2.2.1.6 additional information - - 395915, 395919, 395922, 672368, 673744 2.2.1.6 additional information - assay methods 395917 2.2.1.6 additional information - effects of pyruvate and sucrose on relative ALS activity in light and dark conditions, overview 701404 2.2.1.6 additional information - isozyme substrate specificities 671290 2.2.1.6 additional information - specific activity also depends on protein concentration, with lower concentration giving lower activity, activity may be generally increased by pre-incubation at 30°C prior to assay 658566 2.2.1.6 additional information - spectrophotometric assay involving an indirect detection of the product acetolactate 395872 2.2.1.6 additional information - time lag during the production of acetolactate from pyruvate and cofactors. Time lag is temperature depended being lowest at 37°C with 250 seconds 658365 2.2.1.6 0.0023 - pH 7.5, 37°C, purified recombinant His6-tagged mutant E85A 733747 2.2.1.6 0.0043 - substrate: pyruvate, pH 7.5, 37°C, in the presence of 3.5 M KCl 727336 2.2.1.6 0.03 - mutant H351Q, 37°C, pH 7.5 657720 2.2.1.6 0.034 - pH 7.5, 37°C, purified recombinant His6-tagged mutant E85Q 733747 2.2.1.6 0.04 - mutant H351M, 37°C, pH 7.5 657720 2.2.1.6 0.04 - pH 7.5, 37°C, purified recombinant His6-tagged mutant H84A 733747 2.2.1.6 0.06 - catalytic subunit ilvI, regulatory subunit ilvN, 37°C, pH 7.6 696357 2.2.1.6 0.0616 - - 395908 2.2.1.6 0.063 - mutant DELTA567-582, 37°C, pH 7.4 657852 2.2.1.6 0.063 - pH 7.5, 37°C, purified recombinant His6-tagged mutant E85D 733747 2.2.1.6 0.068 - mutant DELTA630, 37°C, pH 7.4 657852 2.2.1.6 0.09 - mutant D374A/D375A, pH 7.5, 37°C 658223 2.2.1.6 0.135 - - 395914 2.2.1.6 0.15 - purified enzyme, at pH 7.4 and 37°C 758259 2.2.1.6 0.17 - isolated catalytic subunit, pH 7.5, 37°C 719375 2.2.1.6 0.19 - pH 7.5, 37°C, purified recombinant His6-tagged mutant H84T 733747 2.2.1.6 0.22 - pH 7.5, 37°C, purified recombinant His6-tagged mutant Q86A 733747 2.2.1.6 0.4075 - - 395896 2.2.1.6 0.64 - mutant D374E/D375E, pH 7.5, 37°C 658223 2.2.1.6 0.65 - mutant D375A, pH 7.5, 37°C 658223 2.2.1.6 0.66 - - 395877 2.2.1.6 0.77 - mutant D374E, pH 7.5, 37°C 658223 2.2.1.6 0.844 - - 395918 2.2.1.6 0.91 - mutant D374A, pH 7.5, 37°C 658223 2.2.1.6 1.02 - mutant K255F, pH 7.5, 37°C 657724 2.2.1.6 1.13 - holoenzyme, pH 7.5, 37°C 719375 2.2.1.6 1.13 - pH 7.5, 37°C 719375 2.2.1.6 1.3 - catalytic subunit ilvB, regulatory subunit ilvB, 37°C, pH 7.6 696357 2.2.1.6 1.5 - calculated per subunit, purified recombinant His-tagged enzyme 697061 2.2.1.6 1.5 - pH 7.6, 37°C 719117 2.2.1.6 1.5 - purified recombinant enzyme 695852 2.2.1.6 1.53 - mutant D375E, pH 7.5, 37°C 658223 2.2.1.6 1.55 - wild-type, pH 7.5, 37°C 658223 2.2.1.6 1.58 - mutantH351F, 37°C, pH 7.5 657720 2.2.1.6 1.59 - - 395910 2.2.1.6 1.6 - mutant E47A, 37°C, pH 7.6 657912 2.2.1.6 1.73 - wild-type, 37°C, pH 7.4 657852 2.2.1.6 1.87 - wild-type, pH 7.5, 37°C 657724 2.2.1.6 2 - mutant E47Q, 37°C, pH 7.6 657912 2.2.1.6 2.17 - mutant K255Q, pH 7.5, 37°C 657724 2.2.1.6 2.6 - mutant M250A with His-tag, pH 7.6, 37°C 660390 2.2.1.6 2.67 - - 395916 2.2.1.6 2.8 - mutant R276K with His-tag, pH 7.6, 37°C 660390 2.2.1.6 2.8 - pH 7.5, 37°C, purified recombinant His6-tagged wild-type enzyme 733747 2.2.1.6 2.8 - purified catalytic subunit 673626 2.2.1.6 3.12 - large subunit, pH 7.9, 55°C 736351 2.2.1.6 3.23 - mutant H392M, 37°C, pH 7.5 657720 2.2.1.6 3.4 - mutant F109M, pH 7.6, 37°C 718875 2.2.1.6 4.15 - mutant K299Q pH 7.5, 37°C 657724 2.2.1.6 4.4 - mutant enzyme W574L 395903 2.2.1.6 4.6 - purified holoenzyme 673626 2.2.1.6 5.4 - crude extract, at pH 7.0 and 80°C 733241 2.2.1.6 5.78 - wild-type, 37°C, pH 7.5 657720 2.2.1.6 6.25 - - 395912 2.2.1.6 6.8 - catalytic subunit, pH 7.0, 30°C 657909 2.2.1.6 7.88 - - 395902 2.2.1.6 7.96 - mutant Y35N/K139R/V172A/H474R, pH 7.0, 60°C 735039 2.2.1.6 8 - pH 7.5, 37°C 720392 2.2.1.6 8.13 - mutant H747R, pH 7.0, 60°C 735039 2.2.1.6 8.2 - mutant V375A, pH 7.6, 37°C 718875 2.2.1.6 8.28 - - 395889 2.2.1.6 8.51 - mutant S653N 395903 2.2.1.6 9.16 - holoenzyme, pH 7.9, 55°C 736351 2.2.1.6 9.3 - catalytic subunit ilvI, regulatory subunit ilvM, 37°C, pH 7.6 696357 2.2.1.6 9.4 - wild-type AHAS III, pH 7.6, 37°C 696357 2.2.1.6 11 - mutant W574 395903 2.2.1.6 11 - presence of 20 mM acetate, pH 7.5, 37°C 720392 2.2.1.6 11.9 - mutant W464L with His-tag, pH 7.6, 37°C 660390 2.2.1.6 12 - mutant enzyme A122V 395903 2.2.1.6 12.6 - wild-type, pH 7.0, 60°C 735039 2.2.1.6 13.5 - pH 7.5, 37°C 719329 2.2.1.6 17 - wild-type with His-tag, pH 7.6, 37°C 660390 2.2.1.6 20 - wild-type, 37°C, pH 7.6 657912 2.2.1.6 20 - wild-type, pH 7.6, 37°C 660390 2.2.1.6 20.9 - wild-type AHAS III, truncated regulatory subunit ilvH-DELTA 86, pH 7.6, 37°C 696357 2.2.1.6 22.7 - mutant V375I, pH 7.6, 37°C 718875 2.2.1.6 25.3 - - 395879 2.2.1.6 25.6 - - 395892 2.2.1.6 32 - catalytic subunit ilvB, truncated regulatory subunit ilvB-DELTA86, 37°C, pH 7.6 696357 2.2.1.6 34.3 - wild-type, pH 7.6, 37°C 718875 2.2.1.6 35.4 - at pH 6.5 and 37°C 756209 2.2.1.6 36 - presence of 20 mM acetate, pH 7.5, 37°C 720392 2.2.1.6 42 - pH 7.5, 37°C 720392 2.2.1.6 49 - - 395881 2.2.1.6 49 - catalytic subunit plus small subunit, pH 7.0, 30°C 657909 2.2.1.6 49.8 - catalytic subunit ilvB, regulatory subunit ilvM, 37°C, pH 7.6 696357 2.2.1.6 50.8 - wild-type AHAS I, 37°C, pH 7.6 696357 2.2.1.6 53 - fusion protein with N-terminal oligohistidine at the large subunit 395904 2.2.1.6 60.4 - wild-type AHAS II, 37°C, pH 7.6 696357 2.2.1.6 60.5 - reconstituted, recombinant holoenzyme 672368 2.2.1.6 63.6 - recombinant holoenzyme 672368 2.2.1.6 150 - 30°C, 60 ng protein per assay 658566 2.2.1.6 163 - after 30.2fold purification, at pH 7.0 and 80°C 733241 2.2.1.6 163 - pH 7.0, 85°C, purified recombinant catalytic subunit 733241 2.2.1.6 200 - 37°C 660559 2.2.1.6 267 - 30°C, 90 ng protein per assay 658566 2.2.1.6 2883 - pH 7.0, 30°C, sulfonylurea-susceptible biotype 660058 2.2.1.6 2950 - pH 7.0, 30°C, sulfonylurea-resistant biotype 660058