1.4.4.2 glycine + H-protein-lipoyllysine - Mus musculus H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine - Escherichia coli H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine - Homo sapiens H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine - Arabidopsis thaliana H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine - Trichomonas vaginalis H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine - Synechocystis sp. PCC 6803 H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine - Synechocystis sp. H-protein-S-aminomethyldihydrolipoyllysine + CO2 - ? 386491 1.4.4.2 glycine + H-protein-lipoyllysine P-protein is the actual glycine-decarboxylating enzyme and uses pyridoxal 5'-phosphate as a cofactor. CO2 is released in the reaction and the residual aminomethyl group is bound to the oxidized lipoamide arm of H-protein Synechocystis sp. H-protein-S-aminomethyldihydrolipoyllysine + CO2 the methylene group is accepted by tetrahydrofolate to yield methylene tetrahydrofolate, a major cofactor in one-carbon metabolism ? 386491 1.4.4.2 glycine + His-tagged H-apoprotein very low activity Synechocystis sp. ? - ? 397347 1.4.4.2 glycine + lipoamide - Trichomonas vaginalis ? - ? 451633 1.4.4.2 glycine + lipoate very low activity Synechocystis sp. ? - ? 397348 1.4.4.2 glycine + lipoylated H-apoprotein very low activity Synechocystis sp. ? - ? 397349 1.4.4.2 glycine + lipoylated His-tagged H-apoprotein very low activity Synechocystis sp. ? - ? 397350 1.4.4.2 glycine + lipoylprotein - Saccharomyces cerevisiae S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein - Pisum sativum S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein - Zea mays S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein - Flaveria trinervia S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein - C3-plants S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein - Gallus gallus S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein reaction is stimulated by lipoic acid which is a functional group of the H-protein Gallus gallus S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein glycine decarboxylation catalyzed by P-protein alone is extremely low Gallus gallus S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Gallus gallus S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Rattus norvegicus S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Bos taurus S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Triticum aestivum S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Pisum sativum S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Arthrobacter globiformis S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Peptoclostridium acidaminophilum S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Parvimonas micra S-aminomethyldihydrolipoylprotein + CO2 - ? 259167 1.4.4.2 glycine + rTvH1 protein - Trichomonas vaginalis ? - ? 451634 1.4.4.2 glycine + rTvH2 protein - Trichomonas vaginalis ? - ? 451635 1.4.4.2 glycine + [glycine-cleavage complex H protein]-N6-lipoyl-L-lysine - Homo sapiens [glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine + CO2 - r 440258 1.4.4.2 glycine + [glycine-cleavage complex H protein]-N6-lipoyl-L-lysine reversible reaction Homo sapiens [glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine + CO2 - r 440258 1.4.4.2 H1 protein substrate for L protein Trichomonas vaginalis ? - ? 392179 1.4.4.2 H2 protein substrate for L protein Trichomonas vaginalis ? - ? 392180 1.4.4.2 additional information - Arthrobacter globiformis ? - ? 89 1.4.4.2 additional information P-protein also catalyzes exchange of carbonyl carbon of glycine with CO2, reaction greatly stimulated by addition of H protein Gallus gallus ? - ? 89 1.4.4.2 additional information P-protein also catalyzes exchange of carbonyl carbon of glycine with CO2, reaction greatly stimulated by addition of H protein Pisum sativum ? - ? 89 1.4.4.2 additional information the enzyme is a component of the reversible glycine cleavage system, previously known as glycine synthase Rattus norvegicus ? - ? 89 1.4.4.2 additional information glycine decarboxylase does not occur as a complex of all four proteins, but L-protein takes part in both glycine decarboxylase as well as in glycine reductase reaction Peptoclostridium acidaminophilum ? - ? 89 1.4.4.2 additional information the glycine cleavage system consists of 4 protein components: 1. P-protein is a pyridoxal containing protein: a Schiff base is formed between the hydroxyl group of the pyridoxal phosphate and the alpha-NH2 of glycine, the amino group and the alpha-carbon of the glycine are transferred to the lipoamide cofactor of the second enzyme of the complex the H-protein, the alpha-carbonyl group of glycine is lost as CO2, 2. H-protein, 3. T-protein: catalyzes the passage of alpha-carbon from lipoamide of H protein to tetrahydrofolate, alpha-NH2 from glycine is lost as NH4+, 4. L-protein: catalyzes oxidation of reduced lipoamide back to its original form with concomitant reduction of NAD+ to NADH Gallus gallus ? - ? 89 1.4.4.2 additional information nonketotic hyperglycinaemia is an autosomal recessive disorder of glycine metabolism caused by a deficiency in the mitochondrial glycine cleavage enzyme. The majority of cases are caused by mutations in the P-protein Homo sapiens ? - ? 89 1.4.4.2 additional information in transgenic potato plants with an antisense reduction in P-protein of GDC, the decrease in photorespiratory decarboxylation is compensated for by an increase in respiratory decarboxylation in the light Solanum tuberosum ? - ? 89 1.4.4.2 additional information the GDC H-protein (GLDH) has no catalytic activity itself but interacts via its lipoyl arm one after the other with the three other GDC subunits, P-protein (EC 1.4.4.2), T-protein (EC 2.1.2.10), and L-protein (EC 1.8.1.4) Flaveria trinervia ? - ? 89 1.4.4.2 additional information the GDC H-protein is a substrate for P-protein of glycine decarboxylase Flaveria trinervia ? - ? 89 1.4.4.2 additional information presence of excess pyridoxal 5'-phosphate is used in the assay mixture to facilitate the displacement of the quinonoid intermediate in the absence of an acceptor for the methylamine moiety (of decarboxylated glycine) to allow multiple enzyme turnovers. Mass spectrometry and 13C NMR spectroscopy are used to determine the products of glycine decarboxylation. The assay reaction mixtures are incubated for 1 week at pH 7.0, 25°C Homo sapiens ? - ? 89 1.4.4.2 P2 protein-S-aminomethyldihydrolipoyl-L-lysine + bicarbonate - Parvimonas micra ? - r 451636