3.5.5.4 additional information no substrates are various nitrile or amide compounds, overview Pseudomonas sp. 13 ? - ? 89 3.5.5.4 additional information recombinant NIT4 isozymes NIT4A, NIT4B1, and NIT4B2 do not hydrolyze 3-cyano-L-alanine, benzonitrile, 4-hydroxybenzonitrile, butyronitrile, and 4-pentenenitrile Sorghum bicolor ? - ? 89 3.5.5.4 beta-cyano-L-alanine + H2O the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate Lotus japonicus L-aspartate + NH3 - ? 414370 3.5.5.4 beta-cyano-L-alanine + H2O the reaction is catalyzed only by isoform NIT4A, L-asparagine is formed as an intermediate Sorghum bicolor L-aspartate + NH3 - ? 414370