2.6.1.16	additional information	-	development of an assay for glucosamine 6-phosphate synthase that measures the production of glucosamine 6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,5'-dithio-bis(2-nitrobenzoic acid), i.e. Ellman’s reagent in a discontinuous manner. Simple assay method, which can be adapted to 96-well microtiter plate format	684404	
2.6.1.16	0.001	-	mutant enzyme L468P	672444	
2.6.1.16	0.0047	-	-	639918	
2.6.1.16	0.00783	-	-	639921	
2.6.1.16	0.013	-	-	639905	
2.6.1.16	0.0144	-	-	639923	
2.6.1.16	0.021	-	glutamine 6-phosphate-synthetic activity, mutant V711F, pH 7.5, 25°C	671896	
2.6.1.16	0.05	-	glutamine 6-phosphate-synthetic activity, mutant W97G, pH 7.5, 25°C	671896	
2.6.1.16	0.095	-	amidohydrolysing activity of the mutant enzyme W97G using D-fructose 6-phosphate as substrate	671896	
2.6.1.16	0.16	-	-	639919	
2.6.1.16	0.24	-	-	639927	
2.6.1.16	0.256	-	amidohydrolysing activity of the mutant enzyme W97F using D-fructose 6-phosphate as substrate	671896	
2.6.1.16	0.38	-	mutant enzyme I271T	672444	
2.6.1.16	0.38	-	mutant enzyme I3T	672444	
2.6.1.16	0.38	-	mutant enzyme S449P	672444	
2.6.1.16	0.39	-	wild type enzyme	672444	
2.6.1.16	0.42	-	mutant enzyme A38T	672444	
2.6.1.16	0.42	-	mutant enzyme G471S	672444	
2.6.1.16	0.631	-	wild type enzyme, using D-fructose 6-phosphate as a substrate	676955	
2.6.1.16	0.866	-	-	639931	
2.6.1.16	0.89	-	amidohydrolysing activity of the wild type enzyme using D-fructose 6-phosphate as substrate	671896	
2.6.1.16	0.94	-	amidohydrolysing activity of the mutant enzyme V711F using D-fructose 6-phosphate as substrate	671896	
2.6.1.16	1.2	-	-	639911	
2.6.1.16	1.2	-	glutamine 6-phosphate-synthetic activity, mutant W97F, pH 7.5, 25°C	671896	
2.6.1.16	3.5	-	-	639913	
2.6.1.16	3.6	-	-	639911	
2.6.1.16	4	-	-	639911	
2.6.1.16	4.66	-	-	639918	
2.6.1.16	4.93	-	pH 7.0, 37°C	737920	
2.6.1.16	7.63	-	-	639922	
2.6.1.16	8	-	-	639911	
2.6.1.16	12	-	glutamine 6-phosphate-synthetic activity, wild-type, pH 7.5, 25°C	671896	
2.6.1.16	34.8	-	-	639925