2.5.1.6 additional information - - 639038, 639044, 639045, 639046, 639048, 639052, 673389, 673793, 675572, 676530 2.5.1.6 additional information - different values in refolding and purification process 639071 2.5.1.6 0.0002 - activity Peak II, adult erythrocytes 639059 2.5.1.6 0.001 - brain 705297 2.5.1.6 0.0011 - muscle 705297 2.5.1.6 0.0022 - - 639056 2.5.1.6 0.00278 - - 639046 2.5.1.6 0.0041 - pancreas 705297 2.5.1.6 0.0042 - intestine 705297 2.5.1.6 0.0061 - kidney 705297 2.5.1.6 0.039 - MAT I 639071 2.5.1.6 0.0431 - liver 705297 2.5.1.6 0.058 - tripolyphosphase activity 639078 2.5.1.6 0.065 - S-adenosylmethionine synthetase B 639048 2.5.1.6 0.068 - crude enzyme, at pH 8.5 and 45°C 737471 2.5.1.6 0.096 - glutathione/glutathione disulfide-refolded MAT III 639071 2.5.1.6 0.098 - MAT III 639071 2.5.1.6 0.1 - glutathione/glutathione disulfide-refolded MAT I 639071 2.5.1.6 0.109 - purified recombinant His-tagged isozyme SAMS1 660154 2.5.1.6 0.11 - dithiothreitol-refolded MAT III 639071 2.5.1.6 0.138 - S-adenosylmethionine synthetase A 639048 2.5.1.6 0.2 - S-adenosylmethionine synthesis activity 639078 2.5.1.6 0.28 - low-MW isozyme 639053 2.5.1.6 0.75 - - 639045 2.5.1.6 0.94 - pH 8.0, 37°C 706910 2.5.1.6 1.3 - - 658351 2.5.1.6 1.82 - pH 8, 37°C, wild-type enzyme 759131 2.5.1.6 1.828 - after 26.9fold purification, at pH 8.5 and 45°C 737471 2.5.1.6 3 - beta isoenzyme from liver 639054 2.5.1.6 3.78 - pH 8, 37°C, mutant enzyme I303V/I65V/L186V 759131 2.5.1.6 4.3 - pH 8.0, 80°C 728983 2.5.1.6 4.61 - high-MW isozyme 639053 2.5.1.6 6.02 - pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K 759131 2.5.1.6 6.42 - crude extract, at 35°C 685773 2.5.1.6 7.5 - alpha isoenzyme from liver 639054 2.5.1.6 11 - isoenzyme II 639055 2.5.1.6 12.2 - - 639052 2.5.1.6 12.4 - - 639057 2.5.1.6 18.7 - isoenzyme I 639055 2.5.1.6 23.83 - after 3.71fold purification, at 35°C 685773 2.5.1.6 32.75 - beta form from liver 639058 2.5.1.6 39 - gamma isoenzyme from liver 639054 2.5.1.6 66 - mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 78.24 - alpha form from liver 639058 2.5.1.6 108 - mutant D166N, pH 8.0 658640 2.5.1.6 108 - mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 108 - mutant D282N, pH 8.0 658640 2.5.1.6 138 - mutant D249N, pH 8.0 658640 2.5.1.6 210 - mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 216 - pH 7.0, 37°C 657534 2.5.1.6 222 - wild-type, pH 8.0 658640 2.5.1.6 240 - mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 252 - mutant K256A, pH 8.0 658640 2.5.1.6 252 - mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 318 - mutant K280A, pH 8.0 658640 2.5.1.6 330 - mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 720 - mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 792 - mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 960 - mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 3000 - wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0 658640 2.5.1.6 4400 - pH 8.2, 37°C 658771 2.5.1.6 31500 - mutant C61S, presence of glutathione 659298 2.5.1.6 32700 - mutant C35S, presence of glutathione 659298 2.5.1.6 69070 - mutant C61S, presence of dithiothreitol 659298 2.5.1.6 94470 - mutant C35S, presence of dithiothreitol 659298 2.5.1.6 95800 - wild-type, presence of glutathione 659298 2.5.1.6 110000 - wild-type, presence of dithiothreitol 659298