2.1.1.178 additional information substrate recognition mechanism, overview Thermus thermophilus ? - ? 89 2.1.1.178 additional information substrate recognition mechanism, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ? - ? 89 2.1.1.178 S-adenosyl-L-methionine + cytosine1400 in 16S rRNA - Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA - ? 419569 2.1.1.178 S-adenosyl-L-methionine + cytosine1400 in 16S rRNA RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA - ? 419569 2.1.1.178 S-adenosyl-L-methionine + cytosine1400 in 16S rRNA - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA - ? 419569 2.1.1.178 S-adenosyl-L-methionine + cytosine1400 in 16S rRNA RsmF completely methylates C1400 when either 16S rRNA or 30S subunits are used as a substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1400 in 16S rRNA - ? 419569 2.1.1.178 S-adenosyl-L-methionine + cytosine1404 in 16S rRNA - Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA - ? 419570 2.1.1.178 S-adenosyl-L-methionine + cytosine1404 in 16S rRNA RsmF methylates C1404 to about 35% with 16S rRNA and completely with 30S subunits Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1404 in 16S rRNA - ? 419570 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA - Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA - Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 is located at a functionally active region of the 30 S subunit interface close to the P site. YebU-directed methylation of this nucleotide seems to be conserved in bacteria Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA purified recombinant YebU protein retains its specificity for C1407 in vitro, and methylates 30 S subunits (but not naked 16 S rRNA or 70 S ribosomes) isolated from yebU knockout strains Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA Escherichia coli S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits Thermus thermophilus S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA - Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA nucleotide C1407 forms part of the aminoglycoside binding pocket of the 16S rRNA Escherichia coli BW25113 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA - Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA RsmF produces only trace amounts of methylation of C1407 with 16S rRNA and about 75% with 30S subunits Thermus thermophilus HB8 / ATCC 27634 / DSM 579 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341 2.1.1.178 S-adenosyl-L-methionine + cytosine1407 in 16S rRNA - Escherichia coli MM294 S-adenosyl-L-homocysteine + 5-methylcytosine1407 in 16S rRNA - ? 407341