1.14.13.29 0.000037 - microsomal fraction 438907 1.14.13.29 0.000494 - - 438904 1.14.13.29 0.00275 - malaria infected mouse, real time kinetic method 697776 1.14.13.29 0.00411 - mouse without infection, real time kinetic method 697776 1.14.13.29 0.0128 - substrate 2-chloro-4-nitrophenol, pH 7.6, 22°C 763974 1.14.13.29 0.0179 - substrate 4-nitrocatechol, pH 7.6, 22°C 763974 1.14.13.29 0.034 - substrate 4-nitrophenol, pH 7.6, 22°C 763974 1.14.13.29 11.2 - cell extract, 0.3 mM 4-nitrophenol as substrate, 1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer (pH 7.5), 22°C 698597 1.14.13.29 12.8 - substrate 2-chloro-4-nitrophenol, pH 7.6, 23°C 763974 1.14.13.29 17.9 - substrate 4-nitrocatechol, pH 7.6, 23°C 763974 1.14.13.29 24.5 - HiTrap Q-Sepharose purified enzyme, 0.3 mM 4-nitrophenol as substrate, 1 mM NADH, 5 M FAD, 1 mM DTT, NphA1 (approximately 0.5 mg of purified protein), and NphA2 (approximately 0.1 mg of purified protein) in 1 ml of 50 mM Tris-HCl buffer (pH 7.5), 22°C 698597 1.14.13.29 34.2 - substrate 4-nitrophenol, pH 7.6, 23°C 763974 1.14.13.29 98.3 - entire male pig, pH 6.8 695586 1.14.13.29 132.8 - surgically castrated pig, pH 6.8 695586 1.14.13.29 161.5 - immunocastrated pig, pH 6.8 695586