6.2.1.76 ATP + 2-methylmalonate + CoA activity is 53.6% compared to the activity with malonate AMP + diphosphate + 2-methyl-malonyl-CoA - ? 462276 6.2.1.76 ATP + 2-methylmalonate + CoA activity is 20.4% compared to the activity with malonate AMP + diphosphate + 2-methylmalonyl-CoA - ? 462277 6.2.1.76 ATP + 2-methylmalonate + CoA activity with 2-methylmalonate is 12% compared to the activity malonate AMP + diphosphate + 2-methylmalonyl-CoA - ? 462277 6.2.1.76 ATP + malonate + CoA - AMP + diphosphate + malonyl-CoA - ? 461531 6.2.1.76 ATP + malonate + CoA high substrate specificity on malonate and ATP AMP + diphosphate + malonyl-CoA - ? 461531 6.2.1.76 ATP + malonate + CoA ordered bi uni uni bi ping pong ter ter mechanism AMP + diphosphate + malonyl-CoA - ? 461531 6.2.1.76 ATP + malonate + CoA with activity with acetate, propionate, or succinate in place of malonate, or with GTP or CTP in place of ATP AMP + diphosphate + malonyl-CoA - ? 461531 6.2.1.76 ATP + malonate + CoA no activity with oxalate, succinate, butyrate, octanoate, laurate, palmitate, oleate, or lignocerate AMP + diphosphate + malonyl-CoA - ? 461531 6.2.1.76 ATP + malonate + CoA little or no activity against other dicarboxylic or fatty acids AMP + diphosphate + malonyl-CoA - ? 461531 6.2.1.76 ATP + methylmalonate + CoA the kcat/KM value for methylmalonate is 12.6fold lower than the kcat/Km value for malonate AMP + diphosphate + methylmalonyl-CoA - ? 462281 6.2.1.76 ATP + methylmalonate + CoA 70.3% of the activity compared to malonate AMP + diphosphate + methylmalonyl-CoA - ? 462281 6.2.1.76 additional information no activity for acetate, propionate and succinate in place of malonate, and for GTP, CTP and AMP in place of ATP ? - ? 89