1.5.1.5 -17°C, partially purified enzyme after second ammonium sulphate fractionation stable for 2 weeks, 1 mM mercaptoethanol, 1.5.1.5 -20°C, 0.1% bovine plasma albumin 1.5.1.5 -20°C, 1 month, residual activity of 50% 1.5.1.5 -20°C, purified MTHFD2L in 25 mM Tris-Cl, 10 mM 2-mercaptoethanol, 100mM KCl, and 50% v/v glycerol at pH 7.5. The purified enzyme retains about 75% of initial 5,10-CH2THF dehydrogenase activity after 1 week of storage 1.5.1.5 -20°C, stable for at least 2 months at diluted protein concentration 1.5.1.5 -20°C, stable fot at least 2 months 1.5.1.5 0-4°C, after 1 month retains 75% of the methylenetetrahydrofolate dehydrogenase activity 1.5.1.5 10% glycerol required to prevent loss of activity during storage 1.5.1.5 2-4°C, 10% loss of activity in 4 days 1.5.1.5 4°C, in the presence of 25% glycerol, 1 month, residual activity of 85%