1.1.1.71 additional information lactol substrates (4R,6S)-6-(chloromethyl)oxane-2,4-diol and (4R,6R)-6-butyloxane-2,4-diol for the biocatalytic oxidations are obtained from 2-deoxy-D-ribose 5-phosphate aldolase (DERA) catalyzed reactions of the corresponding acceptor aldehydes chloroacetaldehyde and pentanal, respectively and at least two equivalents of acetaldehyde as described previously. Development of an efficient biocatalytic process on pilot plant scale to oxidize the aliphatic hydroxylactol substrate (4R,6R)-6-butyloxane-2,4-diol to the corresponding lactone product (4R,6R)-6-butyl-4-hydroxyoxan-2-one employing the wild-type alcohol dehydrogenase ADHA Starmerella magnoliae ? - - 89 1.1.1.71 additional information substrate specificity of Ni-PhADH for alcohol oxidation reactions and aldehyde reduction reactions, overview. No activity with 2-butanol, tert-butanol, and 2,3-butanediol Pyrococcus horikoshii ? - - 89 1.1.1.71 additional information lactol substrates (4R,6S)-6-(chloromethyl)oxane-2,4-diol and (4R,6R)-6-butyloxane-2,4-diol for the biocatalytic oxidations are obtained from 2-deoxy-D-ribose 5-phosphate aldolase (DERA) catalyzed reactions of the corresponding acceptor aldehydes chloroacetaldehyde and pentanal, respectively and at least two equivalents of acetaldehyde as described previously. Development of an efficient biocatalytic process on pilot plant scale to oxidize the aliphatic hydroxylactol substrate (4R,6R)-6-butyloxane-2,4-diol to the corresponding lactone product (4R,6R)-6-butyl-4-hydroxyoxan-2-one employing the wild-type alcohol dehydrogenase ADHA Starmerella magnoliae DSMZ 70638 ? - - 89 1.1.1.71 additional information substrate specificity of Ni-PhADH for alcohol oxidation reactions and aldehyde reduction reactions, overview. No activity with 2-butanol, tert-butanol, and 2,3-butanediol Pyrococcus horikoshii DSM 12428 ? - - 89 1.1.1.71 additional information substrate specificity of Ni-PhADH for alcohol oxidation reactions and aldehyde reduction reactions, overview. No activity with 2-butanol, tert-butanol, and 2,3-butanediol Pyrococcus horikoshii NBRC 100139 ? - - 89 1.1.1.71 additional information substrate specificity of Ni-PhADH for alcohol oxidation reactions and aldehyde reduction reactions, overview. No activity with 2-butanol, tert-butanol, and 2,3-butanediol Pyrococcus horikoshii JCM 9974 ? - - 89 1.1.1.71 additional information substrate specificity of Ni-PhADH for alcohol oxidation reactions and aldehyde reduction reactions, overview. No activity with 2-butanol, tert-butanol, and 2,3-butanediol Pyrococcus horikoshii ATCC 700860 ? - - 89 1.1.1.71 additional information substrate specificity of Ni-PhADH for alcohol oxidation reactions and aldehyde reduction reactions, overview. No activity with 2-butanol, tert-butanol, and 2,3-butanediol Pyrococcus horikoshii OT-3 ? - - 89 1.1.1.71 (1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+ - Ralstonia sp. (1R)-1-phenylpropane-1,2-diol + NADP+ - ? 438492 1.1.1.71 (2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+ - Ralstonia sp. (2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+ - ? 438509 1.1.1.71 (2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+ - Ralstonia sp. (2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+ - ? 438520 1.1.1.71 (2S,5S)-2,5-hexanediol + NADP+ - Thermococcus sibiricus ? + NADPH + H+ - ? 427760 1.1.1.71 (2S,5S)-2,5-hexanediol + NADP+ - Thermococcus sibiricus DSM 12597 ? + NADPH + H+ - ? 427760 1.1.1.71 (4R,6R)-6-butyloxane-2,4-diol + NAD+ - Starmerella magnoliae (4R,6R)-6-butyl-4-hydroxyoxan-2-one + NADH + H+ - ? 458762 1.1.1.71 (4R,6R)-6-butyloxane-2,4-diol + NAD+ - Starmerella magnoliae DSMZ 70638 (4R,6R)-6-butyl-4-hydroxyoxan-2-one + NADH + H+ - ? 458762 1.1.1.71 (4R,6R)-6-butyloxane-2,4-diol + NADP+ - Starmerella magnoliae (4R,6R)-6-butyl-4-hydroxyoxan-2-one + NADPH + H+ - ? 458763 1.1.1.71 (4R,6R)-6-butyloxane-2,4-diol + NADP+ - Starmerella magnoliae DSMZ 70638 (4R,6R)-6-butyl-4-hydroxyoxan-2-one + NADPH + H+ - ? 458763 1.1.1.71 (4R,6R)-6-pentyloxane-2,4-diol + NAD+ - Starmerella magnoliae (4R,6R)-4-hydroxy-6-pentyloxan-2-one + NADH + H+ - ? 458764 1.1.1.71 (4R,6R)-6-pentyloxane-2,4-diol + NAD+ - Starmerella magnoliae DSMZ 70638 (4R,6R)-4-hydroxy-6-pentyloxan-2-one + NADH + H+ - ? 458764 1.1.1.71 (4R,6R)-6-pentyloxane-2,4-diol + NADP+ - Starmerella magnoliae (4R,6R)-4-hydroxy-6-pentyloxan-2-one + NADPH + H+ - ? 458765 1.1.1.71 (4R,6R)-6-pentyloxane-2,4-diol + NADP+ - Starmerella magnoliae DSMZ 70638 (4R,6R)-4-hydroxy-6-pentyloxan-2-one + NADPH + H+ - ? 458765 1.1.1.71 (4R,6S)-6-(chloromethyl)oxane-2,4-diol + NAD+ - Starmerella magnoliae (4R,6S)-6-(chloromethyl)-4-hydroxyoxan-2-one + NADH + H+ - ? 458766 1.1.1.71 (4R,6S)-6-(chloromethyl)oxane-2,4-diol + NADP+ - Starmerella magnoliae (4R,6S)-6-(chloromethyl)-4-hydroxyoxan-2-one + NADPH + H+ - ? 458767 1.1.1.71 (RS)-1-phenylethanol + NAD+ 100% activity Thermococcus kodakarensis 1-phenylethanone + NADH + H+ - ? 438032 1.1.1.71 (RS)-1-phenylethanol + NADP+ 100% activity Thermococcus kodakarensis 1-phenylethanone + NADPH + H+ - ? 438033 1.1.1.71 (S)-2-butanol + NADP+ 196% relative activity as compared to 2-propanol Thermococcus sibiricus butanone + NADPH - ? 427803 1.1.1.71 (S)-2-butanol + NADP+ 196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol Thermococcus sibiricus butanone + NADPH + H+ - ? 434959 1.1.1.71 1,3-butanediol + NADP+ 91% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 427812 1.1.1.71 1-(2-bromophenyl)ethanol + NAD(P)+ 11.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(2-bromophenyl)ethanone + NAD(P)H + H+ - ? 438046 1.1.1.71 1-(2-chlorophenyl)ethanol + NAD(P)+ 16.4% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(2-chlorophenyl)ethanone + NAD(P)H + H+ - ? 438047 1.1.1.71 1-(3-bromophenyl)ethanol + NAD(P)+ 315% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(3-bromophenyl)ethanone + NAD(P)H + H+ - ? 438048 1.1.1.71 1-(3-chlorophenyl)ethanol + NAD(P)+ 205% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(3-chlorophenyl)ethanone + NAD(P)H + H+ - ? 438049 1.1.1.71 1-(4-bromophenyl)ethanol + NAD(P)+ 167% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-bromophenyl)ethanone + NAD(P)H + H+ - ? 438050 1.1.1.71 1-(4-chlorophenyl)ethanone + NAD(P)+ 151% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-chlorophenyl)ethanone + NAD(P)H + H+ - ? 438051 1.1.1.71 1-(4-fluorophenyl)ethanol + NAD(P)+ 119% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-fluorophenyl)ethanol + NAD(P)H + H+ - ? 438052 1.1.1.71 1-(4-methylphenyl)ethanone + NAD(P)+ 189% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-(4-methylphenyl)ethanol + NAD(P)H + H+ - ? 438053 1.1.1.71 1-butanol + NAD(P)+ 2.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis butanal + NAD(P)H + H+ - ? 438055 1.1.1.71 1-butanol + NADP+ 80% relative activity as compared to 2-propanol Thermococcus sibiricus butanal + NADPH - ? 371483 1.1.1.71 1-butanol + NADP+ 117% of the activity compared to ethanol Haloarcula marismortui butanal + NADPH + H+ - ? 395234 1.1.1.71 1-butanol + NADP+ 80% compared to activity with 1-propanol Halobacterium salinarum butanal + NADPH + H+ - ? 395234 1.1.1.71 1-butanol + NADP+ 80% of the activtiy with 1-propanol Halobacterium sp. butanal + NADPH + H+ - ? 395234 1.1.1.71 1-butanol + NADP+ 80% compared to activity with 1-propanol Halobacterium salinarum NRC 1 butanal + NADPH + H+ - ? 395234 1.1.1.71 1-butanol + NADP+ 80% of the activtiy with 1-propanol Halobacterium sp. NRC-1 butanal + NADPH + H+ - ? 395234 1.1.1.71 1-butanol + NADP+ - Haloferax volcanii 1-butanal + NADPH + H+ - ? 427829 1.1.1.71 1-heptanol + NAD(P)+ 3.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis heptanal + NAD(P)H + H+ - ? 438057 1.1.1.71 1-hexanol + NAD(P)+ 2.8% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis hexanal + NAD(P)H + H+ - ? 438058 1.1.1.71 1-octanol + NAD(P)+ 2.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis octanal + NAD(P)H + H+ - ? 438059 1.1.1.71 1-pentanol + NAD(P)+ 2.8% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis pentanal + NAD(P)H + H+ - ? 438060 1.1.1.71 1-pentanol + NADP+ 74% of the activity compared to ethanol Haloarcula marismortui pentanal + NADPH + H+ - ? 403283 1.1.1.71 1-pentanol + NADP+ about 75% of the activity with 1-butanol Haloferax volcanii pentanal + NADPH + H+ - ? 403283 1.1.1.71 1-pentanol + NADP+ about 75% of the activity with 1-butanol Haloferax volcanii DSM 3757 pentanal + NADPH + H+ - ? 403283 1.1.1.71 1-phenyl-1,2-ethanediol + NAD(P)+ 3.4% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ? - ? 438061 1.1.1.71 1-phenyl-1-propanol + NAD(P)+ 13.1% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-phenylpropionaldehyde + NAD(P)H + H+ - ? 438062 1.1.1.71 1-phenyl-2-propanol + NAD(P)+ 39.1% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 1-phenylpropan-2-one + NAD(P)H + H+ - ? 438063 1.1.1.71 1-phenylethanol + NADP+ 35% compared to activity with 1-propanol Halobacterium salinarum acetophenone + NADPH + H+ - ? 427836 1.1.1.71 1-phenylethanol + NADP+ 35% of the activtiy with 1-propanol Halobacterium sp. 1-phenylethanone + NADPH + H+ - ? 434987 1.1.1.71 1-phenylmethanol + NADP+ 180% relative activity as compared to 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+ - ? 427837 1.1.1.71 1-propanol + NAD(P)+ 1.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis propionaldehyde + NAD(P)H + H+ - ? 438064 1.1.1.71 1-propanol + NAD+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum propanal + NADH + H+ - ? 383587 1.1.1.71 1-propanol + NAD+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum NRC 1 propanal + NADH + H+ - ? 383587 1.1.1.71 1-propanol + NADP+ - Thermococcus sibiricus propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ - Halobacterium sp. propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ 137% of the activity compared to ethanol Haloarcula marismortui propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ about 60% of the activity with 1-butanol Haloferax volcanii propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ - Halobacterium sp. NRC-1 propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ - Thermococcus sibiricus DSM 12597 propanal + NADPH + H+ - ? 403291 1.1.1.71 1-propanol + NADP+ about 60% of the activity with 1-butanol Haloferax volcanii DSM 3757 propanal + NADPH + H+ - ? 403291 1.1.1.71 2,2,2-trifluoroacetophenone + NAD(P)H + H+ 180% activity compared to acetoin Thermococcus kodakarensis 2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+ - ? 438071 1.1.1.71 2,3-pentanedione + NADPH + H+ - Thermococcus sibiricus ? + NADP+ - ? 187573 1.1.1.71 2,3-pentanedione + NADPH + H+ - Thermococcus sibiricus DSM 12597 ? + NADP+ - ? 187573 1.1.1.71 2-bromoacetophenone + NAD(P)H + H+ 8.7% activity compared to acetoin Thermococcus kodakarensis 1-(2-bromophenyl)ethanone + NAD(P)+ - ? 438079 1.1.1.71 2-bromobenzaldehyde + NADPH + H+ - Ralstonia sp. 2-bromobenzyl alcohol + NADP+ - ? 438710 1.1.1.71 2-butanol + NAD(P)+ 22.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis butan-2-one + NAD(P)H + H+ - ? 438080 1.1.1.71 2-butanol + NADP+ 43% of the activity compared to ethanol Haloarcula marismortui 2-butanone + NADPH + H+ - ? 422388 1.1.1.71 2-butanol + NADP+ 30% compared to activity with 1-propanol Halobacterium salinarum butanone + NADPH + H+ - ? 427897 1.1.1.71 2-butanol + NADP+ about 25% of the activity with 1-butanol Haloferax volcanii butanone + NADPH + H+ - ? 427897 1.1.1.71 2-butanol + NADP+ 30% of the activtiy with 1-propanol Halobacterium sp. butanone + NADPH + H+ - ? 427897 1.1.1.71 2-chloro-1-phenylethanol + NAD(P)+ 0.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 2-chloro-1-phenylethan-1-one + NAD(P)H + H+ - ? 438081 1.1.1.71 2-fluorobenzaldehyde + NADPH + H+ - Ralstonia sp. 2-fluorobenzyl alcohol + NADP+ - ? 438726 1.1.1.71 2-heptanol + NAD(P)+ 61.6% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis heptan-2-one + NAD(P)H + H+ - ? 438083 1.1.1.71 2-hexanol + NAD(P)+ 38.7% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis hexan-2-one + NAD(P)H + H+ - ? 438084 1.1.1.71 2-hexanone + NAD(P)H + H+ 3.6% activity compared to acetoin Thermococcus kodakarensis hexan-2-ol + NAD(P)+ - ? 438085 1.1.1.71 2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+ - Ralstonia sp. 2-methyl-1-phenylpropan-1,2-diol + NADP+ - ? 438731 1.1.1.71 2-hydroxy-3-methoxybenzaldehyde + NADPH + H+ - Solanum tuberosum 2-hydroxy-3-methoxybenzyl alcohol + NADP+ - ? 371682 1.1.1.71 2-methoxybenzaldehyde + NADPH + H+ - Ralstonia sp. 2-methoxybenzyl alcohol + NADP+ - ? 187529 1.1.1.71 2-methoxybenzaldehyde + NADPH + H+ highest activity Solanum tuberosum 2-methoxybenzyl alcohol + NADP+ - ? 187529 1.1.1.71 2-methoxybenzaldehyde + NADPH + H+ - Ralstonia sp. DSM 6428 2-methoxybenzyl alcohol + NADP+ - ? 187529 1.1.1.71 2-methylbenzaldehyde + NADPH + H+ - Ralstonia sp. 2-methylbenzyl alcohol + NADP+ - ? 438734 1.1.1.71 2-octanol + NAD(P)+ 45.8% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis octan-2-one + NAD(P)H + H+ - ? 438091 1.1.1.71 2-pentanol + NAD(P)+ 30.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis pentan-2-one + NAD(P)H + H+ - ? 438098 1.1.1.71 2-pentanol + NADP+ 67% relative activity as compared to 2-propanol Thermococcus sibiricus 2-pentanone + NADPH + H+ - ? 422467 1.1.1.71 2-pentanol + NADP+ 67% of the activity with 2-propanol Thermococcus sibiricus pentan-2-one + NADPH + H+ - ? 435063 1.1.1.71 2-phenylethanol + NAD(P)+ 0.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis phenylacetaldehyde + NAD(P)H + H+ - ? 438099 1.1.1.71 2-propanol + NAD(P)+ 5.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis propan-2-one + NAD(P)H + H+ - ? 438100 1.1.1.71 2-propanol + NADP+ - Thermococcus sibiricus acetone + NADPH - ? 371771 1.1.1.71 2-propanol + NADP+ - Thermococcus sibiricus DSM 12597 acetone + NADPH - ? 371771 1.1.1.71 2-propanol + NADP+ - Thermococcus sibiricus acetone + NADPH + H+ - ? 383788 1.1.1.71 2-propanol + NADP+ 25% compared to activity with 1-propanol Halobacterium salinarum acetone + NADPH + H+ - ? 383788 1.1.1.71 2-propanol + NADP+ 25% compared to activity with 1-propanol Halobacterium salinarum NRC 1 acetone + NADPH + H+ - ? 383788 1.1.1.71 2-propanol + NADP+ - Thermococcus sibiricus DSM 12597 acetone + NADPH + H+ - ? 383788 1.1.1.71 3,4-dimethoxybenzaldehyde + NADPH + H+ - Solanum tuberosum 3,4-dimethoxybenzyl alcohol + NADP+ - ? 371797 1.1.1.71 3-hydroxypropionaldehyde + NADPH + H+ 100% activity Klebsiella pneumoniae propan-1,3-diol + NADP+ - ? 438788 1.1.1.71 3-hydroxypropionaldehyde + NADPH + H+ 100% activity Klebsiella pneumoniae DSM2026 propan-1,3-diol + NADP+ - ? 438788 1.1.1.71 3-methoxybenzaldehyde + NADPH + H+ - Ralstonia sp. 3-methoxybenzyl alcohol + NADP+ - ? 438791 1.1.1.71 3-methyl-2-pentanone + NADPH + H+ 13% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+ - ? 427985 1.1.1.71 3-methylbenzaldehyde + NADPH + H+ - Ralstonia sp. 3-methylbenzyl alcohol + NADP+ - ? 438797 1.1.1.71 3-methylbutanal + NADP+ about% of the activity with 1-butanol Haloferax volcanii ? + NADPH + H+ - ? 427988 1.1.1.71 4-androstene-3,17-dione + NADPH + H+ - Homo sapiens ? - ? 425619 1.1.1.71 4-bromobenzaldehyde + NADPH + H+ - Ralstonia sp. 4-bromobenzyl alcohol + NADP+ - ? 438827 1.1.1.71 4-fluorobenzaldehyde + NADPH + H+ - Ralstonia sp. 4-fluorobenzyl alcohol + NADP+ - ? 438839 1.1.1.71 4-methoxybenzaldehyde + NADPH + H+ - Ralstonia sp. 4-methoxybenzyl alcohol + NADP+ - ? 257303 1.1.1.71 4-methoxybenzaldehyde + NADPH + H+ - Solanum tuberosum 4-methoxybenzyl alcohol + NADP+ - ? 257303 1.1.1.71 4-methoxybenzaldehyde + NADPH + H+ - Ralstonia sp. DSM 6428 4-methoxybenzyl alcohol + NADP+ - ? 257303 1.1.1.71 4-methylbenzaldehyde + NADPH + H+ - Ralstonia sp. 4-methylbenzyl alcohol + NADP+ - ? 438848 1.1.1.71 4-methylnitrosamino-1-(3-pyridyl)-1-butanone + NADPH + H+ - Homo sapiens ? - ? 438849 1.1.1.71 4-methylpentan-2-one + NADPH + H+ - Ralstonia sp. 4-methylpentan-2-ol + NADP+ - ? 438850 1.1.1.71 4-nitroacetophenone + NADPH + H+ - Homo sapiens ? - ? 438856 1.1.1.71 acetaldehyde + NADH + H+ - Acetivibrio thermocellus ethanol + NAD+ - ? 139564 1.1.1.71 acetaldehyde + NADH + H+ - Thermoanaerobacterium saccharolyticum ethanol + NAD+ - ? 139564 1.1.1.71 acetaldehyde + NADPH + H+ - Haloarcula marismortui ethanol + NADP+ - ? 139551 1.1.1.71 acetaldehyde + NADPH + H+ - Halobacterium sp. ethanol + NADP+ - ? 139551 1.1.1.71 acetaldehyde + NADPH + H+ - Acetivibrio thermocellus ethanol + NADP+ - ? 139551 1.1.1.71 acetaldehyde + NADPH + H+ - Thermoanaerobacterium saccharolyticum ethanol + NADP+ - ? 139551 1.1.1.71 acetaldehyde + NADPH + H+ 2.2% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae ethanol + NADP+ - ? 139551 1.1.1.71 acetaldehyde + NADPH + H+ 2.2% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae DSM2026 ethanol + NADP+ - ? 139551 1.1.1.71 acetaldehyde + NADPH + H+ - Halobacterium sp. NRC-1 ethanol + NADP+ - ? 139551 1.1.1.71 acetohexamide + NADPH + H+ - Homo sapiens ? - ? 384102 1.1.1.71 acetoin + NAD(P)+ 100% activity Thermococcus kodakarensis butan-2,3-dione + NAD(P)H + H+ - ? 438129 1.1.1.71 acetoin + NAD(P)H + H+ 4.5% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis 2,3-butanediol + NAD(P)+ - ? 188668 1.1.1.71 acetoin + NADPH + H+ 1.9% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae ? - ? 439012 1.1.1.71 acetone + NAD(P)H + H+ 4.1% activity compared to acetoin Thermococcus kodakarensis propan-2-ol + NAD(P)+ - ? 438130 1.1.1.71 acrolein + NADPH + H+ 41.8% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae prop-2-en-1-ol + NADP+ - ? 439096 1.1.1.71 all-trans-retinal + NADPH + H+ - Homo sapiens all-trans-retinol + NADP+ - ? 187638 1.1.1.71 benzaldehyde + NAD(P)H - Rattus norvegicus benzyl alcohol + NAD(P)+ - ? 165076 1.1.1.71 benzaldehyde + NADPH + H+ - Solanum tuberosum benzyl alcohol + NADP+ - ? 372517 1.1.1.71 benzyl alcohol + NADP+ 20% compared to activity with 1-propanol Halobacterium salinarum benzaldehyde + NADPH + H+ - ? 404469 1.1.1.71 benzyl alcohol + NADP+ about% of the activity with 1-butanol Haloferax volcanii benzaldehyde + NADPH + H+ - ? 404469 1.1.1.71 benzyl alcohol + NADP+ 180% of the activity with 2-propanol Thermococcus sibiricus benzaldehyde + NADPH + H+ - ? 404469 1.1.1.71 benzyl alcohol + NADP+ about% of the activity with 1-butanol Haloferax volcanii DSM 3757 benzaldehyde + NADPH + H+ - ? 404469 1.1.1.71 butan-2-ol + NADP+ - Ralstonia sp. butan-2-one + NADPH + H+ - ? 439272 1.1.1.71 butan-2-one + NADPH + H+ - Ralstonia sp. butan-2-ol + NADP+ - ? 439274 1.1.1.71 butanal + NAD(P)H + H+ - Rattus norvegicus butan-1-ol + NAD(P)+ - ? 188581 1.1.1.71 butanal + NADPH + H+ 44% activity compared to pentanal Pectobacterium atrosepticum 1-butanol + NAD(P)+ - ? 439276 1.1.1.71 butyraldehyde + NADPH + H+ 118.4% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae butanol + NADP+ - ? 384385 1.1.1.71 butyraldehyde + NADPH + H+ 118.4% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae DSM2026 butanol + NADP+ - ? 384385 1.1.1.71 cellobiose + NADP+ 71% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428479 1.1.1.71 cellobiose + NADP+ 71% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428479 1.1.1.71 cinnamaldehyde + NADPH + H+ - Solanum tuberosum cinnamyl alcohol + NADP+ - ? 372712 1.1.1.71 cis-2-butenol + NAD(P)H - Leuconostoc mesenteroides trans-2-butenal + NAD(P)+ - ? 188577 1.1.1.71 cis-2-butenol + NAD(P)H - Leuconostoc mesenteroides IFO 3426 trans-2-butenal + NAD(P)+ - ? 188577 1.1.1.71 cis-4-cyclopentene-1,3-diol + NADP+ - Ralstonia sp. (R)-4-hydroxy-2-cyclopentenone + NADPH + H+ - ? 439341 1.1.1.71 cis-4-heptenal + NAD(P)+ - Leuconostoc mesenteroides cis-4-heptenol + NAD(P)H - ? 188579 1.1.1.71 citral + NAD(P)H - Rattus norvegicus citrol + NAD(P)+ - ? 188588 1.1.1.71 cyclohexanol + NAD(P)+ 52.3% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis cyclohexanone + NAD(P)H + H+ - ? 438296 1.1.1.71 cyclohexanone + NAD(P)H + H+ 19.1% activity compared to acetoin Thermococcus kodakarensis cyclohexanol + NAD(P)+ - ? 438297 1.1.1.71 D-arabinose + NAD(P)+ 21.4% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ? - ? 438301 1.1.1.71 D-arabinose + NADP+ 200% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428516 1.1.1.71 D-arabinose + NADP+ 200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose Thermococcus sibiricus D-arabitol + NADPH + H+ - ? 435425 1.1.1.71 D-glucose + NADP+ 146% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 405285 1.1.1.71 D-glucose + NADP+ 146% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 405285 1.1.1.71 D-mannose + NADP+ 48% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428531 1.1.1.71 D-ribose + NADP+ 35% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428534 1.1.1.71 D-xylose + NADP+ 246% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428545 1.1.1.71 D-xylose + NADP+ 246% of the activity with 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428545 1.1.1.71 decanal + NAD(P)H - Rattus norvegicus decan-1-ol + NAD(P)+ - ? 188584 1.1.1.71 dihydroxyacetone + NAD(P)H + H+ 34.8% activity compared to acetoin Thermococcus kodakarensis ? - ? 438320 1.1.1.71 dimethylglyoxal + NADPH + H+ 270% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+ - ? 428592 1.1.1.71 dimethylglyoxal + NADPH + H+ 270% of the activity with pyruvaldehyde Thermococcus sibiricus ? + NADP+ - ? 428592 1.1.1.71 DL-glyceraldehyde + NADPH + H+ - Homo sapiens ? - ? 426340 1.1.1.71 dodecanal + NAD(P)H - Rattus norvegicus dodecan-1-ol + NAD(P)+ - ? 188585 1.1.1.71 estrone + NADPH + H+ - Homo sapiens ? - ? 439513 1.1.1.71 ethanal + NAD(P)H - Rattus norvegicus ethanol + NAD(P)+ - ? 188557 1.1.1.71 ethanediol + NADP+ - Moniezia expansa glyoxal + NADPH - ? 188559 1.1.1.71 ethanol + NAD(P)+ 0.5% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis acetaldehyde + NAD(P)H + H+ - ? 438333 1.1.1.71 ethanol + NAD+ enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+ Haloarcula marismortui acetaldehyde + NADH + H+ - ? 384669 1.1.1.71 ethanol + NADP+ enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+ Haloarcula marismortui acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ 36% relative activity as compared to 2-propanol Thermococcus sibiricus acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ 85% compared to activity with 1-propanol Halobacterium salinarum acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ about 50% of the activity with 1-butanol Haloferax volcanii acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ 84% of the activtiy with 1-propanol Halobacterium sp. acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ 85% compared to activity with 1-propanol Halobacterium salinarum NRC 1 acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ 84% of the activtiy with 1-propanol Halobacterium sp. NRC-1 acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethanol + NADP+ about 50% of the activity with 1-butanol Haloferax volcanii DSM 3757 acetaldehyde + NADPH + H+ - ? 187591 1.1.1.71 ethyl pyruvate + NAD(P)H + H+ 294% activity compared to acetoin Thermococcus kodakarensis ? - ? 438334 1.1.1.71 farnesyl aldehyde + NAD(P)H - Rattus norvegicus farnesol + NAD(P)+ - ? 188590 1.1.1.71 formaldehyde + NADPH + H+ - Ralstonia sp. methanol + NADP+ - ? 437552 1.1.1.71 furan-2-carbaldehyde + NADPH + H+ - Ralstonia sp. (furan-2-yl)methanol + NADP+ - ? 439532 1.1.1.71 furfural + NADPH + H+ 5% activity compared to pentanal Pectobacterium atrosepticum (furan-2-yl)methanol + NAD(P)+ - ? 439533 1.1.1.71 glycerol + NADP+ 16% relative activity as compared to 2-propanol Thermococcus sibiricus glyceraldehyde + NADPH + H+ - ? 414663 1.1.1.71 glycoaldehyde + NADPH + H+ 5% activity compared to pentanal Pectobacterium atrosepticum ? - ? 439583 1.1.1.71 glyoxylic acid + NADPH + H+ 36% relative activity as compared to pyruvaldehyde Thermococcus sibiricus ? + NADP+ - ? 428660 1.1.1.71 heptan-4-one + NADPH + H+ - Ralstonia sp. heptan-4-ol + NADP+ - ? 439601 1.1.1.71 heptanal + NAD(P)H - Rattus norvegicus heptan-1-ol + NAD(P)+ - ? 188582 1.1.1.71 heptanal + NADPH + H+ 56% activity compared to pentanal Pectobacterium atrosepticum 1-heptanol + NAD(P)+ - ? 439602 1.1.1.71 hexan-1-ol + NADP+ - Bacillus subtilis hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Escherichia coli hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Rhodococcus rhodochrous hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Pseudomonas aeruginosa hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Pseudomonas putida hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Acinetobacter calcoaceticus hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Mycobacterium tuberculosis hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Rhodotorula graminis hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Streptomyces rimosus hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Moniezia expansa hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Streptomyces rimosus 4018 hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Rhodococcus rhodochrous NCIMB 13259 hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Pseudomonas putida NCIMB 9494 hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Rhodotorula graminis KGX 39 hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Bacillus subtilis NCIMB 3610 hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Escherichia coli ML30 hexanal + NADPH - ? 188556 1.1.1.71 hexan-1-ol + NADP+ - Ralstonia sp. hexaldehyde + NADPH + H+ - ? 439603 1.1.1.71 hexan-2-one + NADPH + H+ - Ralstonia sp. hexan-2-ol + NADP+ - ? 439604 1.1.1.71 hexanal + NAD(P)H + H+ 12.9% activity compared to acetoin Thermococcus kodakarensis hexan-1-ol + NAD(P)+ - ? 438358 1.1.1.71 hexanal + NADPH + H+ - Solanum tuberosum hexanol + NADP+ - ? 165073 1.1.1.71 hexanal + NADPH + H+ - Ralstonia sp. hexan-1-ol + NADP+ - ? 437607 1.1.1.71 hexanal + NADPH + H+ 62% activity compared to pentanal Pectobacterium atrosepticum 1-hexanol + NAD(P)+ - ? 439605 1.1.1.71 hydrocinnamaldehyde + NADPH + H+ - Solanum tuberosum hydrocinnamyl alcohol + NADP+ - ? 373439 1.1.1.71 isoamyl alcohol + NADP+ about 80% of the activity with 1-butanol Haloferax volcanii ? + NADPH + H+ - ? 428710 1.1.1.71 L-arabinose + NAD(P)+ 5.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ? - ? 373545 1.1.1.71 L-arabinose + NADP+ 17% relative activity as compared to 2-propanol Thermococcus sibiricus ? + NADPH + H+ - ? 428721 1.1.1.71 linolenyl aldehyde + NAD(P)H - Rattus norvegicus linolenol + NAD(P)+ - ? 188589 1.1.1.71 linoleyl aldehyde + NAD(P)H - Rattus norvegicus linoleol + NAD(P)+ - ? 188591 1.1.1.71 meso-2,3-butanediol + NAD+ 272% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ? - ? 438394 1.1.1.71 meso-2,3-butanediol + NADP+ 272% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis ? - ? 438395 1.1.1.71 methanol + NAD(P)+ 0.2% activity compared to (RS)-1-phenylethanol Thermococcus kodakarensis formaldehyde + NAD(P)H + H+ - ? 438396 1.1.1.71 methyl benzoylformate + NAD(P)H + H+ 67% activity compared to acetoin Thermococcus kodakarensis ? - ? 438397 1.1.1.71 methylglyoxal + NADPH + H+ - Moniezia expansa 2-hydroxy-propanal + NADP+ - ? 188576 1.1.1.71 methylglyoxal + NADPH + H+ 29% activity compared to pentanal Pectobacterium atrosepticum ? - ? 405858 1.1.1.71 additional information the enzyme plays an important role in potato defence response to Erwinia carotovora Solanum tuberosum ? - ? 89 1.1.1.71 additional information not: conferyl aldehyde and sinapaldehyde, the enzyme exhibits an absolute requirement for NADPH as cofactor and can not be substituted by NADH Solanum tuberosum ? - ? 89 1.1.1.71 additional information the enzyme does not accept methanol, 2-propanol, glycerol or glucose as substrates Haloarcula marismortui ? - ? 89 1.1.1.71 additional information HvADH2 accepts a broad range of substrates, no activity with methanol Haloferax volcanii ? - ? 89 1.1.1.71 additional information no oxidation of methanol, methoxyethanol, ethylene glycol, or D-galactose. No reduction of acetone, cyclopentanone, D-arabinose, D-xylose, D-glucose or cellobiose Thermococcus sibiricus ? - ? 89 1.1.1.71 additional information preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol Halobacterium salinarum ? - ? 89 1.1.1.71 additional information no substrates: methanol, glycerol Halobacterium sp. ? - ? 89 1.1.1.71 additional information in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. In high-ethanol-producing (ethanologen) strains of Thermoanaerobacterium saccharolyticum, both ALDH and ADH activities show increased NADPH-linked activity Thermoanaerobacterium saccharolyticum ? - ? 89 1.1.1.71 additional information in wild-type strains, NADH is the preferred cofactor for both aldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH activities. The AdhE protein of the ethanologenic strain of Clostridium thermocellum has acquired high NADPH-linked ADH activity while maintaining NADH-linked ALDH and ADH activities at wild-type levels Acetivibrio thermocellus ? - ? 89 1.1.1.71 additional information no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone Thermococcus kodakarensis ? - ? 89 1.1.1.71 additional information 4-nitroacetophenone, benzyl, cortisone and progesterone are no substrates Homo sapiens ? - ? 89 1.1.1.71 additional information no activity with dihydroxyacetone, hydroxyacetone, and acetone Klebsiella pneumoniae ? - ? 89 1.1.1.71 additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum ? - ? 89 1.1.1.71 additional information no activity with methanol and ethanol Ralstonia sp. ? - ? 89 1.1.1.71 additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum ? - ? 89 1.1.1.71 additional information preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol Halobacterium salinarum NRC 1 ? - ? 89 1.1.1.71 additional information no activity with methanol and ethanol Ralstonia sp. DSM 6428 ? - ? 89 1.1.1.71 additional information no activity with dihydroxyacetone, hydroxyacetone, and acetone Klebsiella pneumoniae DSM2026 ? - ? 89 1.1.1.71 additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum DSM18077 ? - ? 89 1.1.1.71 additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum DSM18077 ? - ? 89 1.1.1.71 additional information no substrates: methanol, glycerol Halobacterium sp. NRC-1 ? - ? 89 1.1.1.71 additional information HvADH2 accepts a broad range of substrates, no activity with methanol Haloferax volcanii DSM 3757 ? - ? 89 1.1.1.71 additional information no activity with glyoxal, methanal, and ethanal Pectobacterium atrosepticum DSM 18077 ? - ? 89 1.1.1.71 additional information the enzyme shows no oxidative activities towards alcoholic compounds Pectobacterium atrosepticum DSM 18077 ? - ? 89 1.1.1.71 octan-1-ol + NADP+ - Ralstonia sp. octaldehyde + NADPH + H+ - ? 439797 1.1.1.71 octan-2-one + NADPH + H+ - Ralstonia sp. octan-2-ol + NADP+ - ? 439799 1.1.1.71 octanal + NAD(P)H + H+ - Rattus norvegicus octan-1-ol + NAD(P)+ - ? 188583 1.1.1.71 octanal + NADPH + H+ - Solanum tuberosum octanol + NADP+ - ? 374084 1.1.1.71 octanal + NADPH + H+ 55% activity compared to pentanal Pectobacterium atrosepticum 1-octanol + NAD(P)+ - ? 439800 1.1.1.71 oleyl aldehyde + NAD(P)H - Rattus norvegicus oleol + NAD(P)+ - ? 188592 1.1.1.71 pentan-1-ol + NADP+ - Saccharomyces cerevisiae pentanal + NADPH - ? 188564 1.1.1.71 pentan-1-ol + NADP+ - Saccharomyces cerevisiae D273-10B pentanal + NADPH - ? 188564 1.1.1.71 pentan-1-ol + NADP+ - Ralstonia sp. pentaldehyde + NADPH + H+ - ? 439812 1.1.1.71 pentanal + NADPH + H+ 100% activity Pectobacterium atrosepticum 1-pentanol + NAD(P)+ - ? 439813 1.1.1.71 phenylacetaldehyde + NAD(P)H + H+ 18.6% activity compared to acetoin Thermococcus kodakarensis 2-phenylethan-1-ol + NAD(P)+ - ? 438422 1.1.1.71 propan-1-ol + NADP+ - Ralstonia sp. propionaldehyde + NADPH + H+ - ? 439838 1.1.1.71 propan-2-ol + NADP+ - Ralstonia sp. propan-2-one + NADPH + H+ - ? 439840 1.1.1.71 propanal + NADPH + H+ 14% activity compared to pentanal Pectobacterium atrosepticum 1-propanol + NAD(P)+ - ? 439843 1.1.1.71 propionaldehyde + NADPH + H+ 37.1% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae propanol + NADP+ - ? 406481 1.1.1.71 propionaldehyde + NADPH + H+ 37.1% activity compared to 3-hydroxypropionaldehyde Klebsiella pneumoniae DSM2026 propanol + NADP+ - ? 406481 1.1.1.71 pyridine-2-carbaldehyde + NADPH + H+ - Ralstonia sp. (pyridin-2-yl)methanol + NADP+ - ? 439854 1.1.1.71 pyruvaldehyde + NADPH + H+ - Thermococcus sibiricus lactaldehyde + NADP+ - ? 423978 1.1.1.71 pyruvaldehyde + NADPH + H+ - Thermococcus sibiricus DSM 12597 lactaldehyde + NADP+ - ? 423978 1.1.1.71 pyruvaldehyde + NADPH + H+ - Thermococcus sibiricus ? + NADP+ - ? 428901 1.1.1.71 pyruvic aldehyde + NAD(P)H + H+ 328% activity compared to acetoin Thermococcus kodakarensis ? - ? 438436 1.1.1.71 rac-1-nonen-4-ol + NADP+ - Thermoanaerobacter brockii (S)-1-nonen-4-ol + 1-nonen-4-one + NADPH + H+ - ? 439866 1.1.1.71 rac-1-phenylethanol + NAD(P)+ - Rhodococcus ruber acetophenone + (R)-1-phenylethanol + NAD(P)H + H+ - ? 439867 1.1.1.71 rac-1-phenylethanol + NADP+ - Thermoanaerobacter brockii (R)-1-phenylethanol + (S)-1-phenylethanol + NADPH + H+ - ? 439868 1.1.1.71 retinal + NAD(P)H - Rattus norvegicus retinol + NAD(P)+ - ? 188554 1.1.1.71 retinaldehyde + NADH - Leuconostoc mesenteroides retinol + NAD+ - ? 383355 1.1.1.71 retinaldehyde + NADH - Cucumis melo retinol + NAD+ - ? 383355 1.1.1.71 retinaldehyde + NADH - Homo sapiens retinol + NAD+ - ? 383355 1.1.1.71 salicylaldehye + NADPH + H+ - Solanum tuberosum salicylalcohol + NADP+ - ? 374623 1.1.1.71 tetradecanal + NAD(P)H - Rattus norvegicus tetradecan-1-ol + NAD(P)+ - ? 188586 1.1.1.71 trans-2,cis-6-nonadienal + NAD(P)+ - Leuconostoc mesenteroides trans-2,cis-6-nonadienol + NAD(P)H - ? 188580 1.1.1.71 trans-2,cis-6-nonadienal + NADPH + H+ - Solanum tuberosum trans-2,cis-6-nonadienol + NADP+ - ? 374729 1.1.1.71 trans-2-hexenal + NADPH + H+ - Solanum tuberosum trans-2-hexenol + NADP+ - ? 374735 1.1.1.71 trans-2-nonenal + NADPH + H+ - Moniezia expansa trans-2-nonen-1-ol + NADP+ - ? 188573 1.1.1.71 trans-2-nonenal + NADPH + H+ - Solanum tuberosum trans-2-nonenol + NADP+ - ? 374738 1.1.1.71 trans-2-octenal + NADPH + H+ - Moniezia expansa trans-2-octen-1-ol + NADP+ - ? 188574 1.1.1.71 trans-2-pentenol + NAD(P)+ - Leuconostoc mesenteroides trans-2-pentenal + NAD(P)H - ? 188578 1.1.1.71 trans-trans-2,4-decadienal + NADPH + H+ - Moniezia expansa trans-trans-2,4-decadienol + NADP+ - ? 188575 1.1.1.71 vanillyl alcohol + NAD+ - Equus caballus vanillin + NADH + H+ - ? 439983 1.1.1.71 (R)-2-hydroxypropiophenone + NADPH + H+ - Ralstonia sp. ? - r 438556 1.1.1.71 (S)-2-hydroxypropiophenone + NADPH + H+ - Ralstonia sp. ? - r 438590 1.1.1.71 1,2-propanediol + NAD+ - Pyrococcus horikoshii ? + NADH + H+ - r 422248 1.1.1.71 1,3-propanediol + NAD+ - Pyrococcus horikoshii ? + NADH + H+ - r 458819 1.1.1.71 1,4-butanediol + NAD+ - Pyrococcus horikoshii ? + NADH + H+ - r 440481 1.1.1.71 1-butanol + NAD+ - Pyrococcus horikoshii butanal + NADH + H+ - r 383560 1.1.1.71 1-butanol + NAD+ - Pyrococcus horikoshii DSM 12428 butanal + NADH + H+ - r 383560 1.1.1.71 1-butanol + NAD+ - Pyrococcus horikoshii NBRC 100139 butanal + NADH + H+ - r 383560 1.1.1.71 1-butanol + NAD+ - Pyrococcus horikoshii JCM 9974 butanal + NADH + H+ - r 383560 1.1.1.71 1-butanol + NAD+ - Pyrococcus horikoshii ATCC 700860 butanal + NADH + H+ - r 383560 1.1.1.71 1-butanol + NAD+ - Pyrococcus horikoshii OT-3 butanal + NADH + H+ - r 383560 1.1.1.71 1-butanol + NADP+ - Pyrococcus horikoshii butanal + NADPH + H+ - r 395234 1.1.1.71 1-heptanol + NAD+ - Pyrococcus horikoshii heptanal + NADH + H+ - r 383568 1.1.1.71 1-heptanol + NAD+ - Pyrococcus horikoshii DSM 12428 heptanal + NADH + H+ - r 383568 1.1.1.71 1-heptanol + NAD+ - Pyrococcus horikoshii NBRC 100139 heptanal + NADH + H+ - r 383568 1.1.1.71 1-heptanol + NAD+ - Pyrococcus horikoshii JCM 9974 heptanal + NADH + H+ - r 383568 1.1.1.71 1-heptanol + NAD+ - Pyrococcus horikoshii ATCC 700860 heptanal + NADH + H+ - r 383568 1.1.1.71 1-heptanol + NAD+ - Pyrococcus horikoshii OT-3 heptanal + NADH + H+ - r 383568 1.1.1.71 1-hexanol + NAD+ - Pyrococcus horikoshii hexanal + NADH + H+ - r 383570 1.1.1.71 1-hexanol + NAD+ - Pyrococcus horikoshii DSM 12428 hexanal + NADH + H+ - r 383570 1.1.1.71 1-hexanol + NAD+ - Pyrococcus horikoshii NBRC 100139 hexanal + NADH + H+ - r 383570 1.1.1.71 1-hexanol + NAD+ - Pyrococcus horikoshii JCM 9974 hexanal + NADH + H+ - r 383570 1.1.1.71 1-hexanol + NAD+ - Pyrococcus horikoshii ATCC 700860 hexanal + NADH + H+ - r 383570 1.1.1.71 1-hexanol + NAD+ - Pyrococcus horikoshii OT-3 hexanal + NADH + H+ - r 383570 1.1.1.71 1-octanol + NAD+ - Pyrococcus horikoshii octanal + NADH + H+ - r 383576 1.1.1.71 1-pentanol + NAD+ - Pyrococcus horikoshii pentanal + NADH + H+ - r 383578 1.1.1.71 1-propanol + NAD+ - Pyrococcus horikoshii propanal + NADH + H+ - r 383587 1.1.1.71 2,5-hexandione + NADH + H+ - Ralstonia sp. ? - r 438704 1.1.1.71 2-bromobenzaldehyde + NADPH - Ralstonia sp. 2-bromobenzyl alcohol + NADP+ - r 438711 1.1.1.71 2-cyclohexenol + NADP+ - Ralstonia sp. 2-cyclohexenone + NADH + H+ - r 439370 1.1.1.71 2-cyclohexenone + NADH + H+ - Ralstonia sp. 2-cyclohexenol + NADP+ - r 439371 1.1.1.71 3-fluorobenzaldehyde + NADPH - Ralstonia sp. 3-fluorobenzyl alcohol + NADP+ - r 425552 1.1.1.71 3-methylcyclohexanol + NADP+ - Ralstonia sp. ? - r 438798 1.1.1.71 3-methylcyclohexanone + NADH + H+ - Ralstonia sp. 3-methylcyclohexanol + NAD+ - r 419997 1.1.1.71 4-phenyl-2-butanone + NADH + H+ - Ralstonia sp. 4-phenyl-2-butanol + NAD+ - r 438864 1.1.1.71 acetaldehyde + NADH + H+ - Cucumis melo ethanol + NAD+ - r 139564 1.1.1.71 acetaldehyde + NADPH - Ralstonia sp. ethanol + NADP+ - r 439002 1.1.1.71 acetaldehyde + NADPH + H+ - Cucumis melo ethanol + NADP+ - r 139551 1.1.1.71 acetone + NADH + H+ - Ralstonia sp. propan-2-ol + NAD+ - r 396195 1.1.1.71 acetophenone + NADH + H+ - Ralstonia sp. 1-phenylethan-1-ol + NAD+ - r 439014 1.1.1.71 all-trans-retinol + NADP+ - Homo sapiens all-trans-retinal + NADPH + H+ - r 370852 1.1.1.71 allyl alcohol + NADP+ - Leuconostoc mesenteroides acrylaldehyde + NADPH - r 188561 1.1.1.71 allyl alcohol + NADP+ 28% of activity with hexan-1-ol Acinetobacter calcoaceticus acrylaldehyde + NADPH - r 188561 1.1.1.71 allyl alcohol + NADP+ - Leuconostoc mesenteroides IFO 3426 acrylaldehyde + NADPH - r 188561 1.1.1.71 benzaldehyde + NADPH - Ralstonia sp. benzyl alcohol + NADP+ - r 187480 1.1.1.71 benzaldehyde + NADPH - Ralstonia sp. DSM 6428 benzyl alcohol + NADP+ - r 187480 1.1.1.71 benzyl alcohol + NADP+ - Acinetobacter calcoaceticus benzaldehyde + NADPH - r 187589 1.1.1.71 benzyl alcohol + NADP+ - Ralstonia sp. benzaldehyde + NADPH - r 187589 1.1.1.71 benzyl alcohol + NADP+ - Ralstonia sp. DSM 6428 benzaldehyde + NADPH - r 187589 1.1.1.71 benzylalcohol + NAD+ - Pyrococcus horikoshii benzaldehyde + NADH + H+ - r 384275 1.1.1.71 butan-1,4-diol + NADP+ 29% of activity with hexan-1-ol Acinetobacter calcoaceticus butandialdehyde + NADPH - r 188563 1.1.1.71 butan-1-ol + NADP+ - Leuconostoc mesenteroides butanal + NADPH - r 188562 1.1.1.71 butan-1-ol + NADP+ 36.3% of activity with hexan-1-ol Acinetobacter calcoaceticus butanal + NADPH - r 188562 1.1.1.71 butan-1-ol + NADP+ - Leuconostoc mesenteroides IFO 3426 butanal + NADPH - r 188562 1.1.1.71 butan-1-ol + NADP+ - Saccharomyces cerevisiae butanal + NADPH + H+ - r 435361 1.1.1.71 butan-1-ol + NADP+ - Saccharomyces cerevisiae D273-10B butanal + NADPH + H+ - r 435361 1.1.1.71 butanal + NADPH + H+ - Saccharomyces cerevisiae butan-1-ol + NADP+ - r 435366 1.1.1.71 butanal + NADPH + H+ - Clostridium beijerinckii butan-1-ol + NADP+ - r 435366 1.1.1.71 butanal + NADPH + H+ - Saccharomyces cerevisiae D273-10B butan-1-ol + NADP+ - r 435366 1.1.1.71 cis-hex-3-en-1-ol + NADP+ 18.7% of activity with hexan-1ol Acinetobacter calcoaceticus cis-hex-3-en-1-al + NADPH - r 188567 1.1.1.71 cyclohexanol + NAD+ - Pyrococcus horikoshii cyclohexanone + NADH + H+ - r 384491 1.1.1.71 cyclohexanol + NADP+ - Ralstonia sp. cyclohexanone + NADH + H+ - r 439367 1.1.1.71 cyclohexanone + NADH + H+ - Ralstonia sp. cyclohexanol + NAD+ - r 372796 1.1.1.71 cyclohexanone + NADH + H+ - Ralstonia sp. cyclohexanol + NADP+ - r 439369 1.1.1.71 decan-1-ol + NADP+ decrease in enzyme activity with increase in chain length Acinetobacter calcoaceticus decanal + NADPH - r 188569 1.1.1.71 dodecan-1-ol + NADP+ decrease in enzyme activity with increase in chain length Acinetobacter calcoaceticus dodecanal + NADPH - r 188570 1.1.1.71 ethanediol + NADP+ 8.5% of activity with hexan-1-ol Acinetobacter calcoaceticus glyoxal + NADPH - r 188559 1.1.1.71 ethanol + NAD+ - Pyrococcus horikoshii acetaldehyde + NADH + H+ - r 384669 1.1.1.71 ethanol + NADP+ - Saccharomyces cerevisiae acetaldehyde + NADPH + H+ - r 187591 1.1.1.71 ethanol + NADP+ - Saccharomyces cerevisiae D273-10B acetaldehyde + NADPH + H+ - r 187591 1.1.1.71 ethanol + NADP+ - Leuconostoc mesenteroides ethanal + NADPH + H+ - r 188558 1.1.1.71 ethanol + NADP+ oxidation rate at 5% of reduction rate Moniezia expansa ethanal + NADPH + H+ - r 188558 1.1.1.71 ethanol + NADP+ 8.5% of activity with hexan-1-ol Acinetobacter calcoaceticus ethanal + NADPH + H+ - r 188558 1.1.1.71 ethanol + NADP+ - Leuconostoc mesenteroides IFO 3426 ethanal + NADPH + H+ - r 188558 1.1.1.71 ethyl acetoacetate + NADPH + H+ - Starmerella magnoliae ? + NADP+ - r 459651 1.1.1.71 heptan-1-ol + NADP+ - Acinetobacter calcoaceticus heptanal + NADPH - r 188572 1.1.1.71 hexan-1-ol + NADP+ - Saccharomyces cerevisiae hexanal + NADPH - r 188556 1.1.1.71 hexan-1-ol + NADP+ - Acinetobacter calcoaceticus hexanal + NADPH - r 188556 1.1.1.71 hexan-1-ol + NADP+ - Saccharomyces cerevisiae D273-10B hexanal + NADPH - r 188556 1.1.1.71 mannitol + NADP+ 6.5% of activity with hexan-1-ol Acinetobacter calcoaceticus D-mannose + NADPH - r 188566 1.1.1.71 methanol + NAD+ - Pyrococcus horikoshii formaldehyde + NADH + H+ - r 186834 1.1.1.71 methanol + NAD+ - Pyrococcus horikoshii DSM 12428 formaldehyde + NADH + H+ - r 186834 1.1.1.71 methanol + NAD+ - Pyrococcus horikoshii NBRC 100139 formaldehyde + NADH + H+ - r 186834 1.1.1.71 methanol + NAD+ - Pyrococcus horikoshii JCM 9974 formaldehyde + NADH + H+ - r 186834 1.1.1.71 methanol + NAD+ - Pyrococcus horikoshii ATCC 700860 formaldehyde + NADH + H+ - r 186834 1.1.1.71 methanol + NAD+ - Pyrococcus horikoshii OT-3 formaldehyde + NADH + H+ - r 186834 1.1.1.71 octan-1-ol + NADP+ decrease in enzyme activity with increase in chain length Acinetobacter calcoaceticus octanal + NADPH - r 188568 1.1.1.71 octan-1-ol + NADP+ - Saccharomyces cerevisiae octanal + NADPH + H+ - r 435672 1.1.1.71 pentan-1-ol + NADP+ - Leuconostoc mesenteroides pentanal + NADPH - r 188564 1.1.1.71 pentan-1-ol + NADP+ 4% of activity with hexan-1-ol Acinetobacter calcoaceticus pentanal + NADPH - r 188564 1.1.1.71 pentan-1-ol + NADP+ - Leuconostoc mesenteroides IFO 3426 pentanal + NADPH - r 188564 1.1.1.71 propan-1-ol + NADP+ oxidation rate at 5% of reduction rate Moniezia expansa propanal + NADPH - r 188560 1.1.1.71 propan-1-ol + NADP+ 28% of activity with hexan-1-ol Acinetobacter calcoaceticus propanal + NADPH - r 188560 1.1.1.71 retinaldehyde + NADH - Rattus norvegicus retinol + NAD+ - r 383355 1.1.1.71 retinaldehyde + NADH the enzyme is involved in retinol and vitamin A metabolism, retinoic acid is important in intestinal epithelial cell proliferation and differentiation, the enzyme also plays a role in dietary conversion of beta-carotene to retinol via retinaldehyde Rattus norvegicus retinol + NAD+ - r 383355 1.1.1.71 ribitol + NADP+ 4% of activity with hexan-1-ol Acinetobacter calcoaceticus D-ribose + NADPH - r 188565