of the whole architecture of the tRNA molecule and takes place on a minisubstrate composed of the anticodon stem nd loop of yeast tRNAPhe prolonged by its natural 19 nt intron Saccharomyces cerevisiae Pp1001 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA precursor - ? 412838 2.1.1.202 S-adenosyl-L-methionine + cytosine40 in tRNAGly precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNAGly precursor - ? 434761 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA - Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA tRNATyr(GUA), tRNASer(AGA), and tRNAIle(UAU) are used to test the formation of m5C48 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA yeast tRNASer(AGE) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA - Saccharomyces cerevisiae BY4741 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNA - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 411005 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAAla(AGC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAla(AGC) - ? 454537 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAAla(CGC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAla(CGC) - ? 454538 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAAla(UGC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAla(UGC) - ? 454539 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAAsp precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAsp precursor - ? 434762 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAAsp(GUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAsp(GUC) - ? 454540 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAAspGTC - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAAspGTC - ? 434763 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGln(CUG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGln(CUG) - ? 454541 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGln(UUG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGln(UUG) - ? 454542 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGlu(CUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlu(CUC) - ? 454543 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGlu(UUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlu(UUC) - ? 454544 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGly precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly precursor - ? 434764 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGly(CCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly(CCC) - ? 454545 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGly(GCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly(GCC) - ? 454546 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGly(UCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGly(UCC) - ? 454547 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAGlyGCC - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAGlyGCC - ? 434765 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAHis - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis - ? 434766 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAHis purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis - ? 434766 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAHis - Saccharomyces cerevisiae BY4741 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis - ? 434766 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAHis(GUG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAHis(GUG) - ? 454548 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAIle(AAU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAIle(AAU) - ? 454549 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(AAG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(AAG) - ? 454550 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAA) - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(CAA) - ? 454552 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAA) - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(CAA) - ? 454552 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAA) - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(CAA) - ? 454552 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAA) precursor NSUN2-mediated methylation of C34 of tRNALeu(CAA) has been shown to occur exclusively on intron-containing tRNA precursors Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(CAA) precursor - ? 454551 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(CAG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(CAG) - ? 454553 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(UAA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(UAA) - ? 454554 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeu(UAG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeu(UAG) - ? 454555 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALeuCAA - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALeuCAA - ? 434767 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALys(CUU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALys(CUU) - ? 454556 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNALys(UUU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNALys(UUU) - ? 454557 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAMet(CAU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAMet(CAU) - ? 454558 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAPhe(GAA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAPhe(GAA) - ? 454559 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAPro(CGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAPro(CGG) - ? 454560 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAPro(UGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAPro(UGG) - ? 454561 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNASer(AGA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNASer(AGA) - ? 454562 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNASer(CGA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNASer(CGA) - ? 454563 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNASer(GCU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNASer(GCU) - ? 454564 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNASer(UGA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNASer(UGA) - ? 454565 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAThr(AGU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAThr(AGU) - ? 454566 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAThr(CGT) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAThr(CGT) - ? 454567 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAThr(UGU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAThr(UGU) - ? 454568 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNATyr(GUA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNATyr(GUA) - ? 454569 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAVal(AAC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAVal(AAC) - ? 454570 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAVal(CAC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAVal(CAC) - ? 454571 2.1.1.202 S-adenosyl-L-methionine + cytosine48 in tRNAVal(UAC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNAVal(UAC) - ? 454572 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA the enzyme is responsible for complete m5C methylation of yeast tRNA. The frequency of modification depends on the cytosine position in tRNA. At positions 34 and 40, m5C is found only in two yeast tRNAs (tRNALeu (CUA) and tRNAPhe (GAA), respectively), whereas most other elongator yeast tRNAs bear either m5C48 or m5C49, but never both in the same tRNA molecule Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA tRNAPhe(GAA) and tRNAAsp(GUC) are used to test the formation of m5C49 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA yeast tRNAAsp(GUC) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA - Saccharomyces cerevisiae BY4741 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA - ? 411006 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA yeast tRNA(GAA) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 412839 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNA yeast tRNA(GAA) Saccharomyces cerevisiae Pp1001 S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA - ? 412839 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAasp precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAsp precursor - ? 434768 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAAsp(GUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAsp(GUC) - ? 454573 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAAspGTC - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAAspGTC - ? 434769 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGln(CUG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGln(CUG) - ? 454574 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGln(UUG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGln(UUG) - ? 454575 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGlu(CUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlu(CUC) - ? 454576 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGlu(UUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlu(UUC) - ? 454577 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGly precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly precursor - ? 434770 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGly(CCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly(CCC) - ? 454578 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGly(GCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly(GCC) - ? 454579 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGly(UCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGly(UCC) - ? 454580 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAGlyGCC - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAGlyGCC - ? 434771 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNALeuCAA - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALeuCAA - ? 434772 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNALys(UUU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNALys(UUU) - ? 454581 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPhe(GAA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPhe(GAA) - ? 454582 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPro(AGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPro(AGG) - ? 454583 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPro(CGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPro(CGG) - ? 454584 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPro(CGG) - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPro(CGG) - ? 454584 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPro(CGG) - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPro(CGG) - ? 454584 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPro(CGG) - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPro(CGG) - ? 454584 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAPro(UGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAPro(UGG) - ? 454585 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAThr(AGU) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAThr(AGU) - ? 454586 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAVal(AAC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAVal(AAC) - ? 454587 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAVal(CAC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAVal(CAC) - ? 454588 2.1.1.202 S-adenosyl-L-methionine + cytosine49 in tRNAVal(UAC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNAVal(UAC) - ? 454589 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNA - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNA - ? 454598 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNA - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNA - ? 454598 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNA - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNA - ? 454598 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAAspGTC - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAAspGTC - ? 434773 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGlu(CUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlu(CUC) - ? 454590 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGlu(UUC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlu(UUC) - ? 454591 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGly precursor - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly precursor - ? 434774 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGly(CCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly(CCC) - ? 454592 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGly(GCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly(GCC) - ? 454593 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGly(UCC) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGly(UCC) - ? 454594 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAGlyGCC - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAGlyGCC - ? 434775 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAHis - Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis - ? 434776 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAHis purified tRNAHis, secondary structure of mature Saccharomyces cerevisiae tRNAHis, overview Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAHis - ? 434776 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNALeuCAA - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNALeuCAA - ? 434777 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAPro(AGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAPro(AGG) - ? 454595 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAPro(CGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAPro(CGG) - ? 454596 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAPro(CGG) - Schizosaccharomyces pombe S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAPro(CGG) - ? 454596 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAPro(CGG) - Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAPro(CGG) - ? 454596 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAPro(CGG) - Schizosaccharomyces pombe 972 S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAPro(CGG) - ? 454596 2.1.1.202 S-adenosyl-L-methionine + cytosine50 in tRNAPro(UGG) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine50 in tRNAPro(UGG) - ? 454597 2.1.1.202 S-adenosyl-L-methionine + cytosine59 in vtRNA1.2 - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine59 in vtRNA1.2 - ? 454599 2.1.1.202 S-adenosyl-L-methionine + cytosine59 in vtRNA1.3 - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine59 in vtRNA1.3 - ? 454600 2.1.1.202 S-adenosyl-L-methionine + cytosine69 in vtRNA1.1 - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine69 in vtRNA1.1 - ? 454601 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Pyrococcus horikoshii S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Pyrococcus horikoshii DSM 12428 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Pyrococcus horikoshii NBRC 100139 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Pyrococcus horikoshii JCM 9974 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Pyrococcus horikoshii ATCC 700860 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys - Pyrococcus horikoshii OT-3 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys - ? 454603 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys(GCA) - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys(GCA) - ? 454602 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNACys(GCA)-G2A:C71U - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNACys(GCA)-G2A:C71U - ? 456460 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Pyrococcus horikoshii S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Pyrococcus horikoshii DSM 12428 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Pyrococcus horikoshii NBRC 100139 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Pyrococcus horikoshii JCM 9974 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Pyrococcus horikoshii ATCC 700860 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604 2.1.1.202 S-adenosyl-L-methionine + cytosine72 in tRNAThr - Pyrococcus horikoshii OT-3 S-adenosyl-L-homocysteine + 5-methylcytosine72 in tRNAThr - ? 454604