4.1.3.3	additional information	-	-	33314, 33316, 33319, 33321, 33325	
4.1.3.3	additional information	-	after a 36 h incubation, the concentrations of N-acetyl-D-neuraminic acid from the reactions utilizing 0.4, 0.8, 1.2, 1.6, 2.0, and 2.2 M pyruvate as the carbon source are 7.5 (3.75%), 26.9 (3.45%), 43.9 (21.95%), 56.0 (28%), and 61.9 mM (30.95%), respectively. When using 2.2 M pyruvate, in the first 6 h, the concentration of N-acetyl-D-neuraminic acid reaches 29.5 mM (which is about half of the maximal concentration), and the productivity of N-acetyl-D-neuraminic acid is 1.6 g/l/h, the concentration of the intermediate N-acetyl-D-mannosamine also increases gradually from 0 to 64 mM during the process	701881	
4.1.3.3	additional information	-	cell density of Escherichia coli BL21 (DE3) carrying the pNA1 plasmid is 37 (OD600) after fermentation for 15 h, and the highest activity for N-acetyl-D-neuraminic acid lyase is 8.472 U/ml after fermentation for 9 h, enzyme activity is assayed in reaction solution (20 mM Neu5Ac in 50 mM potassium phosphate buffer, pH 7.5)	701855	
4.1.3.3	0.015	-	N-acetyl-D-mannosamine + pyruvate, 37°C	33337	
4.1.3.3	0.0693	-	immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 10% of the free enzyme	701855	
4.1.3.3	0.14	-	crude cell extract, at 37°C	678004	
4.1.3.3	0.42	1.5	-	650651	
4.1.3.3	1.2	2.2	N-acetylneuraminic acid, 25°C	33336	
4.1.3.3	1.5	-	-	651432	
4.1.3.3	2.45	-	crude extract, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C	701855	
4.1.3.3	4.33	-	purified recombinant wild-type enzyme, pH 7.4, 30°C	727317	
4.1.3.3	6	-	purified recombinant refolded wild-type enzyme, pH 7.4, 30°C	727317	
4.1.3.3	7.65	-	pH 7.0, 37°C	713843	
4.1.3.3	10	-	-	650651	
4.1.3.3	10	-	N-acetylneuraminic acid	33330	
4.1.3.3	11	-	purified recombinant enzyme, pH 8.0, temperature not specified in the publication	726631	
4.1.3.3	11.36	-	-	651103	
4.1.3.3	12.7	-	purified recombinant His-taggged enzyme, pH 7.0, 37°C	727656	
4.1.3.3	14.6	-	after purification, at 37°C	678004	
4.1.3.3	35.5	-	N-acetylneuraminic acid	33332	
4.1.3.3	36.26	-	after 14.8fold purification, in 50 mM potassium phosphate buffer, pH 7.5, at 37°C	701855	
4.1.3.3	36.26	-	purified enzyme after expression in Escherichia coli cells, enzyme activity is assayed in reaction solution (20 mM N-acetyl-D-neuraminic acid in 50 mM potassium phosphate buffer, pH 7.5)	701855	
4.1.3.3	38.7	-	N-acetylneuraminic acid	33324	
4.1.3.3	56.8	-	N-acetylneuraminic acid	33314