1.2.1.29 4-hydroxybenzaldehyde + NAD+ + H2O - Phanerodontia chrysosporium 4-hydroxybenzoic acid + NADH + H+ - ? 414110 1.2.1.29 aromatic aldehydes + NAD+ + H2O - Oryctolagus cuniculus aromatic acids + NADH - ? 369992 1.2.1.29 benzaldehyde + NAD+ + H2O - Phanerodontia chrysosporium benzoic acid + NADH + H+ - ? 425997 1.2.1.29 gentisaldehyde + NAD+ - Oryctolagus cuniculus gentisic acid + NADH - ? 189184 1.2.1.29 vanillin + NAD+ - Trametes cinnabarina vanillinic acid + NADH + H+ - r 440420 1.2.1.29 vanillin + NAD+ - Trametes cinnabarina MUCL 39533 vanillinic acid + NADH + H+ - r 440420 1.2.1.29 4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O 4-hydroxy-3-methoxybenzaldehyde i.e. vanillin Phanerodontia chrysosporium 4-hydroxy-3-methoxybenzoic acid + NADH + H+ - ? 414109 1.2.1.29 additional information ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues Trametes cinnabarina ? - ? 89 1.2.1.29 additional information ALDH catalysis involves acylation and deacylation. During acylation, a cysteine nucleophile interacts with the carbonyl carbon of aldehyde forming a thiohemiacetal intermediate, followed by hydride transfer from a tetrahedral thiohemiacetal intermediate to the pyridine ring of NAD(P)+. Then, deacylation occurs involving hydrolysis of the resulting thioester intermediate. Glu268 and Cys296 of PcALDH are potential active site residues Trametes cinnabarina MUCL 39533 ? - ? 89 1.2.1.29 additional information the enzyme is capable of catalysing the oxidation of a number of aromatic aldehydes, but not aliphatic aldehydes Oryctolagus cuniculus ? - ? 89