2.2.1.6 additional information - - 395915, 395919, 395922, 672368, 673744 2.2.1.6 additional information - assay methods 395917 2.2.1.6 additional information - effects of pyruvate and sucrose on relative ALS activity in light and dark conditions, overview 701404 2.2.1.6 additional information - isozyme substrate specificities 671290 2.2.1.6 additional information - specific activity also depends on protein concentration, with lower concentration giving lower activity, activity may be generally increased by pre-incubation at 30°C prior to assay 658566 2.2.1.6 additional information - spectrophotometric assay involving an indirect detection of the product acetolactate 395872 2.2.1.6 additional information - time lag during the production of acetolactate from pyruvate and cofactors. Time lag is temperature depended being lowest at 37°C with 250 seconds 658365 2.2.1.6 0.0023 - pH 7.5, 37°C, purified recombinant His6-tagged mutant E85A 733747 2.2.1.6 0.0043 - substrate: pyruvate, pH 7.5, 37°C, in the presence of 3.5 M KCl 727336 2.2.1.6 0.03 - mutant H351Q, 37°C, pH 7.5 657720