1.14.13.195 L-lysine + NADPH + H+ + O2 - Pseudomonas aeruginosa L-lysine N6-oxide + NADP+ + H2O - ? 407250 1.14.13.195 L-lysine + NADPH + H+ + O2 substrate with lower efficiency compared to L-ornithine Aspergillus fumigatus N6-hydroxy-L-lysine + NADP+ + H2O - ? 428733 1.14.13.195 L-lysine + NADPH + H+ + O2 substrate with lower efficiency compared to L-ornithine Aspergillus fumigatus Af293 N6-hydroxy-L-lysine + NADP+ + H2O - ? 428733 1.14.13.195 L-ornithine + NADH + H+ + O2 - Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O - ? 424869 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Pseudomonas fluorescens N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Pseudomonas putida N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Streptomyces coelicolor N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Burkholderia cepacia N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Pseudomonas syringae N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Saccharopolyspora erythraea N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Ralstonia solanacearum N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Pseudomonas sp. N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas fluorescens N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas putida N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Burkholderia cepacia N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas syringae N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Ralstonia solanacearum N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 hydroxylation of the primary amine of ornithine in the initial step of the biosynthesis of siderophore pyoverdin Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 absolutely specific for L-ornithine Streptomyces coelicolor N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 hydroxylation of the primary amine of ornithine Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 no activity with D-ornithine Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 PvdA is highly specific for both substrate and coenzyme, PvdA/FAD forms a ternary complex with NADPH and ornithine for catalysis Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 the enzyme contains the FATGY signature in the putative substrate binding pocket Pseudomonas sp. N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 one of the initial enzymes in the biosynthetic pathway of pyoverdine I the major siderophore produced by Pseudomonas aeruginosa PAO1 to import iron Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 the enzyme is involved in biosynthesis of N5-acetyl-N5-hydroxy-L-ornithine, a building block of the hydroxamate-type siderophore erythrochelin Saccharopolyspora erythraea N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Streptomyces coelicolor A3(2) N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 absolutely specific for L-ornithine Streptomyces coelicolor A3(2) N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Pseudomonas sp. B10 N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 the enzyme contains the FATGY signature in the putative substrate binding pocket Pseudomonas sp. B10 N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 L-ornithine + NADPH + H+ + O2 - Aspergillus fumigatus Af293 N5-hydroxy-L-ornithine + NADP+ + H2O - ? 407252 1.14.13.195 additional information PvdA is the ornithine hydroxylase, which performs the first enzymatic step in preparation of hydroxamate siderophore derivatives, pyoverdin is the hydroxamate siderophore produced by the opportunistic pathogen Pseudomonas aeruginosa under the iron-limiting conditions of the human host, overview. Poor activity with DL-2,3-diaminopropionic acid and D-ornithine, no activity with L-norleucine, 5-aminopentanoic acid, DL-2,4-diaminobutyric acid, and 1,4-diaminobutane, and no activity with NADH as cofactor, substrate specificity, overview Pseudomonas aeruginosa ? - ? 89 1.14.13.195 additional information extending the side chain by one methylene group to L-lysine results in significant NADPH oxidation without formation of the hydroxylated product, indicating that the reaction is uncoupled Pseudomonas aeruginosa ? - ? 89 1.14.13.195 additional information H2O2 formation by NADPH oxidation in the absence of substrate in presence of FAD Pseudomonas aeruginosa ? - ? 89 1.14.13.195 additional information no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview Streptomyces coelicolor ? - ? 89 1.14.13.195 additional information the NADPH oxidase activity of the enzyme is tightly coupled to hydroxylamine formation Pseudomonas aeruginosa ? - ? 89 1.14.13.195 additional information when the enzyme is reduced with NADPH and reacts with molecular oxygen, a C4a-hydroperoxyflavin intermediate is observed. When the enzyme is reduced with NADH, the intermediate is 2fold less stable. Steady-state kinetic isotope effect values for NADPH and NADH are 3 and 2 , respectively, due to differences in the rate of flavin reduction by these coenzymes. NADP+, and not NAD+, protects the enzyme from proteolysis, suggesting that it induces conformational changes upon binding Aspergillus fumigatus ? - ? 89 1.14.13.195 additional information no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview Streptomyces coelicolor A3(2) ? - ? 89