1.7.1.17 1,2-naphthoquinone + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis ? - ? 461796 1.7.1.17 1,2-naphthoquinone + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis A01 ? - ? 461796 1.7.1.17 1,4-naphthoquinone + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis ? - ? 461805 1.7.1.17 1,4-naphthoquinone + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis A01 ? - ? 461805 1.7.1.17 1-(2-pyridylazo)-2-naphthol + 2 NADH + 2 H+ - Bacillus sp. B29 pyridin-2-amine + 1-aminonaphthalen-2-ol + 2 NAD+ - ? 442693 1.7.1.17 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ i.e. azo dye methyl red Escherichia coli anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ - ? 440085 1.7.1.17 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ i.e. azo dye methyl red, via a diazene intermediate Escherichia coli anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ - ? 440085 1.7.1.17 2-(4-dimethylaminophenylazo) benzoic acid + NADH + H+ i.e. methyl red Rhodococcus opacus N,N'-dimethyl-p-phenylenediamine + 2-aminobenzoic acid + NAD+ - ? 440618 1.7.1.17 2-(4-dimethylaminophenylazo) benzoic acid + NADH + H+ i.e. methyl red Rhodococcus opacus 1CP N,N'-dimethyl-p-phenylenediamine + 2-aminobenzoic acid + NAD+ - ? 440618 1.7.1.17 4'-(diethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. ethyl red Geobacillus stearothermophilus 2-aminobenzoic acid + N,N-diethyl-p-phenylenediamine + 2 NAD+ - ? 442877 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ - ? 442878 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Escherichia coli 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ - ? 442878 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Geobacillus stearothermophilus 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ - ? 442878 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Bacillus sp. B29 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ - ? 442878 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 700802 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ - ? 442878 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 27274 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+ - ? 442878 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+ - ? 442879 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 19433 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+ - ? 442879 1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 27274 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+ - ? 442879 1.7.1.17 4-(dimethylamino)azobenzene + NADH + H+ - Rhodococcus opacus N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ - ? 440113 1.7.1.17 4-(dimethylamino)azobenzene + NADH + H+ i.e. methyl yellow Rhodococcus opacus N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ - ? 440113 1.7.1.17 4-(dimethylamino)azobenzene + NADH + H+ - Rhodococcus opacus 1CP N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ - ? 440113 1.7.1.17 4-(dimethylamino)azobenzene + NADH + H+ i.e. methyl yellow Rhodococcus opacus 1CP N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ - ? 440113 1.7.1.17 7-nitrocoumarin-3-carboxylic acid + 2 NADH + 2 H+ via 7-nitrosocoumarin-3-carboxylic acid and 7-hydroxylaminocoumarin-3-carboxylic acid intermediates Escherichia coli 7-aminocoumarin-3-carboxylic acid + NAD+ + H2O - ? 440978 1.7.1.17 7-nitrocoumarin-3-carboxylic acid + NADH + H+ - Escherichia coli 7-aminocoumarin-3-carboxylic acid + NAD+ + H2O - ? 440127 1.7.1.17 acid red 88 + 2 NADH + 2 H+ - Geobacillus stearothermophilus 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NAD+ - ? 443013 1.7.1.17 acid red 88 + 2 NADH + 2 H+ - Bacillus sp. B29 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NAD+ - ? 443013 1.7.1.17 acid red 88 + NADH + H+ - Bacillus smithii ? + NAD+ - r 425889 1.7.1.17 acid red 88 + NADH + H+ - Bacillus smithii DSM 4216 ? + NAD+ - r 425889 1.7.1.17 Allura Red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ? - ? 462223 1.7.1.17 Allura Red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus 1CP ? - ? 462223 1.7.1.17 amaranth + 2 NADH + 2 H+ - Escherichia coli 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Enterococcus faecalis 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Bacillus cereus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Enterococcus avium 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Chromobacterium violaceum 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Enterococcus faecalis ATCC 19433 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADH + 2 H+ - Chromobacterium violaceum ATCC 12472 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+ - ? 443038 1.7.1.17 amaranth + 2 NADPH + 2 H+ - Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NADP+ - ? 443039 1.7.1.17 anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ - Staphylococcus sp. MEH038S 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH + 2 H+ - ? 461528 1.7.1.17 brilliant black + 2 NADH + 2 H+ - Escherichia coli ? - ? 462314 1.7.1.17 brilliant black + 2 NADH + 2 H+ - Enterococcus faecalis ? - ? 462314 1.7.1.17 brilliant black + 2 NADH + 2 H+ - Bacillus cereus ? - ? 462314 1.7.1.17 Brilliant Black + NADH + H+ low activity Rhodococcus opacus ? - ? 462315 1.7.1.17 Brilliant Black + NADH + H+ low activity Rhodococcus opacus 1CP ? - ? 462315 1.7.1.17 congo red + 2 NADH + 2 H+ - Geobacillus stearothermophilus ? + 2 NAD+ - ? 443396 1.7.1.17 Congo red + NADH + H+ - Bacillus smithii ? + NAD+ - r 426135 1.7.1.17 Congo red + NADH + H+ - Bacillus smithii DSM 4216 ? + NAD+ - r 426135 1.7.1.17 ethyl red + 2 NADH + 2 H+ - Escherichia coli anthranilate + N,N-diethyl-1,4-phenylenediamine + 2 NAD+ - ? 441376 1.7.1.17 fast red E + 2 NADH + 2 H+ - Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NAD+ - ? 443555 1.7.1.17 fast red E + 2 NADPH + 2 H+ - Lysinibacillus sphaericus 4-aminonaphthalene-1-sulfonic acid + 5-amino-6-hydroxynaphthalene-2-sulfonic acid + 2 NADP+ - ? 443556 1.7.1.17 menadione + 2 NADH + 2 H+ - Escherichia coli ? + 2 NAD+ - ? 441603 1.7.1.17 menadione + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis ? - ? 462692 1.7.1.17 menadione + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis A01 ? - ? 462692 1.7.1.17 methyl orange + 2 NADH + 2 H+ - Chromobacterium violaceum ? - ? 462706 1.7.1.17 methyl orange + 2 NADH + 2 H+ - Chromobacterium violaceum ATCC 12472 ? - ? 462706 1.7.1.17 methyl orange + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ? - ? 405836 1.7.1.17 methyl orange + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus 1CP ? - ? 405836 1.7.1.17 methyl orange + NADH + H+ - Bacillus smithii ? + NAD+ - r 441623 1.7.1.17 methyl orange + NADH + H+ - Bacillus smithii DSM 4216 ? + NAD+ - r 441623 1.7.1.17 methyl red + 2 NADH + 2 H+ - Chromobacterium violaceum ? - ? 462714 1.7.1.17 methyl red + 2 NADH + 2 H+ - Chromobacterium violaceum ATCC 12472 ? - ? 462714 1.7.1.17 methyl red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ? - ? 405839 1.7.1.17 methyl red + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus 1CP ? - ? 405839 1.7.1.17 methyl red + NADH + H+ - Bacillus smithii ? + NAD+ - r 405840 1.7.1.17 methyl red + NADH + H+ - Bacillus smithii DSM 4216 ? + NAD+ - r 405840 1.7.1.17 Methyl Red + NADH + H+ - Shewanella oneidensis 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ - ? 462715 1.7.1.17 additional information active site structure analysis and substrate specificity, Azo compound binding structure, overview Escherichia coli ? - ? 89 1.7.1.17 additional information AzoR utilizes NADH but not NADPH as an electron donor and binds FMN as a flavin cofactor Escherichia coli ? - ? 89 1.7.1.17 additional information no activity with Ponceau SX. Recombinant AcpD reaction follows a ping-pong mechanism requiring 2 mol of NADH to reduce 1 mol of methyl red into 2-aminobenzoic acid and N,N'-dimethyl-p-phenylenediamine. On the other hand, the gene product cannot convert holo-acyl carrier protein into the apo form under either in vitro or in vivo conditions. These data indicate that the acpD gene product is not acyl carrier protein phosphodiesterase but an azoreductase Escherichia coli ? - ? 89 1.7.1.17 additional information enzyme additionally acts as nitroreductase and selectively reduces the nitro group of 2-nitrophenol, 4-nitrobenzoic acid, 2-nitro-benzaldehyde and 3-nitrophenol Lysinibacillus sphaericus ? - ? 89 1.7.1.17 additional information free enzyme has no activity with azobenzene, 4-phenylazophenol, Allura Red, Amido Black, Reactive Orange, Para Red, Chlorazol Black and Methyl Orange Rhodococcus opacus ? - - 89 1.7.1.17 additional information the enzyme shows a negligible ability to use molecular oxygen as an electron acceptor Alkalihalobacillus wakoensis ? - - 89 1.7.1.17 additional information the enzyme shows a negligible ability to use molecular oxygen as an electron acceptor Alkalihalobacillus wakoensis A01 ? - - 89 1.7.1.17 additional information free enzyme has no activity with azobenzene, 4-phenylazophenol, Allura Red, Amido Black, Reactive Orange, Para Red, Chlorazol Black and Methyl Orange Rhodococcus opacus 1CP ? - - 89 1.7.1.17 orange G + 2 NADH + 2 H+ - Lysinibacillus sphaericus aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NAD+ - ? 443811 1.7.1.17 orange G + 2 NADPH + 2 H+ - Lysinibacillus sphaericus aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+ - ? 443812 1.7.1.17 orange G + 2 NADPH + 2 H+ - Enterococcus faecalis aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+ - ? 443812 1.7.1.17 orange G + 2 NADPH + 2 H+ - Enterococcus faecalis ATCC 19433 aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+ - ? 443812 1.7.1.17 orange I + 2 NADH + 2 H+ - Geobacillus stearothermophilus 4-aminobenzene-1-sulfonic acid + 4-aminonaphthalen-1-ol + 2 NAD+ - ? 443813 1.7.1.17 orange I + 2 NADH + 2 H+ - Bacillus sp. B29 4-aminobenzene-1-sulfonic acid + 4-aminonaphthalen-1-ol + 2 NAD+ - ? 443813 1.7.1.17 Orange I + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis ? - ? 462871 1.7.1.17 Orange I + 2 NADH + 2 H+ - Alkalihalobacillus wakoensis A01 ? - ? 462871 1.7.1.17 orange II + 2 NADPH + 2 H+ - Enterococcus faecalis 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NADP+ + 2 NAD+ - ? 443814 1.7.1.17 orange II + 2 NADPH + 2 H+ - Enterococcus faecalis ATCC 19433 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NADP+ + 2 NAD+ - ? 443814 1.7.1.17 orange II + NADH + H+ - Bacillus smithii ? + NAD+ - r 426881 1.7.1.17 orange II + NADH + H+ - Bacillus smithii DSM 4216 ? + NAD+ - r 426881 1.7.1.17 ponceau BS + 2 NADH + 2 H+ - Enterococcus faecalis ? + 2 NAD+ - ? 443853 1.7.1.17 ponceau BS + 2 NADH + 2 H+ - Enterococcus faecalis ATCC 19433 ? + 2 NAD+ - ? 443853 1.7.1.17 ponceau S + 2 NADH + 2 H+ - Enterococcus faecalis ? + 2 NAD+ - ? 443854 1.7.1.17 Reactive black 5 + 2 NADH + 2 H+ - Staphylococcus sp. MEH038S n-hydroxy-benzenamine + dimethyl phthalate + phenylethyl alcohol + 4-methylphenol + 3-(methylthio)propanoic acid methyl ester + 2 NAD+ - ? 462960 1.7.1.17 Reactive Orange + NADH + H+ substrate for immobilized enzyme (catalyst meso-cellular foam-A) only Rhodococcus opacus ? - ? 462975 1.7.1.17 sunset yellow + 2 NADH + 2 H+ - Enterococcus avium ? - ? 463042 1.7.1.17 tartrazine + 2 NADH + 2 H+ - Escherichia coli ? - ? 463049 1.7.1.17 tartrazine + 2 NADH + 2 H+ - Enterococcus faecalis ? - ? 463049 1.7.1.17 tartrazine + 2 NADH + 2 H+ - Bacillus cereus ? - ? 463049