1.1.1.267 (3R,4S)-dihydroxy-5-oxohexylphosphonic acid + NADPH - Synechocystis sp. ? + NADP+ - ? 371275 1.1.1.267 1,2-dideoxy-D-threo-3-hexulose 6-phosphate + NADPH + H+ i.e. 1-methyl-1-deoxy-D-xylulose 5-phosphate or M-DXP, a substrate analogue whichis inactive with the wild-type but active with mutant W204F Synechocystis sp. 2-C-ethyl-D-erythritol 4-phosphate + NADP+ - ? 386942 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ - Hevea brasiliensis 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ - ? 386990 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ the forward reaction is preferred Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ - r 386990 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ NADPH is the preferred cofactor Plectranthus barbatus 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ - ? 386990 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ inverse secondary kinetic isotope effects for both 3-[2H] and 4-[2H]-D-1-deoxyxylulose-5-phosphate during DXR-catalyzed isomerization indicates that the enzyme operates through a retro-aldol/aldol mechanism Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ - ? 386990 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH - synthetic construct ? + NADP+ - ? 403223 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ir 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Pseudomonas aeruginosa 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ir 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ir 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Zymomonas mobilis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ir 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Mentha x piperita 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ir 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Catharanthus roseus 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Francisella tularensis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Digitalis purpurea 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Toxoplasma gondii 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - synthetic construct 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Ginkgo biloba 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Hevea brasiliensis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Thermotoga maritima 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Wurfbainia villosa 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Yersinia pestis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Francisella tularensis subsp. novicida 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Vibrio vulnificus 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Populus trichocarpa 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Dioscorea zingiberensis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Mycolicibacterium smegmatis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Centella asiatica 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Thymus vulgaris 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Artemisia annua 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Oryza sativa Japonica Group 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Santalum album 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ involved in the biosynthesis of isopentenylphosphate Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ key enzyme in the bacterial nonmevalonate pathway to terpenoids, DXR introduces the key branching structure required for the eventual production of isopentenyl diphosphate Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second committed step in isopentenyl diphosphate biosynthesis via the plastidial nonmevalonate pathway of isoprenoid biosynthesis, overview Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate Arabidopsis thaliana 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate, overview Plasmodium falciparum 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the methylerythritol 4-phosphate pathway Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ step in the methylerythritol 4-phosphate pathway Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview Plectranthus barbatus 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the enzyme catalyzes the second committed step in the 2C-methyl-D-erythritol 4-phosphate pathway for biosynthesis of isopentenyl diphosphate Ginkgo biloba 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Escherichia coli 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Vibrio vulnificus 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the forward direction is preferred Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ NADPH and 2-C-methyl-D-erythritol 4-phosphate bind to the free enzyme and the two bind together to generate a nonproductive ternary complex Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Francisella tularensis subsp. novicida U112 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Yersinia pestis A1122 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Mycolicibacterium smegmatis ATCC 700084 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Vibrio vulnificus CMCP6 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Vibrio vulnificus CMCP6 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ first committed step in the mevalonate-independent isopentenyl diphosphate biosynthetic pathway, overview Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the forward direction is preferred Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+ - r 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate Mycobacterium tuberculosis H37Rv 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ - Mycobacterium tuberculosis ATCC 25618 2-C-methyl-D-erythritol 4-phosphate + NADP+ - ? 187985 1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADH - Mycobacterium tuberculosis methyl-D-erythritol-4-phosphate + NAD+ - r 371486 1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADPH - Escherichia coli methyl-D-erythritol-4-phosphate + NADP+ - ? 371487 1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADPH - Escherichia coli methyl-D-erythritol-4-phosphate + NADP+ - r 371487 1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADPH - Mycobacterium tuberculosis methyl-D-erythritol-4-phosphate + NADP+ - r 371487 1.1.1.267 1-deoxy-D-xylulose-5-phosphate + NADPH - Synechocystis sp. methyl-D-erythritol-4-phosphate + NADP+ - ? 371487 1.1.1.267 1-deoxy-L-erythrulose + NADPH + H+ reaction of the truncated substrate 1-deoxy-L-erythrulose in the presence and absence of phosphite dianion, overview Mycobacterium tuberculosis ? + NADP+ - ? 425265 1.1.1.267 1-fluoro-1-deoxy-D-xylulose 5-phosphate + NADPH i.e. CF-DXP Escherichia coli ? - ? 387000 1.1.1.267 1-fluoro-1-deoxy-D-xylulose 5-phosphate + NADPH i.e. CF-DXP, results in about 25% of the activity with 1-deoxy-D-xylulose 5-phosphate Escherichia coli ? - r 387000 1.1.1.267 1-fluoro-1-deoxy-D-xylulose-5-phosphate + NADPH - Escherichia coli ? + NADP+ - ? 371489 1.1.1.267 2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ in reaction mixtures containing 0.15-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle Mycobacterium tuberculosis ? + NADPH - r 403653 1.1.1.267 2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ in reaction mixtures containing 0.15-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle Escherichia coli ? + NADPH - r 403653 1.1.1.267 2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ in reaction mixtures containing 0.15–0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle Arabidopsis thaliana ? + NADPH - r 403653 1.1.1.267 2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Arabidopsis thaliana ? + NADPH - ? 403654 1.1.1.267 2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Mycobacterium tuberculosis ? + NADPH - ? 403654 1.1.1.267 2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Escherichia coli ? + NADPH - ? 403654 1.1.1.267 3-[2H]-D-1-deoxyxylulose-5-phosphate + NAD(P)H - Escherichia coli ? + NAD(P)+ - ? 395782 1.1.1.267 4-[2H]-D-1-deoxyxylulose-5-phosphate + NAD(P)H - Escherichia coli ? + NAD(P)+ - ? 395967 1.1.1.267 glycolaldehyde phosphate + hydroxyacetone + NADPH reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Arabidopsis thaliana ? + NADP+ - ? 405327 1.1.1.267 glycolaldehyde phosphate + hydroxyacetone + NADPH reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Mycobacterium tuberculosis ? + NADP+ - ? 405327 1.1.1.267 glycolaldehyde phosphate + hydroxyacetone + NADPH reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Escherichia coli ? + NADP+ - ? 405327 1.1.1.267 methyl-D-erythritol-4-phosphate + NADP+ - Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate + NADPH - r 373837 1.1.1.267 additional information participation in the control of the 2-C-methyl-D-erythritol 4-phosphate pathway Arabidopsis thaliana ? - ? 89 1.1.1.267 additional information reaction depends on NADPH although it does not involve a reduction step, putative aldehyde intermediate is converted to methyl-D-erythritol-4-phosphate in the presence of NADPH and to 1-deoxy-D-xylolose-5-phosphate in the presence of NADP+ Escherichia coli ? - ? 89 1.1.1.267 additional information 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid, 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid, and 1,2-dideoxy-D-hexulose 6-phosphate are poor substrates Escherichia coli ? - ? 89 1.1.1.267 additional information substrate synthesis and product identification method, overview Synechocystis sp. ? - ? 89 1.1.1.267 additional information a retroaldol/aldol mechanism for the DXR catalyzed rearrangement of 1-deoxy-D-xylulose 5-phosphate to 2-C-methyl-D-erythritol 4-phosphate. DXR first cleaves the C3-C4 bond of 1-deoxy-D-xylulose 5-phosphate in a retroaldol manner to generate a three-carbon and a two-carbon phosphate bimolecular intermediate. These two species are then reunited by an aldol reaction to form a new C-C bond, yielding the same aldehyde intermediate methylerythrose phosphate. Subsequent reduction of methylerythrose phosphate by NADPH affords 2-C-methyl-D-erythritol 4-phosphate synthetic construct ? - ? 89 1.1.1.267 additional information DXR can accelerate the biosynthesis of lycopene. DXR is an effective regulatory site for the synthesis and accumulation of diterpenoid compounds such as tanshinone Salvia miltiorrhiza ? - ? 89 1.1.1.267 additional information DXR has rate-limiting roles in isopentenyl diphosphate and dimethylallyl diphosphate synthesis Hevea brasiliensis ? - ? 89 1.1.1.267 additional information DXR is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway Pinus densiflora ? - ? 89 1.1.1.267 additional information IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206-216) is able to fold into at least three different conformations Mycobacterium tuberculosis ? - ? 89 1.1.1.267 additional information IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206-216) is able to fold into at least three different conformations Escherichia coli ? - ? 89 1.1.1.267 additional information IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206–216) is able to fold into at least three different conformations Arabidopsis thaliana ? - ? 89 1.1.1.267 additional information mono-((2S,3S)-3-fluoromethyl-2,4-dihydroxy-3-methyl-butyl) ester is not a substrate for DXR synthetic construct ? - ? 89 1.1.1.267 additional information used for the synthesis of [1-13C1]2C-methyl-D-erythritol 4-phosphate and [3-13C1]2C-methyl-D-erythritol 4-phosphate Bacillus subtilis ? - ? 89 1.1.1.267 additional information development and evaluation of a high-throughput screening assay for simultaneous selection of inhibitors of the enzyme, overview Mycobacterium tuberculosis ? - ? 89 1.1.1.267 additional information C3-C4 substrate binding mode in which DXP chelates a DXR-bound divalent cation via its hydroxyl groups at C3 and C4. The conversion of 1-deoxy-D-xylulose 5-phosphate to 2-methyl-D-erythritol 4-phosphate max include a pseudo-single molecule transition state of the retro-aldol intermediates Escherichia coli ? - ? 89 1.1.1.267 additional information product identification by mass spectrometry (HPLC-MS) Populus trichocarpa ? - - 89 1.1.1.267 additional information VvDxr residues S186, H209, S222, N227 and K228 may participate in binding of the DXP substrate's phosphate moiety. These residues are conserved. H209 is a part of a flexible loop (209-HPXWXMG-115) that closes on the active site upon substrate binding. While H209 interacts with the phosphate group, W211 and M213 shield the remaining part of Dxp from solvent Vibrio vulnificus ? - - 89 1.1.1.267 additional information C3-C4 substrate binding mode in which DXP chelates a DXR-bound divalent cation via its hydroxyl groups at C3 and C4. The conversion of 1-deoxy-D-xylulose 5-phosphate to 2-methyl-D-erythritol 4-phosphate max include a pseudo-single molecule transition state of the retro-aldol intermediates Escherichia coli O157 ? - ? 89 1.1.1.267 additional information VvDxr residues S186, H209, S222, N227 and K228 may participate in binding of the DXP substrate's phosphate moiety. These residues are conserved. H209 is a part of a flexible loop (209-HPXWXMG-115) that closes on the active site upon substrate binding. While H209 interacts with the phosphate group, W211 and M213 shield the remaining part of Dxp from solvent Vibrio vulnificus CMCP6 ? - - 89