4.2.2.3 additional information - - 34062, 34064, 34068, 34080, 651661 4.2.2.3 additional information - activity of different enzyme expressing plasmid constructs in Escherichia coli 651668 4.2.2.3 additional information - assay methods, overview 648532 4.2.2.3 additional information - assay methods, overview, endolytic activity 648532 4.2.2.3 additional information - the specific activity of the recombinant enzyme at 30°C in buffer containing 10 mM NaPi (pH 8.0) is 2490 units/mg, one unit of lyase activity is defined as the amount of enzyme that increases the absorbance at 235 nm by 0.01 unit after 1 min 705483 4.2.2.3 0.01 - substrate poly(alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C 748221 4.2.2.3 0.021 - purified recombinant enzyme 651347 4.2.2.3 0.0274 - protein PA1167 665543 4.2.2.3 0.03 - substrate poly(alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C 748221 4.2.2.3 0.08 - substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C 748221