4.2.2.3 additional information - - 34062, 34064, 34068, 34080, 651661 4.2.2.3 additional information - activity of different enzyme expressing plasmid constructs in Escherichia coli 651668 4.2.2.3 additional information - assay methods, overview 648532 4.2.2.3 additional information - assay methods, overview, endolytic activity 648532 4.2.2.3 additional information - the specific activity of the recombinant enzyme at 30°C in buffer containing 10 mM NaPi (pH 8.0) is 2490 units/mg, one unit of lyase activity is defined as the amount of enzyme that increases the absorbance at 235 nm by 0.01 unit after 1 min 705483 4.2.2.3 0.01 - substrate poly(alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C 748221 4.2.2.3 0.021 - purified recombinant enzyme 651347 4.2.2.3 0.0274 - protein PA1167 665543 4.2.2.3 0.03 - substrate poly(alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C 748221 4.2.2.3 0.08 - substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant G118N, pH 7.0, 30°C 748221 4.2.2.3 0.11 - substrate alginate, mutant Y142F, pH 7.0, 30°C 748221 4.2.2.3 0.142 - crude extract, at pH 10.0 703603 4.2.2.3 0.16 - substrate poly(alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C 748221 4.2.2.3 0.18 - substrate poly(beta-(1,4)-D-mannuronate), mutant G118N, pH 7.0, 30°C 748221 4.2.2.3 0.22 - substrate alginate, mutant Y140F, pH 7.0, 30°C 748221 4.2.2.3 0.31 - substrate alginate, mutant G118N, pH 7.0, 30°C 748221 4.2.2.3 0.83 - substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), mutant C115A/C124G, pH 7.0, 30°C 748221 4.2.2.3 0.926 - AlgL 665543 4.2.2.3 1.14 - wild-type, pH 7.5, 30°C 704682 4.2.2.3 1.98 - wild-type enzyme, pH 7.0, 50°C, substrate alginate 729459 4.2.2.3 2.2 - substrate poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate), wild-type, pH 7.0, 30°C 748221 4.2.2.3 2.86 - - 34063 4.2.2.3 6 - substrate alginate, mutant C115A/C124G, pH 7.0, 30°C 748221 4.2.2.3 6.8 - substrate poly(beta-(1,4)-D-mannuronate), mutant C115A/C124G, pH 7.0, 30°C 748221 4.2.2.3 7.43 - substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 30°C 747942 4.2.2.3 12.31 - substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 30°C 747942 4.2.2.3 16.1 - - 665769 4.2.2.3 17.4 - substrate alginate, wild-type, pH 7.0, 30°C 748221 4.2.2.3 21.14 - substrate alginate, pH 7.0, 30°C 747942 4.2.2.3 21.48 - substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C 749227 4.2.2.3 21.77 - alginate lyase C, substrate with M blocks, pH 7.0, 30°C 713799 4.2.2.3 23.34 - substrate alginate, pH 8.0, 35°C 749227 4.2.2.3 27.03 - substrate poly(beta-(1,4)-D-mannuronate), pH 8.0, 35°C 749227 4.2.2.3 33 - substrate poly(beta-(1,4)-D-mannuronate), wild-type, pH 7.0, 30°C 748221 4.2.2.3 34.4 - purified enzyme 650305 4.2.2.3 41.4 - purified recombinant His-tagged enzyme, pH 7.0, 50°C, substrate alginate 729459 4.2.2.3 45 - purified isozyme A1-III 653728 4.2.2.3 67 - pH 6.5, 35°C 715322 4.2.2.3 67 - purified recombinant His-tagged enzyme, substrate poly(beta-D-mannuronate), pH 7.0, 50°C 729459 4.2.2.3 67.24 - purified enzyme, pH 6.5, 35°C 715322 4.2.2.3 73.1 - purified isozyme A1-I 653728 4.2.2.3 93.88 - purified enzyme 651639 4.2.2.3 108.5 - after 764.1fold purification, at pH 10.0 703603 4.2.2.3 109 - purified isozyme A1-II 653728 4.2.2.3 129 - alginate lyase C, substrate with G blocks, pH 7.0, 30°C 713799 4.2.2.3 222 - pH 8.0, 30°C 747385 4.2.2.3 330 - alginate lyase B, substrate with G blocks, pH 7.0, 30°C 713799 4.2.2.3 382 - alginate lyase B, substrate with M blocks, pH 7.0, 30°C 713799 4.2.2.3 382 - isoform B, substrate poly(beta-D-mannuronate), pH 7.0, 30°C 713799 4.2.2.3 390 - alginate lyase A, substrate with G blocks, pH 7.0, 30°C 713799 4.2.2.3 457 - pH 8.0, 30°C 747949 4.2.2.3 467 - isoform B, substrate sodium alginate, pH 7.0, 30°C 713799 4.2.2.3 644.6 - pH 8.0, 35°C 748341 4.2.2.3 651 - alginate lyase A, substrate with M blocks, pH 7.0, 30°C 713799 4.2.2.3 651 - isoform A, substrate poly(beta-D-mannuronate), pH 7.0, 30°C 713799 4.2.2.3 653 - isoform A, substrate sodium alginate, pH 7.0, 30°C 713799 4.2.2.3 937 - substrate poly(beta-D-mannuronic acid/alpha-L-guluronic acid), 22°C, pH 7.5 715553 4.2.2.3 1123 - extracellular enzyme, pH 7.0, 30°C 713799 4.2.2.3 1401 - pH 7.5, 30°C 705481 4.2.2.3 1487.6 - substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 50°C 747722 4.2.2.3 1620 - substrate poly(beta-1,4-D-mannuronate), pH 6.0, 70°C 748210 4.2.2.3 1786 - pH 7.0, 30°C 679049 4.2.2.3 2025 - wild-type, pH 6.0, 40°C 747854 4.2.2.3 2043 - substrate poly(beta-(1,4)-D-mannuronate), pH 7.0, 40°C 747945 4.2.2.3 2057 - pH 7.0, 30°C 714770 4.2.2.3 2057 - purified isozyme AkAly33, pH 8.0, 37°C 714770 4.2.2.3 2100 - purified recombinant His-tagged AkAly30, pH 8.0, 30°C 714373 4.2.2.3 2152 - purified enzyme, pH 7.0, 38°C 729470 4.2.2.3 2416 - substrate alginate, pH 7.0, 40°C 747945 4.2.2.3 2490 - 30°C, pH 8.0 705483 4.2.2.3 2604.9 - substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 50°C 747722 4.2.2.3 2920 - mutant T282N, pH 6.0, 40°C 747854 4.2.2.3 3343.7 - substrate alginate, pH 7.0, 50°C 747722 4.2.2.3 3359 - substrate poly(alpha-(1,4)-L-guluronate), pH 7.0, 40°C 747945 4.2.2.3 4803 - pH 8.5, 50°C 716092 4.2.2.3 4803 - purified enzyme, pH 8.5, 50°C 716092 4.2.2.3 5740 - pH 7.0, 30°C 714770 4.2.2.3 5741 - purified isozyme AkAly28, pH 8.0, 37°C 714770 4.2.2.3 5796 - purified native AkAly30, pH 8.0, 30°C 714373 4.2.2.3 7658 - substrate poly(beta-(1,4)-D-mannuronic acid), pH 7.5, 55°C 748532 4.2.2.3 7985 - substrate alginate, pH 7.5, 55°C 748532 4.2.2.3 8409 - purified native enzyme, pH 7.0, 50°C 729531 4.2.2.3 8643 - substrate poly(alpha-(1,4)-L-guluronic acid), pH 7.5, 55°C 748532