2.7.2.8 additional information - - 642351, 642355 2.7.2.8 0.0008 - pH 8.0, 70°C, activity in cell culture in growth medium with 0.2% yeast extract 725629 2.7.2.8 0.003 - pH 8.0, 70°C, activity in cell culture in minimal growth medium with 20 mM glucose and 5 mM NH4+ 725629 2.7.2.8 0.47 - purified mutant K364H enzyme, pH 8.5, 30°C 735596 2.7.2.8 0.54 - pH 7.4, 37°C 642351 2.7.2.8 0.62 - wild type enzyme, at pH 8.0 and 37°C 761643 2.7.2.8 0.77 - mutant enzyme R209A, at pH 8.0 and 37°C 761643 2.7.2.8 0.79 - mutant enzyme H268N, at pH 8.0 and 37°C 761643 2.7.2.8 1.66 - purified mutant R394K enzyme, pH 8.5, 30°C 735596 2.7.2.8 1.95 - - 672793 2.7.2.8 3.11 - purified mutant N399Q enzyme, pH 8.5, 30°C 735596 2.7.2.8 3.3 - pH 7.5, free enzyme 659433 2.7.2.8 4.97 - purified mutant S387A enzyme, pH 8.5, 30°C 735596 2.7.2.8 6.81 - purified recominant wild-type enzyme, pH 8.5, 30°C 735596 2.7.2.8 7.21 - - 642357 2.7.2.8 12 - without PII protein 673641 2.7.2.8 13.3 - pH 7.5, complex of enzyme and PII protein 659433 2.7.2.8 20.1 - pH 7.4, 37°C 720845 2.7.2.8 24 - with PII protein 673641 2.7.2.8 39.87 - purified mutant S387A enzyme, pH 8.5, 30°C 735596 2.7.2.8 44.05 - purified recominant wild-type enzyme, pH 8.5, 30°C 735596 2.7.2.8 45 - 37°C, pH 7.5 659050 2.7.2.8 130 - 37°C, pH 7.5 659050 2.7.2.8 677 - 80°C, pH 7.5 659050