1.13.12.6 -15°C, distilled water 1.13.12.6 -80°C, lyophilized Cypridina luciferin is stable for more than 1 year and the enzyme in acidic ethanol solution for at least 1 month 1.13.12.6 4°C, PBS buffer containing 0.1 M potassium phosphate (pH 7.2) and 0.15 M NaCl, 2 months, without significant loss of activity 1.13.12.6 Cypridina luciferase occasionally loses almost all of its activity when chromatographed through the 90-cm Sephadex column, irrespective of whether the column is newly poured or has been used for a period. The loss also occurs with both newly dissolved and old solutions of Cypridina luciferase 1.13.12.6 purified native Apogon luciferase gradually loses activity on standing in the cold over a period of weeks