2.7.7.6 additional information - - 643563, 643565, 694222 2.7.7.6 additional information - 15.4% activity in rpoS-lacZ2 translational fusion PMP77F4 (delta 50 bp), relative to pMP77FK 693588 2.7.7.6 additional information - 15.5% activity in rpoS-lacZ2 translational fusion PMP77F6 (delta 150 bp), relative to pMP77FK 693588 2.7.7.6 additional information - 23.3% activity in rpoS-lacZ2 translational fusion PMP77FdeltaEI (delta 31 bp) , relative to pMP77FK 693588 2.7.7.6 additional information - 35.8% activity in rpoS-lacZ2 translational fusion PMP77F5 (delta 100 bp), relative to pMP77FK 693588 2.7.7.6 additional information - 36.1% activity in rpoS-lacZ2 translational fusion PMP77F7 (delta 200 bp), relative to pMP77FK 693588 2.7.7.6 additional information - 55.7% activity in rpoS-lacZ2 translational fusion PMP77FdeltaE47III (delta 32 bp), relative to pMP77FK 693588 2.7.7.6 additional information - 77.7% activity in rpoS-lacZ2 translational fusion PMP77F3 (relative to pMP77FK) 693588 2.7.7.6 additional information - 78.2% activity in rpoS-lacZ2 translational fusion PMP77FINEI (insertion 78 bp), relative to pMP77FK 693588 2.7.7.6 additional information - assay for RNA polymerase activity that uses the dye RiboGreen to detect transcripts by fluorescence and is thus free of the expense, short shelf life, and high handling costs of radioisotopes. The method is relatively quick and can be performed entirely in microplate formate, allowing for the processing of dozens to hundreds of samples in parallel 660691 2.7.7.6 additional information - recombinant Xcc core RNAP lacking omega shows a 13fold decrease in enzymatic activity in comparison with that containing omega 706601 2.7.7.6 0.000178 - - 660929 2.7.7.6 0.000343 - - 643557 2.7.7.6 0.006 - - 643571 2.7.7.6 0.0156 - - 643552 2.7.7.6 0.0168 - - 643566 2.7.7.6 0.03 - - 643562 2.7.7.6 0.05 - - 643550 2.7.7.6 0.0527 - - 643558 2.7.7.6 0.0813 - - 643556 2.7.7.6 0.224 - - 643554 2.7.7.6 0.6 - - 643567 2.7.7.6 4600 - purified recombinant His6-tagged enzyme, pH 7.9, 37°C 721368