5.4.99.5 additional information - - 3557, 3574 5.4.99.5 additional information - Activation Energies in the enzyme reactions by AMBER and FMO calculations for wild-type and mutants are shown. These results are potential energy, not free energy 675611 5.4.99.5 additional information - enzyme activity in presence of amino acid effectors, overview 747032 5.4.99.5 0.198 - - 3541 5.4.99.5 0.44 - cell-free supernatant 706137 5.4.99.5 1.2 - - 662365 5.4.99.5 21.7 - - 3554 5.4.99.5 25 - after affinity chromatography 649515 5.4.99.5 36.63 - - 3542 5.4.99.5 48.6 - after 110.4fold purification 706137 5.4.99.5 52 - - 3543 5.4.99.5 74 - - 662365 5.4.99.5 121 - - 3540 5.4.99.5 168 - Superdex 200pg pool 651660 5.4.99.5 200 - - 3562 5.4.99.5 210 - recombinant enzyme 3562 5.4.99.5 300 - - 3555 5.4.99.5 1200 - 0.1 mM tyrosine, wild-type 651936 5.4.99.5 3300 - 0.1 mM tyrosine, mutant T226D 651936 5.4.99.5 3700 - unliganded, mutant T226D 651936 5.4.99.5 4800 - unliganded, wild-type 651936 5.4.99.5 8400 - 0.1 mM tyrosine, wild-type 651936 5.4.99.5 11100 - 0.5 mM tryptophan, mutant T226D 651936 5.4.99.5 13400 - 0.1 mM tyrosine, mutant D233I 651936 5.4.99.5 20600 - unliganded, mutant T226I 651936 5.4.99.5 21700 - 0.1 mM tyrosine, mutant T226I 651936 5.4.99.5 22000 - 0.1 mM tyrosine, mutant D233T 651936 5.4.99.5 26400 - 0.5 mM tryptophan, mutant T226I 651936 5.4.99.5 30300 - unliganded, mutant D233I 651936 5.4.99.5 32500 - unliganded, wild-type 651936 5.4.99.5 40200 - 0.5 mM tryptophan, wild-type 651936 5.4.99.5 41300 - unliganded, mutant D233T 651936 5.4.99.5 63000 - 0.5 mM tryptophan, mutant D233I 651936 5.4.99.5 64300 - 0.5 mM tryptophan, mutant D233T 651936 5.4.99.5 88500 - 0.5 mM tryptophan, wild-type 651936