2.1.1.157	2 S-adenosyl-L-methionine + sarcosine	(overall reaction)	Halorhodospira halochloris	2 S-adenosyl-L-homocysteine + betaine	-	?	424074	
2.1.1.157	additional information	the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process	Halorhodospira halochloris	?	-	?	89	
2.1.1.157	additional information	the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process	Actinopolyspora halophila	?	-	?	89	
2.1.1.157	additional information	the enzyme catalyzes the last 2 steps of betaine synthesis from glycine in a 3-step process, betaine is required for balancing osmotic pressure under high salt living conditions	Halorhodospira halochloris	?	-	?	89	
2.1.1.157	additional information	the enzyme is involved in the alternate biosynthesis of betaine, which is an important osmoprotectant and is synthesized in response to abiotic stress	Aphanothece halophytica	?	-	?	89	
2.1.1.157	additional information	enzyme shows strict substrate specificity	Aphanothece halophytica	?	-	?	89	
2.1.1.157	additional information	the enzyme has overlapping activities with the enzyme catalyzing the prior reaction steps	Halorhodospira halochloris	?	-	?	89	
2.1.1.157	additional information	the enzyme has overlapping activities with the enzyme catalyzing the prior reaction steps	Actinopolyspora halophila	?	-	?	89	
2.1.1.157	additional information	the enzyme shows strict specificity for glycine and sarcosine as substrates, no activity with ethanolamine, monomethylethanolamine, nor several L-amino acid, overview	Halorhodospira halochloris	?	-	?	89	
2.1.1.157	additional information	the broad substrate methyltransferase, glycine sarcosine dimethylglycine methyltransferase, GSDMT, purified from the halophilic methanoarchaeon Methanohalophilus portucalensis strain FDF1, has been shown to possess glycine N-methyltransferase (GMT), sarcosine N-methyltransferase (SMT) and dimethylglycine N-methyltransferase (DMT) activities. SDMT possesses sarcosine N-methyltransferase (SMT) and dimethylglycine N-methyltransferase (DMT) activities	Methanohalophilus portucalensis	?	-	?	89	
2.1.1.157	additional information	the enzyme from Methanohalophilus portucalensis possesses sarcosine N-methyltransferase (SMT) and dimethylglycine N-methyltransferase (DMT) activities	Methanohalophilus portucalensis	?	-	?	89	
2.1.1.157	additional information	the broad substrate methyltransferase, glycine sarcosine dimethylglycine methyltransferase, GSDMT, purified from the halophilic methanoarchaeon Methanohalophilus portucalensis strain FDF1, has been shown to possess glycine N-methyltransferase (GMT), sarcosine N-methyltransferase (SMT) and dimethylglycine N-methyltransferase (DMT) activities. SDMT possesses sarcosine N-methyltransferase (SMT) and dimethylglycine N-methyltransferase (DMT) activities	Methanohalophilus portucalensis FDF1	?	-	?	89	
2.1.1.157	additional information	the enzyme from Methanohalophilus portucalensis possesses sarcosine N-methyltransferase (SMT) and dimethylglycine N-methyltransferase (DMT) activities	Methanohalophilus portucalensis FDF1	?	-	?	89	
2.1.1.157	S-adenosyl-L-methionine + glycine	low activity	Aphanothece halophytica	S-adenosyl-L-homocysteine + sarcosine	-	?	319640	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Myxococcus xanthus	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Galdieria sulphuraria	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Aphanothece halophytica	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Halorhodospira halochloris	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Actinopolyspora halophila	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Methanohalophilus portucalensis	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Halorhodospira halochloris	S-adenosyl-L-homocysteine + betaine	i.e. N,N,N-trimethylglycine	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Aphanothece halophytica	S-adenosyl-L-homocysteine + betaine	i.e. N,N,N-trimethylglycine	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	preferred substrate	Actinopolyspora halophila	S-adenosyl-L-homocysteine + betaine	i.e. N,N,N-trimethylglycine	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	(1b)	Halorhodospira halochloris	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Methanohalophilus portucalensis FDF1	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Aphanothece halophytica 7418	S-adenosyl-L-homocysteine + betaine	-	?	375293	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine	Methanohalophilus portucalensis	S-adenosyl-L-homocysteine + N,N,N-trimethylglycine	-	?	386683	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine	Methanohalophilus portucalensis FDF1	S-adenosyl-L-homocysteine + N,N,N-trimethylglycine	-	?	386683	
2.1.1.157	S-adenosyl-L-methionine + N,N-dimethylglycine	-	Methanohalophilus portucalensis	S-adenosyl-L-homocysteine + betaine + H+	-	?	406619	
2.1.1.157	S-adenosyl-L-methionine + N-methylglycine	synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine	Methanohalophilus portucalensis	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	386685	
2.1.1.157	S-adenosyl-L-methionine + N-methylglycine	synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine	Methanohalophilus portucalensis FDF1	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	386685	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Myxococcus xanthus	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Galdieria sulphuraria	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Aphanothece halophytica	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Halorhodospira halochloris	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Actinopolyspora halophila	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Methanohalophilus portucalensis	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	i.e. methylglycine	Halorhodospira halochloris	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	i.e. methylglycine	Aphanothece halophytica	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	about 30% of the activity with N,N-dimethylglycine	Methanohalophilus portucalensis	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	(1a)	Halorhodospira halochloris	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Methanohalophilus portucalensis FDF1	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296	
2.1.1.157	S-adenosyl-L-methionine + sarcosine	-	Aphanothece halophytica 7418	S-adenosyl-L-homocysteine + N,N-dimethylglycine	-	?	375296