1.14.99.53 alpha-chitin + acceptor + O2 - oligosaccharide aldonic acids + reduced acceptor + H2O - ? 439105 1.14.99.53 alpha-chitin + ascorbate + O2 - ? + dehydroascorbate + H2O products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom ? 439106 1.14.99.53 alpha-chitin + ascorbate + O2 - ? + dehydroascorbate + H2O - ? 439106 1.14.99.53 alpha-chitin + ascorbate + O2 enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products show oxidation of the C1 carbon leading to aldonic acids ? 439107 1.14.99.53 alpha-chitin + ascorbate + O2 - C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products are C1-oxidized chitooligomers, mainly tetramers and hexamers ? 439107 1.14.99.53 alpha-chitin + ascorbate + O2 - chito-oligosaccharide aldonic acid + dehydroascorbate + H2O products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation ? 439108 1.14.99.53 alpha-chitin + ascorbate + O2 - oxidized chitooligosaccharides + dehydroascorbate + H2O - ? 439109 1.14.99.53 alpha-chitin + ascorbate + O2 - oxidized oligosaccharides + dehydroascorbate + H2O - ? 439110 1.14.99.53 alpha-chitin + gallate + O2 - ? + H2O - ? 463877 1.14.99.53 alpha-chitin + gallate + O2 - chito-oligosaccharide + C1-aldonic acids + ? + H2O - ? 463878 1.14.99.53 alpha-chitin + gallate + O2 - chito-oligosaccharide C1-aldonic acids + dehydroascorbate + H2O - ? 463879 1.14.99.53 beta-chitin + acceptor + O2 - chito-oligosaccharide aldonic acid + reduced acceptor + H2O products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation ? 439253 1.14.99.53 beta-chitin + acceptor + O2 beta chitin from squid pen, best substrate oligosaccharide aldonic acids + reduced acceptor + H2O strong preference towards even-numbered products ? 439254 1.14.99.53 beta-chitin + acceptor + O2 - oligosaccharide aldonic acids + reduced acceptor + H2O - ? 439254 1.14.99.53 beta-chitin + acceptor + O2 - chito-oligosaccharide aldonic acids + reduced acceptor + H2O - ? 464186 1.14.99.53 beta-chitin + ascorbate + O2 - ? + dehydroascorbate + H2O products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom ? 439255 1.14.99.53 beta-chitin + ascorbate + O2 - ? + dehydroascorbate + H2O - ? 439255 1.14.99.53 beta-chitin + ascorbate + O2 - C1-oxidized chitooligosaccharides + dehydroascorbate + H2O - ? 439256 1.14.99.53 beta-chitin + ascorbate + O2 enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids ? 439256 1.14.99.53 beta-chitin + ascorbate + O2 beta-chitin is preferred over alpha-chitin C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products are C1-oxidized chitooligomers, mainly tetramers and hexamers ? 439256 1.14.99.53 beta-chitin + ascorbate + O2 - C1-oxidized oligosaccharides + dehydroascorbate + H2O - ? 439257 1.14.99.53 beta-chitin + ascorbate + O2 substrate squid pen beta-chitin C1-oxidized oligosaccharides + dehydroascorbate + H2O in addition, considerable amounts of partially deacetylated oligomers are produced ? 439257 1.14.99.53 beta-chitin + ascorbate + O2 - oxidized oligosaccharides + dehydroascorbate + H2O - ? 439260 1.14.99.53 beta-chitin + ascorbic acid + O2 - ? + dehydroascorbate + H2O - ? 443242 1.14.99.53 beta-chitin + cellobiose dehydrogenase + O2 cellobiose dehydrogenase from Myriococcum thermophilum can act as an electron donor C1-oxidized oligosaccharides + reduced cellobiose dehydrogenase + H2O - ? 439261 1.14.99.53 beta-chitin + gallate + O2 - ? + H2O - ? 464187 1.14.99.53 cellulose + ascorbate + O2 - ? + dehydroascorbate + H2O - ? 439312 1.14.99.53 chitin + acceptor + O2 - [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone the products are a series of chitin oligosaccharides in aldonic acid or lactone form with varying degree of polymerization: DPox4 > DPox5 > DPox6 > DPox7 > DPox8. Oligosaccharides with even-numbered chain lengths are predominant. ? 435394 1.14.99.53 chitin + acceptor + O2 - ? + reduced acceptor + H2O - ? 439325 1.14.99.53 chitin + acceptor + O2 - C1-oxidized chito-oligosaccharides + reduced acceptor + H2O primary chain cleavage, yielding predominantly aldonic acid oligosaccharides with even-numbered degrees of polymerization plus significant presence of unmodified oligosaccharides with uneven-numbered degrees of polymerization ? 439326 1.14.99.53 chitin + ascorbate + O2 - C1-oxidized chito-oligosaccharides + dehydroascorbate + H2O - ? 464230 1.14.99.53 chitin + ascorbate + O2 - chito-oligosaccharide + dehydroascorbate + H2O - ? 464231 1.14.99.53 chitin + ascorbic acid + O2 - ? + dehydroascorbate + H2O - ? 443285 1.14.99.53 colloidal chitin + ascorbate + O2 - ? + dehydroascorbate + H2O low activity, products show less dominance of even-numbered products than alpha- or beta-chitin ? 439351 1.14.99.53 colloidal chitin + ascorbate + O2 - [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O - ? 439352 1.14.99.53 colloidal chitin + ascorbate + O2 - oxidized chitin oligosaccharides + dehydroascorbate + H2O with colloidal chitin as the substrate, a ladder of oxidized oligosaccharides is observed ? 439354 1.14.99.53 crystalline chitin + ascorbate + O2 - oxidized oligosaccharides + dehydroascorbate + H2O enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin ? 439360 1.14.99.53 additional information data indicate that catalysis involves equatorial binding of a reactive oxygen species ? - ? 89 1.14.99.53 additional information the enzyme produces oxidized chitin oligosaccharides with a degree of polymerization from DPox3 to DPox11. The relative intensities of DPox4, DPox6, DPox8 and DPox10 are remarkably higher than DPox5, DPox7, DPox9 and DPox11. LPMO10A shows little binding to Avicel ? - ? 89 1.14.99.53 additional information no activity on other substrates including diverse mannans, cellulose and starch ? - ? 89 1.14.99.53 additional information enzyme in presence of ascorbate but lacking chitin produces H2O2 ? - ? 89 1.14.99.53 additional information mechanistic model, copper is reduced on the enzyme by an externally provided electron and followed by oxygen binding and activation by internal electron transfer. Substrate binding involves an extended planar binding surface, including the metal binding site. Chitin binding protects two regions from 2H/1H exchange, Gln53-Ser58 and Leu110-Thr116 ? - ? 89 1.14.99.53 additional information isoform LPMO10B produces C4-oxidized (4-ketoaldoses) and double (C4/C1)-oxidized cello-oligosaccharides. No substrate: alpha-chitin ? - ? 89 1.14.99.53 additional information enzyme binds to cellulose, but does not display catalyic activity ? - ? 89 1.14.99.53 additional information the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved ? - ? 89 1.14.99.53 additional information [(1->4)-N-acetyl-beta-D-glucosaminyl]6 is a substrate, but not shorter oligomers ? - ? 89 1.14.99.53 additional information presence of ascorbate is required. No substrates: phosphoric acid-swollen cellulose, avicel, tamarind xyloglucan, birchwood xylan, beechwood xylan, acetyl glucuronoxylan from aspen, ivory nut mannan, acetylated konjac glucomannan, potato starch, heparin, hyaluronic acid, and chitosan ? - ? 89 1.14.99.53 additional information enzyme additionally shows peroxigenase activity. The peroxygenase reaction is faster than the monooxygenase reaction ? - ? 89 1.14.99.53 phosphoric acid swollen cellulase + ascorbic acid + O2 - ? + dehydroascorbate + H2O - ? 443845 1.14.99.53 phosphoric acid swollen cellulose + ascorbate + O2 reaction of EC 1.14.99.54 C4-oxidized cellooligosaccharides + C1/C4-oxidized cellooligosaccharides + dehydroascorbate + H2O - ? 439826 1.14.99.53 [(1->4)-N-acetyl-beta-D-glucosaminyl]6 + ascorbate + O2 - [(1->4)-N-acetyl-beta-D-glucosaminyl]3-(1->4)-N-acetyl-2-deoxy-2-amino-D-glucono-1,5-lactone + [(1->4)-N-acetyl-beta-D-glucosaminyl]2 + dehydroascorbate + H2O - ? 443984 1.14.99.53 [(1->4)-N-acetyl-beta-D-glucosaminyl]n+m + reduced acceptor + O2 - [(1->4)-N-acetyl-beta-D-glucosaminyl]m-1-(1->4)-N-acetyl-2-deoxy-2-amino-D-glucono-1,5-lactone + [(1->4)-N-acetyl-beta-D-glucosaminyl]n + acceptor + H2O - ? 440005