1.1.1.320	(S)-benzoin + NADP+	stereospecific asymmetric reduction of benzil to (S)-benzoin	Bacillus cereus	benzil + NADPH + H+	-	r	417829	
1.1.1.320	(S)-benzoin + NADP+	stereospecific asymmetric reduction of benzil to (S)-benzoin, recombinant Bacillus cereus benzil reductase produces optically pure (S)-benzoin with NADPH in vitro	Bacillus cereus	benzil + NADPH + H+	-	r	417829	
1.1.1.320	(S)-benzoin + NADP+	stereospecific asymmetric reduction of benzil to (S)-benzoin	Bacillus cereus Tim-r01	benzil + NADPH + H+	-	r	417829	
1.1.1.320	1-(2-fluorophenyl)ethanone + NADPH + H+	76% of the rate with 1-(4-nitrophenyl)ethanone, 97% yield, 94% enantiomeric excess	Ogataea glucozyma	(1S)-1-(2-fluorophenyl)ethanol + NADP+	-	?	438616	
1.1.1.320	1-(2-nitrophenyl)ethanone + NADPH + H+	53% of the rate with 1-(4-nitrophenyl)ethanone, 78% yield, 98% enantiomeric excess	Ogataea glucozyma	(1S)-1-(2-nitrophenyl)ethanol + NADP+	-	?	438617	
1.1.1.320	1-(3-fluorophenyl)ethanone + NADPH + H+	61% of the rate with 1-(4-nitrophenyl)ethanone, 85% yield, 98% enantiomeric excess	Ogataea glucozyma	(1S)-1-(3-fluorophenyl)ethanol + NADP+	-	?	438618	
1.1.1.320	1-(4-fluoro-phenyl)-2-phenyl-ethane-1,2-dione + NADPH + H+	-	Bacillus cereus	? + NADP+	-	?	418196	
1.1.1.320	1-(4-methyl-phenyl)-2-phenyl-ethane-1,2-dione + NADPH + H+	-	Bacillus cereus	? + NADP+	-	?	418197	
1.1.1.320	1-(4-nitrophenyl)ethanone + NADPH + H+	100% yield, 98% enantiomeric excess	Ogataea glucozyma	(1S)-1-(4-nitrophenyl)ethanol + NADP+	-	?	438619	
1.1.1.320	1-(furan-2-yl)ethanone + NADPH + H+	-	Ogataea glucozyma	(1S)-1-(furan-2-yl)ethanol + NADP+	48% of the activity with benzil, 95% yield, 98% enantiomeric excess	?	438620	
1.1.1.320	1-(pyridin-3-yl)ethanone + NADPH + H+	-	Ogataea glucozyma	(S)-(1-pyridin-3yl)ethanol + NADP+	58% of the activity with benzil, 95% yield, 98% enantiomeric excess	?	438621	
1.1.1.320	1-(pyridin-3-yl)propan-1-one + NADPH + H+	-	Ogataea glucozyma	(S)-1-(pyridin-3-yl)propanol + NADP+	60% of the activity with benzil, 95% yield, 98% enantiomeric excess	?	438622	
1.1.1.320	1-(thiophen-2-yl)ethanone + NADPH + H+	-	Ogataea glucozyma	(1S)-1-(thiophen-2-yl)ethanol + NADP+	51% of the activity with benzil, 95% yield, 98% enantiomeric excess	?	438623	
1.1.1.320	1-phenyl-1,2-propanedione + NADPH + H+	-	Bacillus cereus	? + NADP+	-	?	418202	
1.1.1.320	2-acetylbenzonitrile + NADPH + H+	82% of the rate with 1-(4-nitrophenyl)ethanone, 98% yield, 98% enantiomeric excess	Ogataea glucozyma	2-[(1S)-1-hydroxyethyl]benzonitrile + NADP+	-	?	438708	
1.1.1.320	2-hydroxy-1-phenyl-1-propanone + NADP+	-	Bacillus cereus	1-phenylpropane-1,2-dione + NADPH + H+	-	?	422403	
1.1.1.320	3-oxo-3-phenylpropanenitrile + NADPH + H+	-	Ogataea glucozyma	(3S)-3-hydroxy-3-phenylpropanenitrile + NADP+	55% of the activity with benzil, 95% yield, 95% enantiomeric excess	?	438807	
1.1.1.320	4-acetylbenzonitrile + NADPH + H+	68% of the rate with 1-(4-nitrophenyl)ethanone, 70% yield, 97% enantiomeric excess	Ogataea glucozyma	4-[(1S)-1-hydroxyethyl]benzonitrile + NADP+	-	?	438824	
1.1.1.320	benzil + NADPH + H+	-	Ogataea glucozyma	(S)-benzoin + NADP+	-	r	439246	
1.1.1.320	benzil + NADPH + H+	-	Ogataea glucozyma	(S)-benzoin + NADP+	98% enantiomeric excess	?	439246	
1.1.1.320	benzophenone + NADPH + H+	-	Ogataea glucozyma	diphenylmethanol + NADP+	66% yield	?	439247	
1.1.1.320	ethyl 2-oxo-2-phenylacetate + NADPH + H+	-	Ogataea glucozyma	ethyl (2S)-hydroxy(phenyl)acetate + NADP+	115% yield, 40% enantiomeric excess	?	439520	
1.1.1.320	additional information	the enzyme is also active with 1,4-naphthoquinone, dichlone, and 4-phenylbenzaldehyde, substrate specificity, overview. 1-acenaphthenol is oxidized, although the kcat/Km ratio is low. No activity with progesterone, daunorubicin, and menaquinone. No or poor activity with benzyl phenyl ketone, benzophenone, dibenzoylmethane, chalcone, 1-phenyl-1,3-butanedione, diacetyl, 3,4-hexanedione, and acetophenone	Bacillus cereus	?	-	?	89	
1.1.1.320	additional information	for the enantioselective reduction of mono-substituted acetophenones, reaction rates of meta- and para-derivatives are consistent with the electronic effects described by delta-Hammett coefficients. Enantioselectivity is determined by an opposite orientation of the substrate in the binding pocket. Reduction of ortho-derivatives occurs only with substrates bearing substituents with low steric impact, and reactivity is controlled by stereoelectronic features	Ogataea glucozyma	?	-	?	89	
1.1.1.320	additional information	the enzyme prefers space-demanding substrates, which are often converted with high stereoselectivity	Ogataea glucozyma	?	-	?	89	
1.1.1.320	additional information	the enzyme is preferentiallly active on aromatic 1,2-diketones. It catalyzes the stereoselective monoreduction and desymmerization of bulky dicarbonyls. Mechanism and in silico prediction of substrates reactivity, overview	Ogataea glucozyma	?	-	-	89	
1.1.1.320	sec-phenethyl alcohol + NADP+	-	Bacillus cereus	1-phenylethanone + NADPH + H+	-	?	419067