1.1.2.8 1,2-butanediol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460384 1.1.2.8 1,2-cyclopentandiol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460385 1.1.2.8 1,3-butanediol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460386 1.1.2.8 1-butanol + phenazine methosulfate - Pseudomonas putida butanal + reduced phenazine methosulfate - ? 460397 1.1.2.8 1-cyclohexylethanol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460400 1.1.2.8 1-hexanol + phenazine methosulfate - Pseudomonas putida hexanal + reduced phenazine methosulfate - ? 460403 1.1.2.8 1-octanol + phenazine methosulfate - Pseudomonas putida octanal + reduced phenazine methosulfate - ? 460409 1.1.2.8 2,3-butanediol + phenazine methosulfate - Pseudomonas putida ? + reduced phenazine methosulfate - ? 460466 1.1.2.8 2,3-butanediol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460466 1.1.2.8 2,4-pentanediol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460471 1.1.2.8 2-butanol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460486 1.1.2.8 2-butanol + phenazine methosulfate - Pseudomonas putida butan-2-one + reduced phenazine methosulfate - ? 460487 1.1.2.8 2-hexanol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460503 1.1.2.8 2-phenylethanol + phenazine methosulfate - Pseudomonas putida phenylacetaldehyde + reduced phenazine methosulfate - ? 460553 1.1.2.8 2-phenylethanol + phenazine methosulfate converted with 76% of the activity determined with ethanol Pseudomonas putida phenylacetaldehyde + reduced phenazine methosulfate - ? 460553 1.1.2.8 acetaldehyde + phenazine methosulfate - Pseudomonas putida ? + reduced phenazine methosulfate - ? 460755 1.1.2.8 acetaldehyde + phenazine methosulfate converted with 71% of the activity determined with ethanol Pseudomonas putida ? + reduced phenazine methosulfate - ? 460755 1.1.2.8 butanol + 2 cytochrome c - Pseudomonas putida butyraldehyde + 2 reduced cytochrome c - r 440183 1.1.2.8 butanol + 2 cytochrome c - Pseudomonas putida KT 2240 butyraldehyde + 2 reduced cytochrome c - r 440183 1.1.2.8 butyraldehyde + phenazine methosulfate - Pseudomonas putida ? + reduced phenazine methosulfate - ? 460852 1.1.2.8 cinnamyl alcohol + phenazine methosulfate - Pseudomonas putida cinnamyl aldehyde + reduced phenazine methosulfate - ? 460874 1.1.2.8 cis-1,2-cyclohexanediol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460877 1.1.2.8 cyclohexanol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460902 1.1.2.8 D-arabinose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460918 1.1.2.8 D-arabitol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460919 1.1.2.8 D-galactose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460927 1.1.2.8 D-gluconate + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460928 1.1.2.8 D-lyxose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460964 1.1.2.8 D-mannitol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460966 1.1.2.8 D-ribose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460974 1.1.2.8 D-sorbitol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460980 1.1.2.8 D-xylose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 460989 1.1.2.8 diethylstilbestrol + pyrroloquinoline quinone i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol Pseudomonas sp. diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol - ? 440215 1.1.2.8 diethylstilbestrol + pyrroloquinoline quinone i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol Pseudomonas stutzeri diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol - ? 440215 1.1.2.8 diethylstilbestrol + pyrroloquinoline quinone i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol Pseudomonas mendocina diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol - ? 440215 1.1.2.8 diethylstilbestrol + pyrroloquinoline quinone i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol Pseudomonas sp. J51 diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol - ? 440215 1.1.2.8 diethylstilbestrol + pyrroloquinoline quinone i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol Pseudomonas stutzeri ATCC 14405 diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol - ? 440215 1.1.2.8 diethylstilbestrol + pyrroloquinoline quinone i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol Pseudomonas mendocina YMP diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol - ? 440215 1.1.2.8 ethanol + 2 cytochrome c - Pseudomonas putida ethanal + 2 reduced cytochrome c - ? 409371 1.1.2.8 ethanol + 2 cytochrome c EPR-study to elucidate reaction mechanism. In an addition/elimination mechanism, the negatively charged substrate oxygen then performs a nucleophilic addition to the PQQ(C5) to form a covalent substrate-PQQ complex. This is followed by elimination of ethanal, leaving the fully reduced PQQH2. In a hydride transfer mechanism, a nucleophilic addition to the PQQ(C5) again occurs, but this time it is the hydride from C1 of the substrate that is transferred, completing the oxidization of the ethanol to ethanal. Subsequently, the PQQ enolizes to form PQQH2. The results are consistent with either proposed mechanism Pseudomonas aeruginosa ethanal + 2 reduced cytochrome c - ? 409371 1.1.2.8 ethanol + 2 cytochrome c - Pseudomonas putida KT 2240 ethanal + 2 reduced cytochrome c - ? 409371 1.1.2.8 ethanol + 2 cytochrome c - Methylorubrum extorquens acetaldehyde + 2 reduced cytochrome c - r 440233 1.1.2.8 ethanol + 2 cytochrome c - Pseudomonas putida acetaldehyde + 2 reduced cytochrome c - r 440233 1.1.2.8 ethanol + 2 cytochrome c - Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 acetaldehyde + 2 reduced cytochrome c - r 440233 1.1.2.8 ethanol + 2 cytochrome c - Pseudomonas putida KT 2240 acetaldehyde + 2 reduced cytochrome c - r 440233 1.1.2.8 ethanol + 2 ferricytochrome c - Acetobacter pasteurianus ethanal + 2 ferrocytochrome c - ? 424803 1.1.2.8 ethanol + 2 ferricytochrome c - Acetobacter pasteurianus MSU10 ethanal + 2 ferrocytochrome c - ? 424803 1.1.2.8 ethanol + 2 ferricytochrome c - Acetobacter pasteurianus IFO3191 ethanal + 2 ferrocytochrome c - ? 424803 1.1.2.8 ethanol + 2 ferricytochrome c - Acetobacter pasteurianus SKU1108 ethanal + 2 ferrocytochrome c - ? 424803 1.1.2.8 ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine i.e. Wurster's Blue Pseudomonas aeruginosa ethanal + ? - ? 409375 1.1.2.8 ethanol + oxidized 2,6-dichlorophenolindophenol - Pseudomonas aeruginosa ethanal + reduced 2,6-dichlorophenolindophenol - ? 409372 1.1.2.8 ethanol + phenazine methosulfate - Pseudomonas putida acetaldehyde + reduced phenazine methosulfate - ? 439515 1.1.2.8 ethanolamine + phenazine methosulfate - Pseudomonas putida ? + reduced phenazine methosulfate - ? 461038 1.1.2.8 farnesol + phenazine methosulfate - Pseudomonas putida farnesal + reduced phenazine methosulfate - ? 461043 1.1.2.8 glyceraldehyde + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461076 1.1.2.8 glycerol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461082 1.1.2.8 hexanal + phenazine methosulfate - Pseudomonas putida ? + reduced phenazine methosulfate - ? 461108 1.1.2.8 isopropanol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461134 1.1.2.8 L-arabinose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461141 1.1.2.8 L-ribose + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461179 1.1.2.8 meso-erythritol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461209 1.1.2.8 methanol + 2 cytochrome c - Methylorubrum extorquens formaldehyde + 2 reduced cytochrome c - r 440320 1.1.2.8 methanol + 2 cytochrome c the maximal reaction velocity of ExaF-La for formaldehyde is 23% greater than for methanol as the substrate Methylorubrum extorquens formaldehyde + 2 reduced cytochrome c - r 440320 1.1.2.8 methanol + 2 cytochrome c - Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 formaldehyde + 2 reduced cytochrome c - r 440320 1.1.2.8 methanol + 2 cytochrome c the maximal reaction velocity of ExaF-La for formaldehyde is 23% greater than for methanol as the substrate Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 formaldehyde + 2 reduced cytochrome c - r 440320 1.1.2.8 methanol + phenazine methosulfate - Pseudomonas putida formaldehyde + reduced phenazine methosulfate - ? 439741 1.1.2.8 additional information ExaF is functionally an ethanol dehydrogenase, with secondary activities with formaldehyde, methanol, and acetaldehyde. Enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol. Methanol-independent reduction of the latter is not observed with purified enzyme Methylorubrum extorquens ? - ? 89 1.1.2.8 additional information enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida ? - ? 89 1.1.2.8 additional information the enzyme oxidizes a wide variety of secondary alcohols but has higher KM-values than PQQ-dependent glycerol dehydrogenase with regard to linear substrates such as glycerol. Cyclic substrates such as cis-1,2-cyclohexanediol are readily oxidized Gluconobacter sp. CHM43 ? - - 89 1.1.2.8 additional information ExaF is functionally an ethanol dehydrogenase, with secondary activities with formaldehyde, methanol, and acetaldehyde. Enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol. Methanol-independent reduction of the latter is not observed with purified enzyme Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 ? - ? 89 1.1.2.8 additional information enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol Pseudomonas putida KT 2240 ? - ? 89 1.1.2.8 octanal + phenazine methosulfate - Pseudomonas putida ? + reduced phenazine methosulfate - ? 461273 1.1.2.8 propanol + 2 cytochrome c - Pseudomonas putida propanal + 2 reduced cytochrome c - ? 441881 1.1.2.8 propanol + 2 cytochrome c - Pseudomonas putida KT 2240 propanal + 2 reduced cytochrome c - ? 441881 1.1.2.8 ribitol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461341 1.1.2.8 threitol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461374 1.1.2.8 trans-1,2-cyclohexanediol + phenazine methosulfate - Gluconobacter sp. CHM43 ? + reduced phenazine methosulfate - ? 461377