3.5.4.5 -20°C crude enzyme is stable for 4 weeks, repeated freezing and thawing destroy the activity 3.5.4.5 -20°C, 0.005 M dithiothreitol, enzymatic activity is stable for many weeks 3.5.4.5 -20°C, 20 mM Tris-HCl, pH 7.5, 1 mM DDT, 1 mM EDTA, 20% glycerol, enzyme retains full activity for at least 1 month, 80% of activity lost in absence of glycerol 3.5.4.5 -20°C, 20% glycerol appears to be necessary to prevent inactivation from occuring after freezing and thawing 3.5.4.5 -20°C, 50 mM phosphate buffer pH7.5 after 1 month pure enzyme retains 70% original activity 3.5.4.5 -20°C, 50 mM Tris-HCl, pH7.5, after 1 month pure enzyme retains 80% original activity 3.5.4.5 -20°C, 50% glycerol, very stable, litle loss of enzymatic activity after 1 year 3.5.4.5 -20°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h 3.5.4.5 -20°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h 3.5.4.5 -20°C, preparations kept 4 months retains original activity 3.5.4.5 -20°C, shows no appreciable loss of activity within 1 month 3.5.4.5 4°C, 50 mM Tris-HCl, pH 7.5, pure enzyme retains 63% original activity after 2 weeks 3.5.4.5 4°C, enzyme lost about 50% activity after 24 h, essentially all activity lost after 48 h 3.5.4.5 4°C, enzyme lost about 50% activity after 24 h, no activity remains after 24 h 3.5.4.5 4°C, in presence of 5 mM mercaptoethanol, reasonably stable, retains 47% of its activity after 3 months, freezing inactivates the enzyme, less than 30% recovery of activity after 1 freeze-thaw cycle 3.5.4.5 4°C, reasonably stable, retains 55% of its activity after 3 weeks 3.5.4.5 4°C, stable for several days, may be kept for months without significant loss of activity if frozen 3.5.4.5 4°C, stable for some hours at pH 5.5-9.5 3.5.4.5 4°C, very unstable, 50% of activity lost on storage for 24 h or 24 h at room temperature 3.5.4.5 4°C,50 mM phosphate buffer pH 7.5, pure enzyme retains 13% original activity after 2 weeks