2.4.1.123 cotyledon highest activity among all of the components of seed, much poorer source than Cucurbita pepo mature leaves 638602 2.4.1.123 endosperm LeGOLS-1 mRNA is present in endosperm caps 638603 2.4.1.123 flower mature and senescent 759361 2.4.1.123 fruit capsule dry capsules 759361 2.4.1.123 hypocotyl BnGolS1 members 759361 2.4.1.123 leaf - 661355, 663131, 688075, 706156, 706178, 706379, 736950 2.4.1.123 leaf 7th node from the growing tip, from fruiting plants 638601 2.4.1.123 leaf in 15 DAG seedlings, mature full-length OsGolS1 is expressed only in leaf sheath of etiolated plants. In contrast, the q-PCR indicative of pre-mRNA accumulation relative to actin (housekeeping gene) expression shows a strikingly different profile. The pre-mRNA of OsGolS1 is accumulated in the leaf tissue of etiolated plants at a high level, about fourfold to the accumulation of actin. In one-month-old plants, mature OsGolS1 is found only in leaf sheath 760001 2.4.1.123 leaf in leaves of Arabidopsis thaliana plants overexpressing heat shock transcription factor A2, the transcription of GolS1, -2, and -4 and raffinose synthase 2 (RS2) is highly induced. In leaves of the wild-type plants, treatment with 50 mM methylviologen increases the transcript levels of GolS1, -2, -3, -4, and -8 and the total activities of GolS isoenzymes 689615 2.4.1.123 leaf in one-month-old plants mature OsGolS1 is found only in leaf sheath, OsGolS2 shows both mature and pre-mRNA in sheath. OsGolS2 pre-mRNA is expressed 25fold in leaf sheath 760001 2.4.1.123 leaf mature leaves, much richer source than Phaseolus vulgaris cotyledons 638602 2.4.1.123 leaf mature, high expression of BnGolS3 members 759361 2.4.1.123 leaf source and sink leaves, GolS-1 is source leaf-specific, equally weak expression of GolS-2 in all leaf types 638605 2.4.1.123 mesophyll - 706178 2.4.1.123 mesophyll GolS-1 is primarily expressed in the mesophyll, the site of raffinose family oligosaccharides storage 638605 2.4.1.123 additional information enzyme activity of GS correlates with GolS transcript accumulation and galactinol accumulation 759987 2.4.1.123 additional information enzyme expression is heat-inducible being induced by heat-shock proteins in all vegetative tissues 663131 2.4.1.123 additional information gene MfGolS1 transcript is not detected in untreated vegetative tissues, but greatly induced in leaves and slightly in lateral roots, but not in axial roots, stem and petioles, after cold treatment 736950 2.4.1.123 additional information plant-wide developmental and tissue-specific profile of isozymes OsGolS1 and OsGolS2 functional expression, overview. In 3-month-old plants, mature OsGolS1 is found in late panicle, shoot, and also in root tissue with a precursor 760001 2.4.1.123 additional information plant-wide developmental and tissue-specific profile of isozymes OsGolS1 and OsGolS2 functional expression, overview. OsGolS2 pre-mRNA is expressed in all light-grown tissue, ranging from 25fold in leaf sheath to twofold in the root compared to actin. The etiolated plants also show high accumulation of pre-mRNA in roots. In one-month-old plants mature OsGolS1 is found only in leaf sheath, OsGolS2 shows both mature and pre-mRNA in sheath. OsGolS2 is not found in 3-month-old plants 760001 2.4.1.123 additional information tissue-specific and hormone-induced expression patterns of BnGolS. BnGolS6 and BnGolS7 members are not expressed in most tissues and were only weakly expressed in specific tissues during a specific development stage 759361 2.4.1.123 additional information tissue-specific and hormone-induced expression patterns of NtGolS. NtGolS2-2, NtGolS2-3 and all of the NtGolS1 members display constitutive expression in all tissues tested. NtGolS1-1 and NtGolS1-2 are highly expressed in stems and dry capsules, while NtGolS1-3 is highly expressed in mature flowers and senescent flowers. NtGolS2-2, NtGolS2-3, NtGolS2-4 and NtGolS2-6 display high expression in dry capsules, while NtGolS2-1 and NtGolS2-5 have no detectable expression 759361 2.4.1.123 panicle - 760001 2.4.1.123 pericarp strong expression of BnGolS2 and BnGolS3 members 40 days after flowering 759361 2.4.1.123 phloem GolS-2 is primarily expressed in the phloem-associated intermediary cells known for their role in raffinose family oligosaccharides phloem loading 638605 2.4.1.123 radicle LeGOLS-1 mRNA is most abundant in radicle tips 638603 2.4.1.123 root - 760001 2.4.1.123 root BnGolS4-1 759361 2.4.1.123 root lateral 736950 2.4.1.123 seed - 638603, 662539, 736959 2.4.1.123 seed during lentil seed development, isozyme LcGolS1 transcripts show higher accumulation during 26-32 days after flowering (DAF) corresponding to seed desiccation, while isozyme LcGolS2 shows maximum accumulation at 24 DAF, prior to increase in LcGolS1 transcripts 759987 2.4.1.123 seed high expression of BnGolS1-1 and BnGolS1-3 40 days after flowering, BnGolS6-4 is weakly expressed 10 days after flowering, and BnGolS7-2 is weakly expressed 30 and 40 days after flowering 759361 2.4.1.123 seed high level of AtGolS1 and 2 expression in mature seeds, but very low of AtGolS3 638604 2.4.1.123 seedling - 706379, 759361, 760001 2.4.1.123 shoot - 760001 2.4.1.123 silique strong expression of BnGolS2 and BnGolS3 members 40 days after flowering 759361 2.4.1.123 spikelet - 760001 2.4.1.123 stem - 759361