3.1.1.79 3T3-L1 cell - 714794 3.1.1.79 abdomen highest mRNA levels of isoforms HslB1 and HslB2 are both observed in abdominal fat 750567 3.1.1.79 adipocyte - -, 665562, 665964, 677942, 714034, 714319, 714794 3.1.1.79 adipocyte adipocytes retrovirally engineered from murine embryonic fibroblasts 680650 3.1.1.79 adipocyte dydrogesterone and norethisterone regulate expression of hormone-sensitive lipase in human subcutaneous abdominal adipocytes 679453 3.1.1.79 adipocyte women with polycystic ovary syndrome or obesity have significantly decreased expression of hormone-sensitive lipase. Knock down of hormone-sensitive lipase by RNAi reduces basal and catecholamine-induced lipolysis. Treatment of mesenchymal stem cells with a selective inhibitor of hormone-sensitive lipase during and/or after differentiation in adipocytes reduces basal lipolysis by 50% 677465 3.1.1.79 adipose tissue - -, 133829, 646087, 654152, 654401, 654572, 654765, 654792, 655276, 655309, 655415, 655960, 656393, 656598, 690342, 692007, 703845 3.1.1.79 adipose tissue perirenal 654901 3.1.1.79 adrenal gland - 654401, 655309, 665562, 703845 3.1.1.79 beta cell - 692007 3.1.1.79 blood - 692007 3.1.1.79 brown adipose tissue - 654162, 655415 3.1.1.79 C8PA cell HEK-293 fibroblasts stably expressing fatty acid transport protein 1 680839 3.1.1.79 cardiac muscle - 654471 3.1.1.79 germ cell postmeiotic germ cells express a specific HSL isoform that includes a 313 amino acid N-terminus encoded by a testis-specific exon T1 729643 3.1.1.79 granulosa cell - 693448 3.1.1.79 heart - -, 654401, 690342, 714033 3.1.1.79 hepatocyte - 693515 3.1.1.79 hepatocyte incubation of hepatocytes with insulin in vitro reduces the mRNA levels of isoform HslB2 rather than HslB1 750567 3.1.1.79 HT-29 cell - 654402 3.1.1.79 kidney - 749831 3.1.1.79 left ventricle - 714033 3.1.1.79 Leydig cell - -, 730033 3.1.1.79 liver - 693515 3.1.1.79 liver the expression of isoform HslB2 is significantly higher than that of HslB1 in liver, red muscle, and white muscle 750567 3.1.1.79 luteal cell shows HSL staining in cytoplasm during proestrus and estrus, in the nucleus during metestrus, and in cytoplasm and the nucleus during diestrus 693448 3.1.1.79 MA-10 cell MA-10 mouse Leydig tumor cells -, 730033 3.1.1.79 macrophage - 646087, 654152, 655309, 730333 3.1.1.79 mammary epithelial cell bMEC 690757 3.1.1.79 mammary epithelium - 654793 3.1.1.79 mammary gland - 690757 3.1.1.79 mammary gland in virgin rats, immunoreactive hormone-sensitive lipase is observed in mammary adipocytes, diffuse staining is detected in epithelial cells. Positive staining for hormone-sensitive lipase is detected in the two types of cells in pregnant and lactating animals. As pregnancy advances, the staining intensity of immunoreactive hormone-sensitive lipase increases in the epithelial cells parallel to their proliferation, attaining the maximum during lactation. Both hormone-sensitive lipase protein and activity are lower in mammary glands from 20 and 21 day pregnant rats than from those of virgin rats. They return to virgin values on days 1 and 4 of lactation 665964 3.1.1.79 mature ovarian follicle - 693448 3.1.1.79 milk - 665964 3.1.1.79 additional information granulosa cells and oocytes of primordial follicles are immunonegative 693448 3.1.1.79 additional information HSL expression in theca cells and oocytes decrease during follicular atresia 693448 3.1.1.79 muscle activity is similar in untrained and trained skeletal muscles both before and after prolonged exercise 679605 3.1.1.79 muscle the expression of isoform HslB2 is significantly higher than that of HslB1 in liver, red muscle, and white muscle 750567 3.1.1.79 oocyte - 693448 3.1.1.79 ovarian epithelium - 693448 3.1.1.79 ovary - 654401, 665562, 693448 3.1.1.79 oviduct - 693448 3.1.1.79 oviductal ampulla - 693448 3.1.1.79 pancreas - 690762 3.1.1.79 pancreatic beta cell - 665562 3.1.1.79 pancreatic beta cell located in close association to insulin granules in beta-cells 690762 3.1.1.79 pancreatic beta cell the beta-cell isoform of HSL is involved in maintaining lipid homeostasis in islets and contributes to the proper control of glucose stimulated insulin secretion 679455 3.1.1.79 parenchymal cell - 693515 3.1.1.79 peritoneal macrophage MPM 690756 3.1.1.79 placenta cotyledon 646084 3.1.1.79 RAW-264.7 cell - 690756 3.1.1.79 skeletal muscle - 654155, 654401, 654475, 666157, 690345, 691998, 751397 3.1.1.79 skeletal muscle enzyme activity increases early in exercise and remains elevated for the duration of the exercise 665344 3.1.1.79 skeletal muscle expressed at a higher level in oxidative fibres than in glycolytic fibres. The two physiological stimuli adrenaline and contraction increase enzyme activity in muscle, and the effects are partially additive. Adrenaline acts via beta-adrenergic activation of cAMP-dependent protein kinase, while the effect of contractions is mediated by protein kinase C, at least partly via the extracellular signal-regulated kinase pathway. Endurance training diminishes the sensitivity of muscle hormone-sensitive lipase to adrenaline but increases the contraction-mediated activation 666773 3.1.1.79 skeletal muscle in the skeletal muscle at rest there is a high constitutive level of hormone-sensitive lipase activity, which is not a function of biopsy freezing. The combination of low adrenaline and Ca2+ levels and resting levels of insulin appears to dictate the levels of activity of hormone-sensitive lipase at rest. During the onset of low and moderate aerobic exercise enzyme activity is activated by contractions, in the apparent absence of increases in circulating adrenaline. However, adrenaline may contribute to the early activation of the enzyme during intense aerobic exercise. The contraction-induced activation appears to be related to the increase in protein kinase C and extracellular signal regulated kinase activity associated with Ca2+ and/or other unknown activators. As low- and moderate-intensity exercise continues beyond a few min, activation by adrenaline through the cAMP cascade and protein kinase A also appears to occur. With prolonged moderate-intensity exercise beyond 1–2 h and sustained high-intensity exercise, HSL activity decreases despite continuing increases in adrenaline, possibly as a result of increasing accumulation of free AMP, activation of AMP-activated kinase and phosphorylation of inhibitory sites on the hormone-sensitive lipase. Intramuscular factors dominate the control of hormone-sensitive lipase activity, with hormonal factors playing a smaller role 666774 3.1.1.79 skeletal muscle muscle hormone-sensitive lipase mRNA (80%) and protein content (50%) are higher in women than in men. Total activity increases during exercise (47%) but does not differ between sexes. Accordingly, hormone-sensitive lipase specific activity (HSL activity per HSL protein content) increased during exercise (62%) and is higher in men than in women 677463 3.1.1.79 small intestine HSL accounts for the entire neutral cholesteryl ester hydrolase activity of enterocytes 655309 3.1.1.79 testis - -, 654418, 655309, 655415, 703845, 729643, 730033 3.1.1.79 theca cell - 693448 3.1.1.79 THP-1 cell - 730333 3.1.1.79 uterus HSL is found in the epithelial cells nuclei 693448 3.1.1.79 white adipose tissue - -, 665964, 677470, 714340, 714840, 730002 3.1.1.79 white adipose tissue adipose triglyceride lipase and hormone-sensitive lipase are responsible for more than 95% of the triglyceride hydrolase activity present in murine white adipose tissue 680733