2.8.2.2 adipose tissue male white epididymal fat pad, no expression in brown fat 692086 2.8.2.2 adrenal gland - 645780, 645781, 645782, 645783, 645785, 645797, 760542 2.8.2.2 adrenal gland adult and fetal adrenal 761169 2.8.2.2 adrenal gland fetal, low activity, isoform SULT1A1 662862 2.8.2.2 atheroma cell - 675275 2.8.2.2 BEL-7402 cell - 726332 2.8.2.2 brain - 692098, 760542 2.8.2.2 brain enzyme localization in neural fibers, posterior region, and dorsal part of the brain, overview 690709 2.8.2.2 brain fetal 761169 2.8.2.2 brain fetal brain 738296 2.8.2.2 brain fetal, high activity in choroid plexus, isoform SULT1A1 662862 2.8.2.2 brain higher expression level of SULT2B1 675680 2.8.2.2 brain isoform SULT2B1a 661823 2.8.2.2 brain SULT2B1a is only expressed in brain and testis 661823 2.8.2.2 brain very low level 760542 2.8.2.2 breast - 675680 2.8.2.2 breast cancer tissue 645741 2.8.2.2 bronchus - 675680 2.8.2.2 Caco-2 cell - 738482 2.8.2.2 colon - 738296, 761169 2.8.2.2 colon adult, isoform SULT1A1 662862 2.8.2.2 colon adult, isoform SULT1A3 662862 2.8.2.2 DU-145 cell - 761662 2.8.2.2 embryo - 645782 2.8.2.2 epidermis epidermal keratinocyte 645792 2.8.2.2 epididymis - 645775, 645777, 645794 2.8.2.2 epididymis SULT2A1 expression is virtually confined to the body in the epididymis. The enzyme is secreted into the ductal lumen via an apocrine mechanism. In addition to the ductal epithelium, immunostaining is observed in spherical particles situated in the ductal lumen and in vascular endothelial cells of the epididymal connective tissue 761704 2.8.2.2 epididymis SULT2B1 expression is increased significantly along the epididymis, coexpression with HSD3B1. The enzyme is secreted into the ductal lumen via an apocrine mechanism. Immunolocalization study of SULT2B1. SULT2B1-mRNA expression is highest in the distal part of the epididymal tail in muscular cells 761704 2.8.2.2 epithelium - 645783, 645792, 645797, 761704 2.8.2.2 fetus - 645782, 645783, 645790, 645797 2.8.2.2 gastric epithelium abundant SULT2B1 expression in normal gastric epithelium 761782 2.8.2.2 heart - 760542 2.8.2.2 heart low level 760542 2.8.2.2 HEP-3B cell - 726332 2.8.2.2 Hep-G2 cell - 677218, 738482, 761169, 761727, 761897 2.8.2.2 Hepa1-6 cell - 739057 2.8.2.2 hepatic oval cell - -, 737574, 760454 2.8.2.2 hepatocellular carcinoma cell - 726332 2.8.2.2 hepatocyte - -, 645741, 645782, 645783, 645790, 760454, 761897 2.8.2.2 hepatocyte primary culture 693816 2.8.2.2 hepatoma cell - 761727 2.8.2.2 hepatoma cell SULT2B1b mRNA levels in clinical hepatocarcinoma tumor samples are higher than in the non-tumorous tissue adjacent to the tumors -, 760454 2.8.2.2 Huh-7 cell - 726332 2.8.2.2 Huh-7 cell hepatocellular carcinoma cell line 645741 2.8.2.2 ileum - 761704 2.8.2.2 intestine - 737572, 760965 2.8.2.2 intestine fetal intestinal villi 645783 2.8.2.2 intestine fetal, high activity, isoform SULT1A3 662862 2.8.2.2 intestine isoform SULT2B1b 661823 2.8.2.2 intestine lower expression level of SULT2B1 675680 2.8.2.2 intestine main expression of SULT2B1b 661823 2.8.2.2 jejunum high level 760542 2.8.2.2 jejunum moderate level 760542 2.8.2.2 jejunum predominantly 760542 2.8.2.2 jejunum very low level 760542 2.8.2.2 kidney - 645797, 674044, 760542 2.8.2.2 kidney fetal, low activity, isoform SULT1A1 662862 2.8.2.2 kidney high level 760542 2.8.2.2 kidney isoform SULT2B1b 661823 2.8.2.2 kidney main expression of SULT2B1b 661823 2.8.2.2 kidney predominantly 760542 2.8.2.2 kidney present in proximal and distal tubules, loops of Henle and collecting ducts, but at no time expressed in the vascular glomerulus 645782 2.8.2.2 larva liver 656447 2.8.2.2 Leydig cell - 761704 2.8.2.2 liver - -, 645741, 645773, 645774, 645776, 645778, 645779, 645780, 645782, 645783, 645784, 645785, 645786, 645789, 645790, 645793, 645797, 660699, 674044, 692086, 692098, 693816, 703834, 737572, 738101, 738296, 738909, 739057, 760454, 760542, 760965, 761169, 761704, 761727, 761897 2.8.2.2 liver adult and and fetal liver 761169 2.8.2.2 liver adult and fetal, isoform SULT1A1 662862 2.8.2.2 liver expression of three of the SULT2A isoforms is abundant in pre-pubertal male and female mouse liver but is essentially silenced in the livers of adult male mice, overview 690852 2.8.2.2 liver larval 656447 2.8.2.2 liver predominantly 760542 2.8.2.2 liver predominately 760542 2.8.2.2 LNCaP cell - 675680, 761662, 761881 2.8.2.2 LNCaP-C4-2 cell - 761662, 761881 2.8.2.2 lung - 675680, 760542 2.8.2.2 MCF-7 cell - 675275, 675604, 675680 2.8.2.2 additional information androgen responsive cell lines LNCaP, VCaP, RWPE-1, and the castration nonresponsive line C4-2 generally express higher levels of SULT2B1b than AR- cell lines, such as PC-3 and DU-145 cells 761662 2.8.2.2 additional information no estrogen sulfotransferase expression in brown adipose tissue, differential expression of estrogen sulfotransferase in fat and liver, overview 692086 2.8.2.2 additional information no expression in 293T cells 675275 2.8.2.2 additional information no expression of SULT2B1b in alveolar tissue and in liver cytosol 675680 2.8.2.2 additional information no expression of SULT2B1b in placental, prostate and breast tissues in rodents 675680 2.8.2.2 additional information quantitative real-time RT-PCR enzyme expression analysis in female and male tissue samples, tissue distribution and expression pattern, overview 760542 2.8.2.2 additional information quantitative real-time RT-PCR enzyme expression analysis in female and male tissue samples, tissue distribution and expression pattern, overview. SULT1A3 is expressed in all the tissues, but most predominantly in jejunum, followed by liver 760542 2.8.2.2 additional information SULT2B1 expression is absent or low in the testis. Tissue samples were collected from a total number of ten postpubertal crossbreed boars (nine German Landrace × Pietrain, one Large White) aged between 10 and 16 months. When measuring P5-ST activities in cytosols, conversion rates of the substrate is only marginal in testis, epididymal head and the initial segment of the epididymal body. They increased in the subsequent segments of the epididymal body and are maximal in the epididymal tail. When measuring the sulfonation of pregnenolone (P5) in homogenates prepared from the testis and selected segments (EH1, ET1) of the epididymis, as in cytosols sulfonation activities are high in epididymal tail and only marginal in epididymal head 761704 2.8.2.2 additional information the SULT2B1 isoforms SULT2B1a and SULT2B1b are expressed in different tissues. SULT1B1a is expressed in colon, ovary, and fetal brain, and SULT2B1b in liver, colon, small intestine, placenta, ovary, uterus, and prostate 761169 2.8.2.2 additional information tissue distribution of isozymes SULT2B1a and SULT2B1b expression, overview 661823 2.8.2.2 additional information tissue samples were collected from a total number of ten postpubertal crossbreed boars (nine German Landrace x Pietrain, one Large White) aged between 10 and 16 months. Immunolocalization study of SULT2A1. In epididymal samples, DHEA-ST activities are low in the head and in the proximal part of the body. In the distal body and tail, activities are in a similar range as observed in testicular samples, again with a considerable variability between individual animals and also between different segments of the organ 761704 2.8.2.2 neuron - 692098 2.8.2.2 ovary - 738296, 760542, 761169 2.8.2.2 PC-3 cell - 761662 2.8.2.2 placenta - 645741, 645782, 645785, 645794, 645797, 675680, 738296, 761169 2.8.2.2 primary cell - 761782, 761897 2.8.2.2 prostate - 694547 2.8.2.2 prostate adenocarcinoma cell - 675680 2.8.2.2 prostate gland - 738296, 761169, 761662, 761881 2.8.2.2 prostate gland prostate epithelial cells, prostate benign prostatic hyperplasia, prostate adenocarcinoma and LNCaP prostate adenocarcinoma cells 675680 2.8.2.2 prostate gland cancer cell - 761662, 761881 2.8.2.2 root - 739274 2.8.2.2 RWPE-1 cell - 761662 2.8.2.2 sarcoma cell endometrial stromal, from 29 patients, reduced or no expression of EST 694547 2.8.2.2 seedling - 739274 2.8.2.2 shoot - 739274 2.8.2.2 skin - 645792, 645797 2.8.2.2 skin isoform SULT2B1b 661823 2.8.2.2 skin main expression of SULT2B1b 661823 2.8.2.2 skin SULT2B1b, but not SULT2B1a 675680 2.8.2.2 small intestine - 738296, 760542, 761169 2.8.2.2 SMMC-7721 cell - 726332 2.8.2.2 spinal cord higher expression level of SULT2B1 675680 2.8.2.2 stomach - 761782 2.8.2.2 stomach epithelium - 761782 2.8.2.2 T-47D cell - 675680 2.8.2.2 TE-671 cell medulloblastoma-derived cells 692098 2.8.2.2 testis - 645797, 760542, 761704 2.8.2.2 testis interstitial cells of the testis 645783 2.8.2.2 testis isoform SULT2B1a 661823 2.8.2.2 testis low level 760542 2.8.2.2 testis moderate to low level 760542 2.8.2.2 testis SULT2B1a is only expressed in brain and testis 661823 2.8.2.2 testis testis-specific isoform 645794 2.8.2.2 uterine endometrium - 694547, 738296 2.8.2.2 uterus - 645794, 645797, 738296, 760542, 761169 2.8.2.2 VCaP cell - 761662 2.8.2.2 WB-F344 cell - 737574 2.8.2.2 white adipose tissue of male, but not of female mice. Testosterone-dependent, gender-specific expression of estrogen sulfotransferase in adipose tissue 692086