6.5.1.3	additional information	the enzyme seals nicked 3'-OH/5'-PO4 duplexes in which the 3'-OH strand is RNA. The enzyme entails reaction with ATP to form a covalent enzyme-AMP intermediate, transfer of AMP to the nick 5'-PO4, and attack of the RNA 3'-OH on the adenylylated nick to form a 3'-5' phosphodiester	Naegleria gruberi	?	-	?	89	
6.5.1.3	ATP + (2E,6E)-farnesyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	(2E,6E)-farnesyl-triphospho(5')adenosine + diphosphate	-	ir	411651	
6.5.1.3	ATP + (2E,6E)-farnesyl triphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	(2E,6E)-farnesyl-tetraphospho(5')adenosine + diphosphate	-	ir	411652	
6.5.1.3	ATP + 5'-aaaCCAGUC	RNA-DNA hybrid as substrate, chemical step is represented as irreversible, because reverse reaction, pyrophosphorolysis with formation of a triphosphate is 10000000-times slower than the forward reaction	synthetic construct	AMP + diphosphate + ?	-	ir	369204	
6.5.1.3	ATP + dimethylallyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	dimethylallyl-triphospho(5')adenosine + diphosphate	-	ir	411667	
6.5.1.3	ATP + geranyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	geranyl-triphospho(5')adenosine + diphosphate	-	ir	411671	
6.5.1.3	ATP + geranyl triphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	geranyl-tetraphospho(5')adenosine + diphosphate	-	ir	411673	
6.5.1.3	ATP + isopentenyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	adenosine 5'-isopentenyl triphosphate + diphosphate	-	ir	411675	
6.5.1.3	ATP + isopentenyl triphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	isopentenyl-tetraphospho(5')adenosine + diphosphate	-	ir	411678	
6.5.1.3	ATP + (ribonucleotide)n + (ribonucleotide)m	-	Tequatrovirus T4	AMP + diphosphate + (ribonucleotide)n+m	-	r	813