1.4.3.19 2-iminoacetate + H2O - Marinomonas mediterranea glyoxylate + NH3 - ? 440096 1.4.3.19 2-iminoacetate + H2O - Bacillus subtilis glyoxylate + NH3 - ? 440096 1.4.3.19 2-iminoacetate + H2O - Bacillus cereus glyoxylate + NH3 - ? 440096 1.4.3.19 2-iminoacetate + H2O - Geobacillus kaustophilus glyoxylate + NH3 - ? 440096 1.4.3.19 2-iminoacetate + H2O - Bacillus subtilis 168 glyoxylate + NH3 - ? 440096 1.4.3.19 cephalosporin C + H2O + O2 - Geobacillus kaustophilus ? - ? 396685 1.4.3.19 cephalosporin C + H2O + O2 - Geobacillus kaustophilus HTA426 ? - ? 396685 1.4.3.19 cyclopropylglycine + H2O + O2 - Bacillus subtilis ? - ? 289411 1.4.3.19 cyclopropylglycine + H2O + O2 enzyme is required for the biosynthesis of the thiazole moiety of thiamine diphosphate Bacillus subtilis ? - ? 289411 1.4.3.19 D-2-aminobutyrate + H2O + O2 2.2% of the activity with sarcosine Bacillus subtilis ? + NH3 + H2O2 - ? 289402 1.4.3.19 D-2-aminobutyrate + H2O + O2 as active as sarcosine Bacillus subtilis 2-oxobutyrate + H2O2 - ? 372812 1.4.3.19 D-4-hydroxyphenylglycine + H2O + O2 0.6% activity compared to glycine Marinomonas mediterranea ? - ? 424738 1.4.3.19 D-4-hydroxyphenylglycine + H2O + O2 0.6% activity compared to glycine Marinomonas mediterranea MMB-1 ? - ? 424738 1.4.3.19 D-Ala + H2O + O2 7.4% of the activity with sarcosine Bacillus subtilis pyruvate + NH3 + H2O2 - ? 289401 1.4.3.19 D-Ala + H2O + O2 no activity with D-Ala Bacillus subtilis pyruvate + NH3 + H2O2 - ? 289401 1.4.3.19 D-Ala + H2O + O2 about 115% of the activity with sarcosine Bacillus subtilis pyruvate + NH3 + H2O2 - ? 289401 1.4.3.19 D-Ala + H2O + O2 - Geobacillus kaustophilus HTA426 ? + NH3 + H2O2 - ? 404765 1.4.3.19 D-Ala + H2O + O2 40% of the activity with sarcosine Bacillus subtilis ? + NH3 + H2O2 - ? 404765 1.4.3.19 D-alanine + H2O + O2 - Bacillus subtilis pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 - Geobacillus kaustophilus pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 - Bacillus cereus pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 0.2% activity compared to glycine Marinomonas mediterranea pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 - Bacillus cereus ATCC 14579 pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 0.2% activity compared to glycine Marinomonas mediterranea MMB-1 pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 - Bacillus subtilis R5 pyruvate + NH3 + H2O2 - ? 370910 1.4.3.19 D-alanine + H2O + O2 - Bacillus cereus ? - ? 396833 1.4.3.19 D-alanine + H2O + O2 10% activity compared to glycine Pseudomonas putida ? - ? 396833 1.4.3.19 D-alanine + H2O + O2 10% activity compared to glycine Pseudomonas putida KT 2240 ? - ? 396833 1.4.3.19 D-alanine + H2O + O2 - Bacillus cereus HYC-7 ? - ? 396833 1.4.3.19 D-aminobutanoate + H2O + O2 30% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 404771 1.4.3.19 D-Arg + H2O + O2 about 35% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 372828 1.4.3.19 D-arginine + H2O + O2 - Geobacillus kaustophilus ? - ? 396840 1.4.3.19 D-arginine + H2O + O2 - Geobacillus kaustophilus HTA426 ? - ? 396840 1.4.3.19 D-aspartate + H2O + O2 - Geobacillus kaustophilus ? - ? 396843 1.4.3.19 D-aspartate + H2O + O2 - Geobacillus kaustophilus HTA426 ? - ? 396843 1.4.3.19 D-glutamate + H2O + O2 0.2% activity compared to glycine Marinomonas mediterranea ? - ? 424756 1.4.3.19 D-His + H2O + O2 about 25% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 372920 1.4.3.19 D-Ile + H2O + O2 about 30% of the activity with sarcosine Bacillus subtilis 3-methyl-2-oxopentanoate + H2O2 - ? 372924 1.4.3.19 D-Leu + H2O + O2 about 35% of the activity with sarcosine Bacillus subtilis 4-methyl-2-oxopentanoate + H2O2 - ? 372935 1.4.3.19 D-lysine + H2O + O2 0.2% activity compared to glycine Marinomonas mediterranea ? - ? 424759 1.4.3.19 D-methionine + H2O + O2 - Geobacillus kaustophilus 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2 - ? 396916 1.4.3.19 D-methionine + H2O + O2 - Geobacillus kaustophilus HTA426 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2 - ? 396916 1.4.3.19 D-methionine + H2O + O2 25% of the activity with sarcosine Bacillus subtilis 4-(methylsulfanyl)-2-oxobutanoic acid + NH3 + H2O2 - ? 396916 1.4.3.19 D-methionine + H2O + O2 - Bacillus subtilis ? - ? 396919 1.4.3.19 D-norvaline + H2O + O2 - Geobacillus kaustophilus 2-oxopentanoic acid + NH3 + H2O2 - ? 396922 1.4.3.19 D-norvaline + H2O + O2 - Geobacillus kaustophilus HTA426 2-oxopentanoic acid + NH3 + H2O2 - ? 396922 1.4.3.19 D-phenylalanine + H2O + O2 - Geobacillus kaustophilus phenylpyruvate + NH3 + H2O2 - ? 394824 1.4.3.19 D-phenylalanine + H2O + O2 - Geobacillus kaustophilus HTA426 phenylpyruvate + NH3 + H2O2 - ? 394824 1.4.3.19 D-phenylalanine + H2O + O2 - Bacillus subtilis phenylpyruvate + NH3 + H2O2 - ? 394824 1.4.3.19 D-pipecolate + H2O + O2 about 85% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 372959 1.4.3.19 D-pipecolate + H2O + O2 70% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 372959 1.4.3.19 D-pipecolate + H2O + O2 - Geobacillus kaustophilus ? - ? 396926 1.4.3.19 D-pipecolate + H2O + O2 - Geobacillus kaustophilus HTA426 ? - ? 396926 1.4.3.19 D-pipecolate + H2O + O2 - Bacillus subtilis ? - ? 396926 1.4.3.19 D-Pro + H2O + O2 - Bacillus subtilis ? + NH3 + H2O2 - ? 289403 1.4.3.19 D-Pro + H2O + O2 - Geobacillus kaustophilus HTA426 ? + NH3 + H2O2 - ? 289403 1.4.3.19 D-Pro + H2O + O2 15.1% of the activity with sarcosine Bacillus subtilis ? + NH3 + H2O2 - ? 289403 1.4.3.19 D-Pro + H2O + O2 110% of the activity with sarcosine Bacillus subtilis ? + NH3 + H2O2 - ? 289403 1.4.3.19 D-Pro + H2O + O2 about 120% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 372963 1.4.3.19 D-proline + H2O + O2 - Bacillus subtilis ? - ? 386435 1.4.3.19 D-proline + H2O + O2 - Geobacillus kaustophilus ? - ? 386435 1.4.3.19 D-proline + H2O + O2 0.3% activity compared to glycine Marinomonas mediterranea ? - ? 386435 1.4.3.19 D-proline + H2O + O2 25% activity compared to glycine Pseudomonas putida ? - ? 386435 1.4.3.19 D-proline + H2O + O2 0.3% activity compared to glycine Marinomonas mediterranea MMB-1 ? - ? 386435 1.4.3.19 D-proline + H2O + O2 25% activity compared to glycine Pseudomonas putida KT 2240 ? - ? 386435 1.4.3.19 D-proline + H2O + O2 - Bacillus cereus ? + NH3 + H2O2 - ? 460970 1.4.3.19 D-proline + H2O + O2 - Bacillus cereus ATCC 14579 ? + NH3 + H2O2 - ? 460970 1.4.3.19 D-Val + H2O + O2 4.8% of the activity with sarcosine Bacillus subtilis ? + NH3 + H2O2 - ? 289406 1.4.3.19 D-Val + H2O + O2 about 35% of the activity with sarcosine Bacillus subtilis 3-methyl-2-oxobutanoate + H2O2 - ? 372984 1.4.3.19 D-valine + H2O + O2 - Geobacillus kaustophilus 3-methyl-2-oxobutanoic acid + NH3 + H2O2 - ? 396948 1.4.3.19 D-valine + H2O + O2 - Geobacillus kaustophilus HTA426 3-methyl-2-oxobutanoic acid + NH3 + H2O2 - ? 396948 1.4.3.19 glycine + H2O + O2 - Bacillus subtilis glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus cereus glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Geobacillus kaustophilus glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Marinomonas mediterranea glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Geobacillus kaustophilus HTA426 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus subtilis glyoxylate + NH3 + H2O2 - r 259018 1.4.3.19 glycine + H2O + O2 - Bacillus licheniformis glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Pseudomonas putida glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Pseudoalteromonas luteoviolacea glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus subtilis glyoxylate + NH3 + H2O2 free glycine oxidase forms the anionic red semiquinone upon photoreduction.This species is thermodynamically stable, as indicated by the large separation of the two single-electron reduction potentials of DeltaE 290 mV. The first potential is pH-independent, while the second is dependent. The midpoint reduction potential exhibits a 23.4 mV/pH unit slope, which is consistent with an overall two-electrons/one-proton transfer in the reduction to yield anionic reduced flavin. In the presence of glycolate and at pH 7.5 the potential for the semiquinone-reduced enzyme couple is shifted positively by about 160 mV, this favors a two-electron transfer compared to the free enzyme. Binding of glycolate and sulfite is also affected by pH ? 259018 1.4.3.19 glycine + H2O + O2 77.4% of the activity with sarcosine Bacillus subtilis glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 100% activity Marinomonas mediterranea glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 as active as sarcosine Bacillus subtilis glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 85% of the activity with sarcosine Bacillus subtilis glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus cereus ATCC 14579 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus subtilis 168 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Pseudoalteromonas luteoviolacea DSM 6061 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus licheniformis J33-8 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 100% activity Marinomonas mediterranea MMB-1 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Pseudomonas putida KT 2240 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + H2O + O2 - Bacillus subtilis R5 glyoxylate + NH3 + H2O2 - r 259018 1.4.3.19 glycine + H2O + O2 - Bacillus cereus HYC-7 glyoxylate + NH3 + H2O2 - ? 259018 1.4.3.19 glycine + O2 - Marinomonas mediterranea 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 - Bacillus subtilis 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 - Bacillus cereus 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 - Geobacillus kaustophilus 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 - Bacillus licheniformis 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 - Bacillus subtilis 168 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 - Bacillus licheniformis J33-8 2-iminoacetate + H2O2 - ? 440256 1.4.3.19 glycine + O2 + H2O - Bacillus subtilis glyoxylate + H2O2 + NH3 - ? 413393 1.4.3.19 glycine + O2 + H2O using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview Bacillus subtilis glyoxylate + H2O2 + NH3 - ? 413393 1.4.3.19 glycine + O2 + H2O - Bacillus subtilis R5 glyoxylate + H2O2 + NH3 - ? 413393 1.4.3.19 glycine + O2 + H2O using samples of [2-RS-3H2,2-14C]-, [2-R-3H,2-14C]-, and [2-S-3H,2-14C]glycine, HSi is removed in the overall process. Incubation of the enzyme with [2-RS-3H2,2-14C]glycine under anaerobic conditions, when only the reducing half of the reaction can occur, leads to the recovery of 98.5% of the original glycine, which has the same 3H:14C ratio as the starting substrate. Isotope effects, overview Bacillus subtilis R5 glyoxylate + H2O2 + NH3 - ? 413393 1.4.3.19 glycine ethyl ester + H2O + O2 30.7% activity compared to glycine Marinomonas mediterranea ? - ? 424821 1.4.3.19 glycine ethyl ester + H2O + O2 5% activity compared to glycine Pseudomonas putida ? - ? 424821 1.4.3.19 glycine ethyl ester + H2O + O2 5% activity compared to glycine Pseudomonas putida KT 2240 ? - ? 424821 1.4.3.19 glycine-ethyl-ester + H2O + O2 - Bacillus subtilis ? - ? 386492 1.4.3.19 glycine-ethyl-ester + H2O + O2 - Geobacillus kaustophilus ? - ? 386492 1.4.3.19 glycine-ethyl-ester + H2O + O2 - Geobacillus kaustophilus HTA426 ? + H2O2 - ? 405316 1.4.3.19 glycine-ethyl-ester + H2O + O2 90% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 405316 1.4.3.19 glycylglycine + H2O + O2 3% activity compared to glycine Pseudomonas putida ? - ? 441426 1.4.3.19 glyphosate + H2O + O2 - Bacillus cereus ? + H2O2 - ? 405336 1.4.3.19 glyphosate + H2O + O2 110% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 405336 1.4.3.19 glyphosate + H2O + O2 - Bacillus cereus ? - ? 440259 1.4.3.19 glyphosate + H2O + O2 - Bacillus licheniformis ? - ? 440259 1.4.3.19 glyphosate + H2O + O2 the wild type enzyme shows very low activity with glyphosate Bacillus cereus ? - ? 440259 1.4.3.19 glyphosate + H2O + O2 - Bacillus licheniformis J33-8 ? - ? 440259 1.4.3.19 glyphosate + H2O + O2 - Bacillus cereus HYC-7 ? - ? 440259 1.4.3.19 glyphosate + H2O + O2 - Bacillus subtilis glyoxylate + aminomethylphosphonic acid + NH3 + H2O2 - ? 441428 1.4.3.19 glyphosate + H2O + O2 - Bacillus subtilis 168 glyoxylate + aminomethylphosphonic acid + NH3 + H2O2 - ? 441428 1.4.3.19 additional information glycine oxidase is converted to a two-electron reduced form upon anaerobic reduction with the individual substrates and its reductive half-reaction is reversible Bacillus subtilis ? - ? 89 1.4.3.19 additional information a general catabolic role of the enzyme on primary or secondary amines is excluded, because the expression of glycine oxidase is not inducible by Gly, sarcosine, or D-Ala as carbon or nitrogen source Bacillus subtilis ? - ? 89 1.4.3.19 additional information the enzyme is strictly stereospecific as it only catalyzes the oxidation of the D-isomer Bacillus subtilis ? - ? 89 1.4.3.19 additional information no activity with butilamine, benzilamine, D-histidine, D-leucine, L-proline, D-tryptophan, and D-tyrosine Geobacillus kaustophilus ? - ? 89 1.4.3.19 additional information substrate preference for amines of small size, like glycine, sarcosine, N-ethyl-glycine, and glycine-ethylester Geobacillus kaustophilus HTA426 ? - ? 89 1.4.3.19 additional information no activity with L-alanine Bacillus subtilis ? - ? 89 1.4.3.19 additional information no activity with L-alanine Bacillus subtilis R5 ? - ? 89 1.4.3.19 N-ethylglycine + H2O + O2 - Bacillus subtilis glyoxylate + ethylamine + H2O2 - ? 289399 1.4.3.19 N-ethylglycine + H2O + O2 - Geobacillus kaustophilus glyoxylate + ethylamine + H2O2 - ? 289399 1.4.3.19 N-ethylglycine + H2O + O2 - Geobacillus kaustophilus HTA426 glyoxylate + ethylamine + H2O2 - ? 289399 1.4.3.19 N-ethylglycine + H2O + O2 85.3% of the activity with sarcosine Bacillus subtilis glyoxylate + ethylamine + H2O2 - ? 289399 1.4.3.19 N-ethylglycine + H2O + O2 about 65% of the activity with sarcosine Bacillus subtilis glyoxylate + ethylamine + H2O2 - ? 289399 1.4.3.19 N-ethylglycine + H2O + O2 120% of the activity with sarcosine Bacillus subtilis glyoxylate + ethylamine + H2O2 - ? 289399 1.4.3.19 N-ethylglycine + H2O + O2 0.3% activity compared to glycine Marinomonas mediterranea ? - ? 424911 1.4.3.19 N-ethylglycine + H2O + O2 40% activity compared to glycine Pseudomonas putida ? - ? 424911 1.4.3.19 N-methyl-D-Ala + H2O + O2 16.9% of the activity with sarcosine Bacillus subtilis ? + NH3 + H2O2 - ? 289405 1.4.3.19 N-methyl-D-Ala + H2O + O2 about 110% of the activity with sarcosine Bacillus subtilis pyruvate + methylamine + H2O2 - ? 373935 1.4.3.19 N-methyl-D-Ala + H2O + O2 75% of the activity with sarcosine Bacillus subtilis ? + H2O2 - ? 406021 1.4.3.19 sarcosine + H2O + O2 - Bacillus subtilis glyoxylate + methylamine + H2O2 - ? 289398 1.4.3.19 sarcosine + H2O + O2 - Geobacillus kaustophilus glyoxylate + methylamine + H2O2 - ? 289398 1.4.3.19 sarcosine + H2O + O2 - Geobacillus kaustophilus HTA426 glyoxylate + methylamine + H2O2 - ? 289398 1.4.3.19 sarcosine + H2O + O2 - Bacillus cereus glyoxylate + methylamine + H2O2 - ? 289398 1.4.3.19 sarcosine + H2O + O2 - Bacillus subtilis 168 glyoxylate + methylamine + H2O2 - ? 289398 1.4.3.19 sarcosine + H2O + O2 - Bacillus cereus ? - ? 370176 1.4.3.19 sarcosine + H2O + O2 - Pseudomonas putida ? - ? 370176 1.4.3.19 sarcosine + H2O + O2 0.1% activity compared to glycine Marinomonas mediterranea ? - ? 370176 1.4.3.19 sarcosine + H2O + O2 0.1% activity compared to glycine Marinomonas mediterranea MMB-1 ? - ? 370176 1.4.3.19 sarcosine + H2O + O2 - Pseudomonas putida KT 2240 ? - ? 370176 1.4.3.19 sarcosine + H2O + O2 - Bacillus cereus HYC-7 ? - ? 370176