1.1.1.53 11-deoxycortisol + NADH + H+ i.e. 4-pregnen-17,21-diol-3,20-dione, 49.8% of the activity compared to cortisone Agathobaculum desmolans ? + NAD+ - ? 460424 1.1.1.53 11-deoxycortisol + NADH + H+ i.e. 4-pregnen-17,21-diol-3,20-dione, 49.8% of the activity compared to cortisone Agathobaculum desmolans ATCC 43058 ? + NAD+ - ? 460424 1.1.1.53 11alpha-hemisuccinyloxyprogesterone + NADH - Streptomyces exfoliatus ? - ? 188336 1.1.1.53 17-hydroxy-3,11,20-trioxo-4-pregnen-21-oate + NADH - Streptomyces exfoliatus 17,20-dihydroxy-3,11-dioxo-4-pregnen-21-oate + NAD+ - ? 188339 1.1.1.53 17-hydroxy-3,20-dioxo-4-pregnen-21-oate + NADH - Streptomyces exfoliatus 17,20-dihydroxy-4-pregnen-3-one-21-oate + NAD+ - ? 188337 1.1.1.53 17-hydroxyprogesterone + NADH - Streptomyces exfoliatus ? - ? 188324 1.1.1.53 17-hydroxyprogesterone + NADPH - Oncorhynchus mykiss 17,20-beta-dihydroxy-4-pregnene-3-one - ? 188325 1.1.1.53 17alpha,20beta-dihydroxypregn-4-ene-3-one + NADP+ - Sus scrofa 17alpha-hydroxypregn-4-en-3,20-dione + NADPH - ? 188344 1.1.1.53 17alpha-hydroxy-3,20-dioxo-4-pregnen-21-oate + NADH - Gallus gallus 17,20alpha-dihydroxy-4-pregnen-3-one-21-oate + NAD+ - ? 383606 1.1.1.53 17alpha-hydroxy-3,20-dioxo-4-pregnen-21-oate + NADH - Oncorhynchus mykiss 17,20alpha-dihydroxy-4-pregnen-3-one-21-oate + NAD+ - ? 383606 1.1.1.53 17alpha-hydroxy-3,20-dioxo-4-pregnen-21-oate + NADH - Channa striata 17,20alpha-dihydroxy-4-pregnen-3-one-21-oate + NAD+ - ? 383606 1.1.1.53 17alpha-hydroxypregnenolone + NADPH i.e. 3beta,17alpha-dihydroxypregn-5-ene-20-one Sus scrofa 3beta,17alpha,20beta-trihydroxypregn-5-ene + NADP+ - ? 188328 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Rattus norvegicus 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Rattus norvegicus 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - r 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Sus scrofa 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Oncorhynchus mykiss 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Streptomyces exfoliatus 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Oncorhynchus masou rhodurus 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Anguilla japonica 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ 17alpha-hydroxyprogesterone reduction in non-flagellated germ cells and in spermatozoa Oncorhynchus mykiss 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ enzyme wild type A and B, mutants of enzyme type A and B Oncorhynchus mykiss 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ - ? 188323 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Rattus norvegicus 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ - ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Sus scrofa 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ - ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Clarias gariepinus 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ - ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Oncorhynchus mykiss 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Oncorhynchus masou rhodurus 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ - Anguilla japonica 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ 20beta-hydroxysteroid dehydrogenase activity is reduced in diabetic rats Rattus norvegicus 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ - r 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ 4.3% of the activity with p-nitrobenzaldehyde Danio rerio 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ - ? 369977 1.1.1.53 17alpha-hydroxyprogesterone + NADPH + H+ 4.3% of the activity with p-nitrobenzaldehyde Oreochromis niloticus 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ - ? 369977 1.1.1.53 17beta-estradiol + NAD+ 4% of the activity with androsterone Mycobacterium tuberculosis estrone + NADH + H+ - ? 188057 1.1.1.53 17beta-estradiol + NAD+ 4% of the activity with androsterone Mycobacterium tuberculosis H37Rv estrone + NADH + H+ - ? 188057 1.1.1.53 17beta-hydroxyandrostan-2-one + NADH + H+ - Streptomyces exfoliatus ? - r 451070 1.1.1.53 17beta-[(1S)-1-hydroxy-2-propynyl]-androst-4-ene-3-one + NAD+ - Streptomyces exfoliatus 17beta-[1-oxo-2-propynyl]-androst-4-ene-3-one + NADH - ? 188331 1.1.1.53 20alpha-hydroxyprogesterone + NAD+ 2% of the activity with androsterone Mycobacterium tuberculosis ? + NADH - ? 371777 1.1.1.53 20alpha-hydroxyprogesterone + NAD+ 2% of the activity with androsterone Mycobacterium tuberculosis H37Rv ? + NADH - ? 371777 1.1.1.53 20beta-hydroxycortisone + NADH + H+ under in vitro conditions, the enzyme catalyzes reductive reaction far more readily than oxidative reaction Agathobaculum desmolans cortisone + NADH + H+ - r 460560 1.1.1.53 20beta-hydroxycortisone + NADH + H+ under in vitro conditions, the enzyme catalyzes reductive reaction far more readily than oxidative reaction Agathobaculum desmolans ATCC 43058 cortisone + NADH + H+ - r 460560 1.1.1.53 20beta-hydroxypregn-4-ene-3-one + NADP+ - Sus scrofa pregn-4-ene-3,20-dione + NADPH - ? 188345 1.1.1.53 20beta-hydroxyprogesterone + NAD+ 69% of the activity with androsterone Mycobacterium tuberculosis ? + NADH - ? 371778 1.1.1.53 20beta-hydroxyprogesterone + NAD+ 69% of the activity with androsterone Mycobacterium tuberculosis H37Rv ? + NADH - ? 371778 1.1.1.53 21-acetoxyprogesterone + NADH - Streptomyces exfoliatus ? - ? 188335 1.1.1.53 21-hemisuccinyloxyprogesterone + NADH - Streptomyces exfoliatus ? - ? 188334 1.1.1.53 3,20-dioxo-4-pregnen-21-oic acid + NADH - Streptomyces exfoliatus 20beta-hydroxy-3-oxo-4-pregnen-21-oic acid + NAD+ - ? 188338 1.1.1.53 3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H enzyme belongs to the group of short-chain dehydrogenases Streptomyces exfoliatus ? - ? 369975 1.1.1.53 3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H enzyme belongs to the group of short-chain dehydrogenases Streptomyces exfoliatus ? - r 369975 1.1.1.53 3beta,17alpha,20alpha-trihydroxypregn-5-ene + NADP+ - Sus scrofa 3beta,17alpha-dihydroxypregn-5-en-20-one + NADPH - ? 188347 1.1.1.53 3beta,20alpha-dihydroxypregn-5-ene + NADP+ - Sus scrofa 3beta-hydroxypregn-5-en-20-one + NADPH - ? 188346 1.1.1.53 4-acetyl-N-(cyclohexylcarbamoyl)benzenesulfonamide + NADPH male-specific and androgen-dependent activity, competitive to progesterone, cortisone, cortisol and 17alpha-hydroxyprogestrone consumption Rattus norvegicus hydroxyhexamide + NADP+ - ? 188348 1.1.1.53 4-nitrobenzaldehyde + NADPH - Sus scrofa ? - ? 188353 1.1.1.53 4-pregnen-11beta,21-diol-3,20-dione + NADPH - Gallus gallus 4-pregnen-11beta,20alpha,21-triol-3-one + NADP+ - ? 383932 1.1.1.53 5alpha-androstan-17beta-ol-3-one + NAD(P)H + H+ i.e. 5alpha-dihydrotestosterone Sus scrofa 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ - ? 188340 1.1.1.53 5alpha-androstan-17beta-ol-3-one + NAD(P)H + H+ i.e. 5alpha-dihydrotestosterone Sus scrofa 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ ratio 4:3 ? 188340 1.1.1.53 5alpha-androstan-3alpha-ol-17-one + alpha-NAD+ - Sus scrofa 5alpha-dihydrotestosterone + alpha-NADH + H+ - r 451072 1.1.1.53 5alpha-androstan-3alpha-ol-17-one + beta-NAD+ - Sus scrofa 5alpha-dihydrotestosterone + beta-NADH + H+ - r 451075 1.1.1.53 5alpha-androstane-3,17-dione + NADPH - Sus scrofa ? - ? 188343 1.1.1.53 5alpha-dihydrotestosterone + alpha-NADH + H+ - Sus scrofa 5alpha-androstan-3alpha-ol-17-one + alpha-NAD+ - r 451073 1.1.1.53 5alpha-dihydrotestosterone + alpha-NADPH + H+ - Sus scrofa 5alpha-androstan-3alpha-ol-17-one + alpha-NADP+ - r 451074 1.1.1.53 5alpha-dihydrotestosterone + beta-NADH + H+ - Sus scrofa 5alpha-androstan-3alpha-ol-17-one + beta-NAD+ - r 451076 1.1.1.53 5alpha-dihydrotestosterone + NADP+ - Homo sapiens 5alpha-androstane-3,17-dione + NADPH + H+ - ? 188305 1.1.1.53 5alpha-dihydrotestosterone + NADPH - Rattus norvegicus 5alpha-androstan-3alpha,17beta-diol + NAD+ - ? 459105 1.1.1.53 5alpha-dihydrotestosterone + NADPH + H+ 14.4% of the activity with p-nitrobenzaldehyde Oreochromis niloticus 5alpha-androstan-3alpha,17beta-diol + NADP+ - ? 231995 1.1.1.53 5alpha-pregnane-3,20-dione + NADH - Streptomyces exfoliatus ? - ? 188332 1.1.1.53 5alpha-pregnane-3,20-dione + NADPH - Sus scrofa ? - ? 188342 1.1.1.53 5beta-androstan-17beta-ol-3-one + NAD(P)H i.e. 5beta-dihydrotestosterone Sus scrofa ? - ? 188341 1.1.1.53 5beta-dihydrotestosterone + NADPH 9.2% of the activity with p-nitrobenzaldehyde Oreochromis niloticus 5beta-androstan-3alpha,17beta-diol + NADP+ - ? 372164 1.1.1.53 6beta-(bromoacetoxy)-progesterone + NADH - Streptomyces exfoliatus ? - ? 188333 1.1.1.53 6beta-[bromoacetoxy]-progesterone + NAD+ - Streptomyces exfoliatus ? - r 451071 1.1.1.53 9,10-phenanthrenequinone + NADPH - Sus scrofa ? - ? 188352 1.1.1.53 androsterone + NAD+ - Mycobacterium tuberculosis ? + NADH - ? 372394 1.1.1.53 androsterone + NAD+ - Mycobacterium tuberculosis H37Rv ? + NADH - ? 372394 1.1.1.53 camphorquinone + NADPH + H+ - Sus scrofa camphorquinol + NADP+ - ? 188355 1.1.1.53 corticosterone + NADH + H+ i.e. 4-pregnen-11beta,21-diol-3,20-dione, 39.8% of the activity compared to cortisone Agathobaculum desmolans ? + NAD+ - ? 460886 1.1.1.53 corticosterone + NADH + H+ i.e. 4-pregnen-11beta,21-diol-3,20-dione, 39.8% of the activity compared to cortisone Agathobaculum desmolans ATCC 43058 ? + NAD+ - ? 460886 1.1.1.53 cortisone + NAD(P)H - Rattus norvegicus 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+ - ? 188330 1.1.1.53 cortisone + NAD(P)H - Streptomyces exfoliatus 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+ - ? 188330 1.1.1.53 cortisone + NAD(P)H i.e. 17beta,21-dihydroxy-pregn-4-ene-3,11,20-trione Streptomyces exfoliatus 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+ - ? 188330 1.1.1.53 cortisone + NADH + H+ the equilibrium for the recombinant enzyme clearly favors the reductive direction. Cortisol is the preferred substrate, as it is more rapidly converted to 20beta-dihydrocortisol relative to other structurally similar steroids tested Agathobaculum desmolans 20beta-hydroxycortisone + NAD+ - r 460890 1.1.1.53 cortisone + NADH + H+ the equilibrium for the recombinant enzyme clearly favors the reductive direction. Cortisol is the preferred substrate, as it is more rapidly converted to 20beta-dihydrocortisol relative to other structurally similar steroids tested Agathobaculum desmolans ATCC 43058 20beta-hydroxycortisone + NAD+ - r 460890 1.1.1.53 cortisone + NADPH + H+ - Danio rerio 20beta-hydroxycortisone + NADP+ i.e. 4-pregnen-17alpha,20beta,21-triol-3,11-dione ? 426141 1.1.1.53 cortisone + NADPH + H+ no activity with NAD+ Danio rerio 20beta-hydroxycortisone + NADP+ - ? 426141 1.1.1.53 cyclohexanone + NADPH - Sus scrofa cyclohexanol + NADP+ - ? 187643 1.1.1.53 deoxycorticosterone + NADPH - Streptomyces exfoliatus 20,21-dihydroxypregn-4-ene-3-one + NADP+ - ? 188327 1.1.1.53 deoxycorticosterone + NADPH i.e. 21-hydroxypregn-4-ene-3,20-dione, 21-hydroxyprogesterone Sus scrofa 20,21-dihydroxypregn-4-ene-3-one + NADP+ - ? 188327 1.1.1.53 deoxycortisol + NADPH - Sus scrofa ? - ? 188329 1.1.1.53 epiandrosterone + NAD+ 3% of the activity with androsterone Mycobacterium tuberculosis ? + NADH - ? 373151 1.1.1.53 epiandrosterone + NAD+ 3% of the activity with androsterone Mycobacterium tuberculosis H37Rv ? + NADH - ? 373151 1.1.1.53 hydrindantin + NADPH - Sus scrofa ? - ? 188349 1.1.1.53 menadione + NADPH 75% of the activity with p-nitrobenzaldehyde Oreochromis niloticus ? - ? 187323 1.1.1.53 methylglyoxal + NADPH - Sus scrofa ? - ? 187326 1.1.1.53 additional information enzyme cannot catalyze the reduction of 11-hydroxy- or 11-oxo-C21-steroids, i.e. cortisol, corticosterone, cortisone Sus scrofa ? - ? 89 1.1.1.53 additional information constitutive enzyme Danio rerio ? - ? 89 1.1.1.53 additional information the enzyme plays an important role in the meiotic maturation of tilapia gametes Oreochromis niloticus ? - ? 89 1.1.1.53 additional information catalyzes the NADPH reduction of carbonyl compounds and steroids, steroidogenic enzyme, key enzyme for the production of maturation-induced steroid Gadus morhua ? - ? 89 1.1.1.53 additional information catalyzes the oxidoreduction of a variety of steroid substrates, including the steroid antibiotic fusidic acid, catalyzes the interconversion of hydroxy and oxo groups at position 3 of the steroid ring structure, enzyme is capable of catalyzing the carbonyl reduction of non-steroidal xenobiotic carbonyl compounds, contributes to the bioremediation of natural and synthetic toxicants, plays a central role in steroid metabolism Comamonas testosteroni ? - ? 89 1.1.1.53 p-nitrobenzaldehyde + NADPH - Oreochromis niloticus p-nitrobenzyl alcohol + NADP+ - ? 187331 1.1.1.53 phenylglyoxal + NADPH - Sus scrofa ? - ? 187327 1.1.1.53 pregnenolone + NADPH i.e. 3-hydroxypregn-5-en-20-one Sus scrofa 3beta,20beta-Dihydroxypregn-5-ene + NADP+ - ? 188326 1.1.1.53 progesterone + NAD(P)H - Rattus norvegicus 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+ - ? 188322 1.1.1.53 progesterone + NAD(P)H - Rattus norvegicus 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+ - r 188322 1.1.1.53 progesterone + NAD(P)H - Sus scrofa 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+ - ? 188322 1.1.1.53 progesterone + NAD(P)H - Streptomyces exfoliatus 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+ - ? 188322 1.1.1.53 progesterone + NADH 22% of the activity with androsterone Mycobacterium tuberculosis 20beta-hydroxy-pregn-4-ene-3-one + NAD+ - ? 374421 1.1.1.53 progesterone + NADP+ - Homo sapiens 20beta-hydroxy-pregn-4-ene-3-one + NADPH + H+ - ? 437833 1.1.1.53 progesterone + NADPH - Rattus norvegicus 20beta-hydroxy-pregn-4-ene-3-one + NADP+ - ? 369976 1.1.1.53 progesterone + NADPH - Sus scrofa 20beta-hydroxy-pregn-4-ene-3-one + NADP+ - ? 369976 1.1.1.53 progesterone + NADPH 20beta-hydroxysteroid dehydrogenase activity is reduced in diabetic rats Rattus norvegicus 20beta-hydroxy-pregn-4-ene-3-one + NADP+ - r 369976 1.1.1.53 progesterone + NADPH 2.5% of the activity with p-nitrobenzaldehyde Oreochromis niloticus 20beta-hydroxy-pregn-4-ene-3-one + NADP+ - ? 369976 1.1.1.53 tetrahydrocortisol + NADH + H+ i.e. 5beta-pregnan-3alpha,11beta,17,21-tetrol-20-one, 49.2% of the activity compared to cortisone Agathobaculum desmolans ? + NAD+ - ? 461372 1.1.1.53 tetrahydrocortisol + NADH + H+ i.e. 5beta-pregnan-3alpha,11beta,17,21-tetrol-20-one, 49.2% of the activity compared to cortisone Agathobaculum desmolans ATCC 43058 ? + NAD+ - ? 461372