2.6.1.16 additional information - development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format 684404 2.6.1.16 0.001 - mutant enzyme L468P 672444 2.6.1.16 0.0047 - - 639918 2.6.1.16 0.00783 - - 639921 2.6.1.16 0.013 - - 639905 2.6.1.16 0.0144 - - 639923 2.6.1.16 0.021 - glutamine 6-phosphate-synthetic activity, mutant V711F, pH 7.5, 25°C 671896 2.6.1.16 0.05 - glutamine 6-phosphate-synthetic activity, mutant W97G, pH 7.5, 25°C 671896 2.6.1.16 0.095 - amidohydrolysing activity of the mutant enzyme W97G using D-fructose 6-phosphate as substrate 671896 2.6.1.16 0.16 - - 639919