1.14.13.22 additional information - - 438991 1.14.13.22 additional information - activity below 0.1 micromol/min/mg protein, substrate cyclohexanone, induced (0.1 mM isopropylthiogalactoside) cell culture extracts, BHI medium or modified Riesenberg medium, 30°C 699080 1.14.13.22 additional information - activity is dependent on substrate concentration and buffer conditions 438989 1.14.13.22 additional information - whole cell activity to produce epsilon-caprolactone from cyclohexanone: initially 72 micromol/min/g dry cells, linearly falling to 14-18 micromol/min/g dry cells, sodium-potassium phosphate buffer, pH 7.0 with glucose (transferred from modified Riesenberg medium used for cultivation), 30°C 699080 1.14.13.22 0.4 - substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, modified Riesenberg medium, 30°C 699080 1.14.13.22 0.5 - substrate cyclohexanone, induced (1.0 mM isopropylthiogalactoside) cell culture extracts, BHI medium, 30°C 699080 1.14.13.22 0.85 - mutant W492A, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C 698499 1.14.13.22 1.4 - H163Q mutant enzyme 658433 1.14.13.22 2.1 - - 438968, 438970, 438978 1.14.13.22 3.57 - with cyclohexanone as substrate, pH 7.5 657585 1.14.13.22 3.68 - with cyclohexanone as substrate, pH 7.5 657585 1.14.13.22 3.75 - with cyclohexanone as substrate, pH 7.5 657585 1.14.13.22 5.4 - H96Q mutant enzyme 658433 1.14.13.22 6 - wild type enzyme, cyclohexanone, 50 mM Tris-HCl buffer (pH 9.0), 0.1 mM NADPH, 25°C 698499 1.14.13.22 6.8 - substrate cyclohexanone, pH 8.0, 25°C 724071 1.14.13.22 8 10 partially purified enzyme, substrate 4-methylcyclohexanone 438973 1.14.13.22 9.1 - purified recombinant enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one 675461 1.14.13.22 9.2 - purified native enzyme, substrate bicyclo[3.2.0]hept-2-en-6-one 675461 1.14.13.22 9.9 - purified enzyme 438984 1.14.13.22 14 - purified recombinant His-tagged enzyme expressed in yeast 438992 1.14.13.22 15 - purified native enzyme 438992 1.14.13.22 15.9 - wild type enzyme 658433 1.14.13.22 19.9 - purified recombinant His-tagged enzyme expressed in E. coli 438992 1.14.13.22 21 - purified enzyme 438984 1.14.13.22 24.75 - purified His-tagged recombinant enzyme 685723 1.14.13.22 678 - purified enzyme 438993