3.4.22.14 fruit juice 81474 3.4.22.14 sarcocarp - 81476 3.4.22.14 fruit - 650650, 669155, 684521, 686853, 708861, 708946, 709104, 709327, 717622, 718236, 81481, 81484, 81485, 81486, 81488, 81494 3.4.22.14 fruit actinidin mRNAs for acidic and basic proteins are expressed at comparable levels throughout ripening. Actinidin mRNA expression is highest in fruit at harvest, expression decreases as fruit ripened and is much lower in the core compared with outer pericarp tissue. Low levels of a basic actinidin protein (KFAct2a) in ripe Actinidia chinensis fruit. Extremely high levels of an acidic actinidin protein (KFAct1a) is detected in Actinidia chinensis EM4 fruit. This acidic protein appears to be absent in Actinidia chinensis Hort16A 686912 3.4.22.14 fruit actinidin mRNAs for acidic and basic proteins are expressed at comparable levels throughout ripening. Actinidin mRNA expression is highest in fruit at harvest, expression decreases as fruit ripened and is much lower in the core compared with outer pericarp tissue. Low levels of a basic actinidin protein (KFAct2a) in ripe Actinidia deliciosa fruit. Extremely high levels of an acidic actinidin protein (KFAct1a) is detected in Actinidia deliciosa fruit. Both the basic and acidic actinidin isoforms in Actinidia deliciosa are active cysteine proteases 686912 3.4.22.14 pericarp actinidin is present in small cells, but not large cells in the outer pericarp of mature Actinidia deliciosa fruit at harvest. Within the small cells, actinidin is localised diffusely in the vacuole, associated with the plasma membrane, and in a layer in the plastids near starch granules 686912 3.4.22.14 juice - 689327 3.4.22.14 fruit compared to Act d 8 and Act c 8, Act d 1 transcript is stronger expressed as determined by RT-PCR 700289 3.4.22.14 pulp - 718203, 752807