1.1.1.6 (2R,3R)-2,3-butanediol + NAD+ - Ogataea angusta (3R)-acetoin + NADH more than 99% enantiomeric excess of R-product r 165097 1.1.1.6 (2R,3R)-2,3-butanediol + NAD+ - Ogataea angusta DL-1 (3R)-acetoin + NADH more than 99% enantiomeric excess of R-product r 165097 1.1.1.6 (2R,3R)-2,3-butanediol + NAD+ better substrate than glycerol Ogataea angusta ? + NADH - ? 371253 1.1.1.6 (2R,3R)-2,3-butanediol + NAD+ better substrate than glycerol Ogataea angusta DL-1 ? + NADH - ? 371253 1.1.1.6 (R)-1,2-propanediol + NAD+ better substrate than glycerol Ogataea angusta ? + NADH - ? 371385 1.1.1.6 (R)-1,2-propanediol + NAD+ better substrate than glycerol Ogataea angusta DL-1 ? + NADH - ? 371385 1.1.1.6 (R)-1,2-propanediol + NAD+ the enzyme prefers the R-enantiomer Thermotoga maritima hydroxyacetone + NADH - r 438552 1.1.1.6 (R)-1,2-propanediol + NAD+ the enzyme prefers the R-enantiomer Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 hydroxyacetone + NADH - r 438552 1.1.1.6 1,2,3-butanetriol + NAD+ - Schizosaccharomyces pombe 1,3-dihydroxybutane-2-one + NADH - ? 353232 1.1.1.6 1,2-butanediol + NAD+ - Thermotoga maritima ? + NADH - ? 257099 1.1.1.6 1,2-butanediol + NAD+ - Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ? + NADH - ? 257099 1.1.1.6 1,2-butanediol + NAD+ - Schizosaccharomyces pombe 1-hydroxybutane-2-one + NADH - r 353230 1.1.1.6 1,2-butanediol + NAD+ - Weizmannia coagulans 1-hydroxy-2-butanone + NADH + H+ - ? 458800 1.1.1.6 1,2-ethanediol + NAD+ - Weizmannia coagulans hydroxyacetaldehyde + NADH + H+ - ? 458818 1.1.1.6 1,2-propanediol + NAD+ - Priestia megaterium hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Klebsiella aerogenes hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Schizosaccharomyces pombe hydroxyacetone + NADH - r 353229 1.1.1.6 1,2-propanediol + NAD+ - Pichia membranifaciens hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Cellulomonas sp. hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Cellulomonas sp. hydroxyacetone + NADH - r 353229 1.1.1.6 1,2-propanediol + NAD+ 105% of the activity with glycerol Cellulomonas sp. hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ relative activity is 116% compared to oxidation of glycerol Cellulomonas sp. hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ as active as glycerol Klebsiella aerogenes hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ 1,2-propanediol represses the global activator HilA that induces an invasive phenotype and repression of HilA could be weakened by glycerol dehydrogenase activity Salmonella enterica hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Cellulomonas sp. NT3060 hydroxyacetone + NADH - r 353229 1.1.1.6 1,2-propanediol + NAD+ relative activity is 116% compared to oxidation of glycerol Cellulomonas sp. NT3060 hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Cellulomonas sp. NT3060 hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Pichia membranifaciens H 112 hydroxyacetone + NADH - ? 353229 1.1.1.6 1,2-propanediol + NAD+ - Klebsiella aerogenes P14 hydroxyacetone + NADH - ? 353229 1.1.1.6 1,3-butanediol + NAD+ relative activity is 6.5% compared to oxidation of glycerol Cellulomonas sp. ? - ? 353239 1.1.1.6 1,3-butanediol + NAD+ - Escherichia coli 4-hydroxy-2-butanone + NADH + H+ - ? 413786 1.1.1.6 1,3-butanediol + NAD+ - Klebsiella pneumoniae 4-hydroxy-2-butanone + NADH + H+ - ? 413786 1.1.1.6 1,3-butanediol + NAD+ - Salmonella enterica 4-hydroxy-2-butanone + NADH + H+ - ? 413786 1.1.1.6 1,3-butanediol + NAD+ - Weizmannia coagulans 4-hydroxy-2-butanone + NADH + H+ - ? 413786 1.1.1.6 1,3-butanediol + NAD+ - Salmonella enterica ATCC 9150 4-hydroxy-2-butanone + NADH + H+ - ? 413786 1.1.1.6 1,3-butanediol + NAD+ - Klebsiella pneumoniae MGH78578 4-hydroxy-2-butanone + NADH + H+ - ? 413786 1.1.1.6 1,3-dichloro-2-propanol + NAD+ - Klebsiella aerogenes 1,3-dichloro-2-propanone + NADH - ? 353244 1.1.1.6 1,3-dichloro-2-propanol + NAD+ - Klebsiella aerogenes P14 1,3-dichloro-2-propanone + NADH - ? 353244 1.1.1.6 1,3-propanediol + NAD+ - Clostridium butyricum ? - ? 353236 1.1.1.6 1,3-propanediol + NAD+ relative rate of oxidation is 37% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353236 1.1.1.6 1,3-propanediol + NAD+ relative rate of oxidation is 18% compared to oxidation of glycerol Cellulomonas sp. ? - ? 353236 1.1.1.6 1,3-propanediol + NAD+ - Clostridium butyricum E5 ? - ? 353236 1.1.1.6 1,4-butanediol + NAD+ relative activity 17% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353240 1.1.1.6 1,4-butanediol + NAD+ relative activity is 0.3% compared to oxidation of glycerol Cellulomonas sp. ? - ? 353240 1.1.1.6 1,4-butanediol + NAD+ - Thermus thermophilus ? + NADH + H+ - ? 440481 1.1.1.6 1,4-butanediol + NAD+ - Weizmannia coagulans ? + NADH + H+ - ? 440481 1.1.1.6 1-butanol + NAD+ - Thermus thermophilus butanal + NADH + H+ - ? 383560 1.1.1.6 1-butanol + NAD+ - Thermus thermophilus MTCC 1494 butanal + NADH + H+ - ? 383560 1.1.1.6 1-chloro-2,3-propanediol + NAD+ - Klebsiella aerogenes ? - ? 353242 1.1.1.6 1-chloro-2,3-propanediol + NAD+ - Klebsiella aerogenes P14 ? - ? 353242 1.1.1.6 1-octanol + NAD+ - Thermus thermophilus octanal + NADH + H+ - ? 383576 1.1.1.6 1-octanol + NAD+ - Thermus thermophilus MTCC 1494 octanal + NADH + H+ - ? 383576 1.1.1.6 1-phenylethan-1,2-diol + NAD+ - Arthrobacter sp. ? + NADH - ? 371525 1.1.1.6 1-phenylethanol + NAD+ - Thermus thermophilus acetophenone + NADH + H+ - ? 403287 1.1.1.6 1-propanol + NAD+ - Thermus thermophilus propanal + NADH + H+ - ? 383587 1.1.1.6 1-propanol + NAD+ - Thermus thermophilus MTCC 1494 propanal + NADH + H+ - ? 383587 1.1.1.6 2,3-butanediol + NAD+ - Schizosaccharomyces pombe 3-hydroxybutane-2-one + NADH - r 353231 1.1.1.6 2,3-butanediol + NAD+ - Clostridium butyricum 3-hydroxybutane-2-one + NADH - ? 353231 1.1.1.6 2,3-butanediol + NAD+ - Cellulomonas sp. 3-hydroxybutane-2-one + NADH - ? 353231 1.1.1.6 2,3-butanediol + NAD+ - Cellulomonas sp. 3-hydroxybutane-2-one + NADH - r 353231 1.1.1.6 2,3-butanediol + NAD+ as active as glycerol Klebsiella aerogenes 3-hydroxybutane-2-one + NADH - ? 353231 1.1.1.6 2,3-butanediol + NAD+ - Cellulomonas sp. NT3060 3-hydroxybutane-2-one + NADH - r 353231 1.1.1.6 2,3-butanediol + NAD+ - Cellulomonas sp. NT3060 3-hydroxybutane-2-one + NADH - ? 353231 1.1.1.6 2,3-butanediol + NAD+ - Clostridium butyricum E5 3-hydroxybutane-2-one + NADH - ? 353231 1.1.1.6 2-butanol + NAD+ - Thermus thermophilus 2-butanone + NADH + H+ - ? 383745 1.1.1.6 2-propanol + NAD+ - Thermus thermophilus acetone + NADH + H+ - ? 371770 1.1.1.6 2-propanol + NAD+ - Thermus thermophilus MTCC 1494 acetone + NADH + H+ - ? 371770 1.1.1.6 3-(2,2,2-trifluoroethoxy)propane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-(2,2,2-trifluoroethoxy)propan-2-one + NADH + H+ - ? 459013 1.1.1.6 3-(2,2,2-trifluoroethoxy)propane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460580 1.1.1.6 3-amino-1,2-propanediol + NAD+ 104% of the activity with glycerol Cellulomonas sp. ? - ? 353261 1.1.1.6 3-bromo-1,2-propanediol + NAD+ 109% of the activity with glycerol Cellulomonas sp. ? - ? 353260 1.1.1.6 3-butoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-butoxypropan-2-one + NADH + H+ - ? 459022 1.1.1.6 3-butoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460587 1.1.1.6 3-chloro-1,2-propanediol + NAD+ 130% of the activity with glycerol Cellulomonas sp. ? - ? 353259 1.1.1.6 3-ethoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-ethoxypropan-2-one + NADH + H+ - ? 459024 1.1.1.6 3-ethoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460589 1.1.1.6 3-hydroxypropionaldehyde + NADH - Clostridium butyricum propan-1,3-diol + NAD+ - ? 353253 1.1.1.6 3-mercapto-1,2-propanediol + NAD+ 155% of the activity with glycerol Cellulomonas sp. ? - ? 353258 1.1.1.6 3-methoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-methoxypropan-2-one + NADH + H+ - ? 459038 1.1.1.6 3-methoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460608 1.1.1.6 3-phenoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-phenoxypropan-2-one + NADH + H+ - ? 459048 1.1.1.6 3-phenoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460628 1.1.1.6 3-propoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-propoxypropan-2-one + NADH + H+ - ? 459050 1.1.1.6 3-propoxypropane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460634 1.1.1.6 3-[(propan-2-yl)oxy]propane-1,2-diol + NAD+ - Geobacillus stearothermophilus 1-hydroxy-3-[(propan-2-yl)oxy]propan-2-one + NADH + H+ - ? 459051 1.1.1.6 3-[(propan-2-yl)oxy]propane-1,2-diol + NAD+ - Geobacillus stearothermophilus ? + NADH + H+ - ? 460636 1.1.1.6 acetaldehyde + NADH + H+ - Clostridium butyricum ethanol + NAD+ - ? 139564 1.1.1.6 acetaldehyde + NADH + H+ - Clostridium butyricum E5 ethanol + NAD+ - ? 139564 1.1.1.6 acetoin + NADH + H+ - Ogataea angusta 2,3-butanediol + NAD+ - r 165093 1.1.1.6 acetoin + NADH + H+ - Ogataea angusta DL-1 2,3-butanediol + NAD+ - r 165093 1.1.1.6 benzyl alcohol + NAD+ - Thermus thermophilus benzaldehyde + NADH + H+ - ? 384271 1.1.1.6 beta-glycerophosphate + NAD+ relative activity is 2% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353249 1.1.1.6 butane-1,3-diol + NAD+ - Arthrobacter sp. ? + NADH - ? 372588 1.1.1.6 butane-2,3-diol + NAD+ - Klebsiella pneumoniae ? + NADH + H+ - ? 459501 1.1.1.6 butane-2,3-diol + NAD+ - Klebsiella pneumoniae ATCC 700721 ? + NADH + H+ - ? 459501 1.1.1.6 D-1,2-propanediol + NAD+ - Weizmannia coagulans hydroxyacetone + NADH + H+ - ? 459557 1.1.1.6 D-2,3-butanediol + NAD+ - Weizmannia coagulans ? + NADH + H+ - ? 459558 1.1.1.6 D-glyceraldehyde + NADH + H+ - Thermus thermophilus glycerol + NAD+ - r 445398 1.1.1.6 diglycerol + NAD+ relative activity is 21% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353248 1.1.1.6 dihydroxyacetone + NADH + H+ - Clostridium butyricum glycerol + NAD+ - r 405002 1.1.1.6 DL-alpha-glycerophosphate + NAD+ relative activity 11%compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353241 1.1.1.6 DL-alpha-glycerophosphate + NAD+ oxidation at 16% compared to oxidation of glycerol Cellulomonas sp. ? - ? 353241 1.1.1.6 DL-glyceraldehyde + NAD+ - Schizosaccharomyces pombe 3-hydroxypyruvaldehyde + NADH - ? 353233 1.1.1.6 DL-glyceraldehyde + NAD+ - Pichia membranifaciens 3-hydroxypyruvaldehyde + NADH - ? 353233 1.1.1.6 DL-glyceraldehyde + NAD+ - Cellulomonas sp. 3-hydroxypyruvaldehyde + NADH - ? 353233 1.1.1.6 DL-glyceraldehyde + NAD+ relative rate of reduction is 14%compared to reduction of dihydroxyacetone Klebsiella aerogenes 3-hydroxypyruvaldehyde + NADH - ? 353233 1.1.1.6 DL-glyceraldehyde + NAD+ - Pichia membranifaciens H 112 3-hydroxypyruvaldehyde + NADH - ? 353233 1.1.1.6 DL-glyceraldehyde + NADH - Clostridium butyricum glycerol + NAD+ - ? 187043 1.1.1.6 DL-glyceraldehyde + NADH - Clostridium butyricum E5 glycerol + NAD+ - ? 187043 1.1.1.6 erythrite + NAD+ - Priestia megaterium ? - ? 353235 1.1.1.6 ethanediol + NAD+ - Schizosaccharomyces pombe glycolaldehyde + NADH - ? 353228 1.1.1.6 ethanediol + NAD+ - Cellulomonas sp. glycolaldehyde + NADH reduction at concentration of 50 mM r 353228 1.1.1.6 ethanediol + NAD+ - Cellulomonas sp. NT3060 glycolaldehyde + NADH reduction at concentration of 50 mM r 353228 1.1.1.6 ethanol + NAD+ relative activity is 1% compared to oxidation of glycerol Klebsiella aerogenes acetaldehyde + NADH - ? 186836 1.1.1.6 ethanol + NAD+ - Thermus thermophilus acetaldehyde + NADH + H+ - ? 384669 1.1.1.6 ethylene glycol + NAD+ - Cellulomonas sp. ? - ? 353238 1.1.1.6 ethylene glycol + NAD+ - Klebsiella pneumoniae subsp. pneumoniae ? - ? 353238 1.1.1.6 ethylene glycol + NAD+ relative activity is 20% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353238 1.1.1.6 ethylene glycol + NAD+ - Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 ? - ? 353238 1.1.1.6 ethylene glycol + NAD+ - Thermus thermophilus ? + NADH + H+ - ? 445479 1.1.1.6 ethylene glycol + NAD+ - Klebsiella pneumoniae ? + NADH + H+ - ? 445479 1.1.1.6 ethylene glycol + NAD+ - Klebsiella pneumoniae ATCC 700721 ? + NADH + H+ - ? 445479 1.1.1.6 glycerol + N6-carboxymethyl-NAD+ - Thermotoga maritima glycerone + N6-carboxymethyl-NADH + H+ - r 451088 1.1.1.6 glycerol + N6-carboxymethyl-NAD+ - Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 glycerone + N6-carboxymethyl-NADH + H+ - r 451088 1.1.1.6 glycerol + N6-CM-NAD+ - Thermotoga maritima glycerone + N6-carboxymethyl-NADH + H+ - r 451089 1.1.1.6 glycerol + N6-CM-NAD+ - Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 glycerone + N6-carboxymethyl-NADH + H+ - r 451089 1.1.1.6 glycerol + NAD+ - Escherichia coli dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Priestia megaterium dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Klebsiella aerogenes dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Schizosaccharomyces pombe dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Schizosaccharomyces pombe dihydroxyacetone + NADH + H+ - r 353227 1.1.1.6 glycerol + NAD+ - Pichia membranifaciens dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Escherichia coli K-12 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Cellulomonas sp. dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Cellulomonas sp. dihydroxyacetone + NADH + H+ - r 353227 1.1.1.6 glycerol + NAD+ Glycerol dehydrogenase was immobilised in a polycarbamoyl sulfonate-hydrogel to be used as a sensor for glycerol. Glycerol oxidation leads to the reduction of NAD+ to NADH and electrons are transferred to ferricyanide on an electrode surface Cellulomonas sp. dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate Escherichia coli dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ the enzyme exhibits an exclusive preference for NAD+ over NADP+ Klebsiella pneumoniae subsp. pneumoniae dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ the enzyme exhibits an exclusive preference for NAD+ over NADP+ Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae KG1 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Cellulomonas sp. NT3060 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Cellulomonas sp. NT3060 dihydroxyacetone + NADH + H+ - r 353227 1.1.1.6 glycerol + NAD+ - Pichia membranifaciens H 112 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Schizosaccharomyces pombe 972h- dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate Escherichia coli JM109 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Escherichia coli JM109 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae ATCC 700721 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Klebsiella aerogenes P14 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Klebsiella aerogenes 1033 dihydroxyacetone + NADH + H+ - ? 353227 1.1.1.6 glycerol + NAD+ - Escherichia coli glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Escherichia coli glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Serratia marcescens glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Thermoanaerobacterium thermosaccharolyticum glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Ogataea angusta glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Arthrobacter sp. glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Serratia sp. glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Cellulomonas sp. glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Thermotoga maritima glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae subsp. pneumoniae glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Geobacillus stearothermophilus glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Weizmannia coagulans glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ no activity detected with NADP+ Schizosaccharomyces pombe glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ glycerone reduction is the dominant reaction Thermoanaerobacterium thermosaccharolyticum glycerone + NADH + H+ i.e. 1,3-dihydroxypropranone r 373292 1.1.1.6 glycerol + NAD+ activity of the enzyme is confirmed by proteome analysis and enzyme assays with cell extract glycerol-grown cells Anaerobium acetethylicum glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Klebsiella pneumoniae DSM 2026 glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Thermoanaerobacterium thermosaccharolyticum DSM 571 glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ glycerone reduction is the dominant reaction Thermoanaerobacterium thermosaccharolyticum DSM 571 glycerone + NADH + H+ i.e. 1,3-dihydroxypropranone r 373292 1.1.1.6 glycerol + NAD+ - Escherichia coli JM109 glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Escherichia coli K12 glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Ogataea angusta DL-1 glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ - Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 glycerone + NADH + H+ - r 373292 1.1.1.6 glycerol + NAD+ no activity detected with NADP+ Schizosaccharomyces pombe ARC039 glycerone + NADH + H+ - ? 373292 1.1.1.6 glycerol + NAD+ - Escherichia coli D-glyceraldehyde + NADH + H+ - r 383174 1.1.1.6 glycerol + NAD+ - Geobacillus stearothermophilus D-glyceraldehyde + NADH + H+ - ? 383174 1.1.1.6 glycerol + NAD+ no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde Thermus thermophilus D-glyceraldehyde + NADH + H+ - r 383174 1.1.1.6 glycerol + NAD+ - Escherichia coli JM109 D-glyceraldehyde + NADH + H+ - r 383174 1.1.1.6 glycerol + NAD+ no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde Thermus thermophilus MTCC 1494 D-glyceraldehyde + NADH + H+ - r 383174 1.1.1.6 glycerol + NADP+ the enzyme exhibits an exclusive preference for NAD+ over NADP+ Klebsiella pneumoniae subsp. pneumoniae dihydroxyacetone + NADPH + H+ - ? 187357 1.1.1.6 glycerol + NADP+ the enzyme exhibits an exclusive preference for NAD+ over NADP+ Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 dihydroxyacetone + NADPH + H+ - ? 187357 1.1.1.6 glycerol + NADP+ - Mucor circinelloides glyceraldehyde + NADPH + H+ - ? 414663 1.1.1.6 glycerol + NADP+ activity observed with pentan-1-ol, 3-methyl-butan-1-ol, 1-decanol, low activity as ethanol dehydrogenase with NAD+ or NADPH+ as cofactor Mucor circinelloides glyceraldehyde + NADPH + H+ - ? 414663 1.1.1.6 glycerol + NADP+ - Mucor circinelloides YR-1 glyceraldehyde + NADPH + H+ - ? 414663 1.1.1.6 glycerol + NADP+ activity observed with pentan-1-ol, 3-methyl-butan-1-ol, 1-decanol, low activity as ethanol dehydrogenase with NAD+ or NADPH+ as cofactor Mucor circinelloides YR-1 glyceraldehyde + NADPH + H+ - ? 414663 1.1.1.6 glycerol-alpha-monochlorohydrin + NAD+ relative rate of oxidation is 26% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353237 1.1.1.6 glycerol-alpha-monochlorohydrin + NAD+ relative rate of oxidation is 71% compared to oxidation of glycerol Cellulomonas sp. ? - ? 353237 1.1.1.6 glycerol-alpha-monomethyl ether + NAD+ - Klebsiella aerogenes ? - ? 353243 1.1.1.6 glycerol-alpha-monomethyl ether + NAD+ - Klebsiella aerogenes P14 ? - ? 353243 1.1.1.6 glycerone + NADH + H+ - Ogataea angusta glycerol + NAD+ - r 373319 1.1.1.6 glycerone + NADH + H+ - Ogataea angusta DL-1 glycerol + NAD+ - r 373319 1.1.1.6 hydroxy-2-propanone + NADH relative activity is 27% compared to oxidation of glycerol Klebsiella aerogenes propylene glycol + NAD+ - ? 353251 1.1.1.6 i-inositol + NAD+ relative activity is 18% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353246 1.1.1.6 isopropanol + NAD+ relative activity is 17% compared to oxidation of glycerol Klebsiella aerogenes acetone + NADH - ? 353245 1.1.1.6 L-1,2-propanediol + NAD+ - Weizmannia coagulans hydroxyacetone + NADH + H+ - ? 459763 1.1.1.6 L-2,3-butanediol + NAD+ - Weizmannia coagulans ? + NADH + H+ - ? 459764 1.1.1.6 meso-2,3-butanediol + NAD+ - Ogataea angusta (3S)-acetoin + NADH more than 99% enantiomeric excess of S-product ? 373805 1.1.1.6 meso-2,3-butanediol + NAD+ - Ogataea angusta DL-1 (3S)-acetoin + NADH more than 99% enantiomeric excess of S-product ? 373805 1.1.1.6 methylglyoxal + NADH - Schizosaccharomyces pombe lactaldehyde + NAD+ - ? 353234 1.1.1.6 methylglyoxal + NADH relative rate of reduction is 56% compared to reduction of dihydroxyacetone Klebsiella aerogenes lactaldehyde + NAD+ - ? 353234 1.1.1.6 additional information high specificity of enzyme for secondary alcohols in R-configuration Ogataea angusta ? - ? 89 1.1.1.6 additional information no substrate: 1,3-propanediol, ethanol, 1-propanol, 2-propanol, propionic acid, 1,4-butanediol, sorbitol, L-iditol. Stereospecificity for R-form Ogataea angusta ? - ? 89 1.1.1.6 additional information ability of the enzyme to use glycerol from biodiesel waste as substrate, overview Escherichia coli ? - ? 89 1.1.1.6 additional information efficiency of a cofactor regeneration enzyme co-expressed with a glycerol dehydrogenase for the production of 1,3-dihydroxyacetone. In vitro biotransformation of glycerol is achieved with the cell-free extracts containing recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, or NADH oxidase LpNox1 from Lactobacillus pentosus, giving1,3-dihydroxyacetone, overview Escherichia coli ? - ? 89 1.1.1.6 additional information enzyme TtGlyDH preferentially catalyzes 1,3-dihydroxypropranone reduction rather than alcohol compound oxidation. Glycerol oxidization activity is faintly detected in the presence of a high concentration of glycerol (137 mM). No activity is detected with primary alcohols or diols. The highest glycerol oxidation activity is observed at the optimal growth temperature of 60°C in Tris-HCl buffer (50 mM, pH 8.0). Maximum DHA reduction activity is also observed at 60°C, and TtGlyDH exhibits the highest activity in an acetate buffer, compared with 91% maximum activity in an imidazole buffer at the same pH of 6.0 Thermoanaerobacterium thermosaccharolyticum ? - ? 89 1.1.1.6 additional information the enzyme is also active with substrates 4-chloroacetoacetate, 3-chloroacetylpyridine, 4-chloroacetophenone, and acetophenone, substrate specificities of enzyme with bound Zn2+, Mn2+, or Mg2+, overview Klebsiella pneumoniae subsp. pneumoniae ? - ? 89 1.1.1.6 additional information enzyme is highly enantioselective towards S-isomers of substituted glycerol derivatives Geobacillus stearothermophilus ? - - 89 1.1.1.6 additional information the enzyme is also active with substrates 4-chloroacetoacetate, 3-chloroacetylpyridine, 4-chloroacetophenone, and acetophenone, substrate specificities of enzyme with bound Zn2+, Mn2+, or Mg2+, overview Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 ? - ? 89 1.1.1.6 additional information enzyme TtGlyDH preferentially catalyzes 1,3-dihydroxypropranone reduction rather than alcohol compound oxidation. Glycerol oxidization activity is faintly detected in the presence of a high concentration of glycerol (137 mM). No activity is detected with primary alcohols or diols. The highest glycerol oxidation activity is observed at the optimal growth temperature of 60°C in Tris-HCl buffer (50 mM, pH 8.0). Maximum DHA reduction activity is also observed at 60°C, and TtGlyDH exhibits the highest activity in an acetate buffer, compared with 91% maximum activity in an imidazole buffer at the same pH of 6.0 Thermoanaerobacterium thermosaccharolyticum DSM 571 ? - ? 89 1.1.1.6 additional information high specificity of enzyme for secondary alcohols in R-configuration Ogataea angusta DL-1 ? - ? 89 1.1.1.6 additional information no substrate: 1,3-propanediol, ethanol, 1-propanol, 2-propanol, propionic acid, 1,4-butanediol, sorbitol, L-iditol. Stereospecificity for R-form Ogataea angusta DL-1 ? - ? 89 1.1.1.6 N-butyraldehyde + NADH - Clostridium butyricum 1-butanol + NAD+ - ? 353257 1.1.1.6 propane-1,2-diol + NAD+ - Arthrobacter sp. propane-1-ol-2-one + NADH - ? 374425 1.1.1.6 propionaldehyde + NADH - Clostridium butyricum 1-propanol + NAD+ - ? 353256 1.1.1.6 pyruvate + NADH + H+ - Weizmannia coagulans D-lactate + NADH - ? 459881 1.1.1.6 R-1-amino-2-propanol + NAD+ 33% of the activity with glycerol Cellulomonas sp. ? - ? 353262 1.1.1.6 racemic 2,3-butanediol + NAD+ - Klebsiella pneumoniae subsp. pneumoniae 3-hydroxybutane-2-one + NADH + H+ - ? 459886 1.1.1.6 racemic 2,3-butanediol + NAD+ - Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 3-hydroxybutane-2-one + NADH + H+ - ? 459886 1.1.1.6 S-1-amino-2-propanol + NAD+ 9% of the activity with glycerol Cellulomonas sp. ? - ? 353263 1.1.1.6 sorbitol + NAD+ relative activity is 3% compared to oxidation of glycerol Klebsiella aerogenes ? - ? 353247