4.2.1.104	additional information	-	-	210672	
4.2.1.104	additional information	-	activity assay in 0.1 M potassium phosphate buffer at pH 7.5 and 37°C with or without 3 mM NaHCO3, cyanate concentration between 0.2 mM and 2 mM, 0.2 to 1 microgram total protein per milliliter, measurement of NH3 formation	678457	
4.2.1.104	additional information	-	cyanase activity shown in whole-cell extracts of Synechocystis, high level of cyanase activity when grown with nitrate as N source but unable to utilize exogenous cyanate to support O2 evolution in the light	680479	
4.2.1.104	additional information	-	cyanase activity shown in whole-cell extracts of the photoautotrophic Synechococcus sp. strain UTEX 625, ability to utilize cyanate-derived CO2 to support photosynthesis affected	680479	
4.2.1.104	additional information	-	strain 21 of Pseudomonas putida is a more active CN– decomposer than strain 18 of Pseudomonas stutzeri	681808	
4.2.1.104	0.01	-	in absence of bicarbonate	678457	
4.2.1.104	0.038	-	purified recombinant mutant E94L, pH 7.7, 27°C	716700	
4.2.1.104	0.04	-	purified recombinant mutant S117A, pH 7.7, 27°C	716700	
4.2.1.104	0.047	-	complemented deficient Escherichia coli cells	651632	
4.2.1.104	0.22	-	in the presence of 3 mM bicarbonate and 2 mM cyanate	678457	
4.2.1.104	0.303	-	purified recombinant enzyme, pH 4.8, 27°C	716700	
4.2.1.104	0.43	-	purified recombinant enzyme, pH 7.4, 55°C	713844	
4.2.1.104	0.633	-	purified recombinant enzyme, pH 5.7, 27°C	716700	
4.2.1.104	0.75	-	-	681808	
4.2.1.104	0.75	-	activity determined every 15 to 30 min in the presence of 2 mM KCNO and 2 mM HCO3- by formation of NH4+, absent activity if bacteria are grown with NH4 indicates inducible synthesis of cyanase	681808	
4.2.1.104	0.8	-	purified recombinant enzyme, pH 7.4, 4°C	713844	
4.2.1.104	1.26	-	-	681808	
4.2.1.104	1.26	-	pH optimum 8.1, activity determined every 15 to 30 min in the presence of 2 mM KCNO and 2 mM HCO3- by formation of NH4+, pH values of 7.0, 8.0 and 9.1, absent activity if bacteria are grown with NH4+ indicates inducible synthesis of cyanase	681808	
4.2.1.104	2.286	-	purified recombinant wild-type enzyme, pH 7.7, 27°C	716700	
4.2.1.104	5.56	-	purified recombinant enzyme, pH 7.4, 26°C	713844	
4.2.1.104	1666.7	-	measured by micromol ammonia/mg/s, transgenic Arabidopsis thaliana plants expressing the enzyme, pH and temperature not specified in the publication	747661