1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ - Methanothermococcus thermolithotrophicus 2 H2 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB - ? 461455 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ - Methanothermococcus thermolithotrophicus DSM 2095 2 H2 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB - ? 461455 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanothermobacter wolfeii 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanomassiliicoccus luminyensis 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanothermococcus thermolithotrophicus 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanocaldococcus jannaschii 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanospirillum hungatei 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanocaldococcus jannaschii NBRC 100440 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanocaldococcus jannaschii DSM 2661 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanocaldococcus jannaschii ATCC 43067 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanocaldococcus jannaschii JAL-1 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanothermococcus thermolithotrophicus DSM 2095 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 2 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + 2 H+ - Methanocaldococcus jannaschii JCM 10045 2 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 461456 1.8.7.3 additional information Hdr receives two electrons from the oxidation of H2 through MvhAGD [NiFe]-hydrogenase, cf. EC 1.8.98.4 Methanothermococcus thermolithotrophicus ? - - 89 1.8.7.3 additional information heterodisulfide reductase (HdrABC) reduces the disulfide bond with electrons supplied from the oxidation of 2H2 or 2HCO2H catalyzed by F420-independent hydrogenase or Fdh. The exergonic reduction of CoMS-SCoB drives the endergonic reduction of CO2 in the first step via FBEB by HdrABC Methanothermococcus thermolithotrophicus ? - - 89 1.8.7.3 additional information thiols are only produced when membranes of Methanomassiliicoccus luminyensis, HdrD, Fd, and IOR are present. In the absence of Fd, HdrD, or washed membranes, thiol formation is very low indicating that all above-mentioned components are necessary for an effective electron transfer from Fdred to CoM-S-S-CoB. Fd:heterodisulfide reductase activity is measured by the oxidation of Fdred as the initial reaction and thiol formation by CoM-S-S-CoB reduction as a final reaction of the proposed electron transport chain. At least one membrane-bound enzyme is needed for electron transport Methanomassiliicoccus luminyensis ? - - 89 1.8.7.3 additional information Hdr receives two electrons from the oxidation of H2 through MvhAGD [NiFe]-hydrogenase, cf. EC 1.8.98.4 Methanothermococcus thermolithotrophicus DSM 2095 ? - - 89 1.8.7.3 additional information heterodisulfide reductase (HdrABC) reduces the disulfide bond with electrons supplied from the oxidation of 2H2 or 2HCO2H catalyzed by F420-independent hydrogenase or Fdh. The exergonic reduction of CoMS-SCoB drives the endergonic reduction of CO2 in the first step via FBEB by HdrABC Methanothermococcus thermolithotrophicus DSM 2095 ? - - 89 1.8.7.3 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - Methanosarcina acetivorans reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - ? 442504 1.8.7.3 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - Methanosarcina acetivorans reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - r 442504 1.8.7.3 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - Methanothermobacter marburgensis reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - ? 442504 1.8.7.3 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - Acidithiobacillus caldus reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - ? 442504 1.8.7.3 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - Methanomassiliicoccus luminyensis reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - ? 442504 1.8.7.3 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - Methanomassiliicoccus luminyensis DSM 25720 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - ? 442504 1.8.7.3 oxidized methyl viologen + CoB + CoM - Methanomassiliicoccus luminyensis reduced methyl viologen + CoM-S-S-CoB + H+ - ? 443823 1.8.7.3 oxidized methyl viologen + CoB + CoM - Methanomassiliicoccus luminyensis DSM 25720 reduced methyl viologen + CoM-S-S-CoB + H+ - ? 443823 1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - Desulfovibrio vulgaris oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 442533 1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - Methanosarcina acetivorans oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - r 442533 1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB + H+ - Desulfovibrio vulgaris ATCC 29579 oxidized ferredoxin [iron-sulfur] cluster + CoB + CoM - ? 442533 1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+ - Desulfovibrio vulgaris oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC - ? 442534 1.8.7.3 reduced ferredoxin [iron-sulfur] cluster + DsrC-S-S-DsrC + H+ - Desulfovibrio vulgaris ATCC 29579 oxidized ferredoxin [iron-sulfur] cluster + DsrC + DsrC - ? 442534