1.1.1.188 3T3-L1 cell AKR1B3 mRNA 9 is expressed in preadipocytes, and its level increases about 4fold at day 1 after initiation of adipocyte differentiation, and then quickly decreases the following day to a level lower than that in the preadipocytes 712494 1.1.1.188 adrenal gland - 389464, 687797 1.1.1.188 adrenal gland abundant expression 760437 1.1.1.188 amastigote - -, 761141 1.1.1.188 amnion expression of genes for PGE2 synthesis, with low levels of prostaglandin transporters and dehydrogenase 713047 1.1.1.188 blood vessel endothel, isozymes PGF synthase I and II 655139 1.1.1.188 brain - 654851, 661605 1.1.1.188 brain enzyme is expressed preferentially in the white matter bundles of the entire central nervous system of adult mice with less marked expression inneuronal cell bodies. The enzyme co-localizes with myelin basic protein in myelin sheaths but not in axons 707934 1.1.1.188 brain high activity 687797 1.1.1.188 brain highest activity 687797 1.1.1.188 brain low expression level 760437 1.1.1.188 breast normal breast expresses very high levels of AKR1C3 relative to other tissues 697271 1.1.1.188 breast cancer cell 18fold increase in the median AKR1C3 mRNA levels in breast cancer patients as compared to those without disease 697271 1.1.1.188 breast cancer cell of invasive ductal carcinoma of the breast, immunohistochemical analysis 740999 1.1.1.188 cardiomyocyte - 725083 1.1.1.188 CCD1113Sk cell - 740414 1.1.1.188 cecum low activity 687797 1.1.1.188 cerebral cortex - 389457 1.1.1.188 choriodecidua production of PGE2, PGF2 and PGD2 and high prostaglandin turnover 713047 1.1.1.188 conceptus peri- and postimplantation conceptus. Prostaglandin H synthase and prostaglandin E synthase are down-regulated, and prostaglandin G synthase, prostaglandin F synthase and carbonyl reductase/prostaglandin 9-reductase are down-regulated in conceptuses during trophoblasic elongation. After initiation of implantation, expression of prostaglandin G synthase, prostaglandin F synthase and carbonyl reductase/prostaglandin 9-reductase in conceptuses increases and remains higher until days 24-25 of pregnancy 688008 1.1.1.188 corpus luteum at days 12-14 of pregnancy and the estrous cycle, the expression of prostaglandin E2 synthase, prostaglandin F synthase, and carbonyl reductase/prostaglandin 9-ketoreductase genes and prostaglandin E2 synthase/prostaglandin F synthase ratio are significantly higher in corpora lutea of the pregnant gilts compared to the corpora lutea from the parallel ovaries of the cyclic gilts. There is no difference in expression of prostaglandin E2 synthase, prostaglandin F synthase, and carbonyl reductase/prostaglandin 9-ketoreductase genes and prostaglandin E2 synthase/prostaglandin F synthase ratio between corpora lutea ipsi- and contralateral to the uterine horn with the developing embryos 689092 1.1.1.188 corpus luteum high expression level of the lung-type and the microsomal endometrium-type isozymes 661605 1.1.1.188 corpus luteum no or very low expression of prostaglandin F2alpha synthase synthase 684436 1.1.1.188 DU-145 cell - 741088 1.1.1.188 embryo - 661605 1.1.1.188 endometrial stromal cell isoform AKR1B1 is expressed at a high level during the menstrual cycle during the secretory phase and in both epithelial and stromal cells 725608 1.1.1.188 endometrium - 670525 1.1.1.188 endometrium during luteolysis, activity of 20alpha-hydroxysteroid dehydrogenase synthesizing prostaglandin F2alpha 667422 1.1.1.188 endometrium from cyclic and pregnant animals, high expression level of the microsomal endometrium-type isozyme, spatiotemporal expression during estrous cycle and early pregnancy 661605 1.1.1.188 endothelium - 725083 1.1.1.188 endothelium isozyme PGF synthase I in endothelial cells of the whole area of the spinal cord, and isozyme PGF synthase II in endothelial cells of the blood vessels only 655139 1.1.1.188 endothelium liver-type isozyme, sinusoidal endothelial cells in the liver 654159 1.1.1.188 ependymal cell isozyme PGF synthase II 655139 1.1.1.188 epimastigote - -, 761141 1.1.1.188 epithelium isoform AKR1B1 is expressed at a high level during the menstrual cycle during the secretory phase and in both epithelial and stromal cells 725608 1.1.1.188 eye - 687797 1.1.1.188 eye anterior uveal and scleral complex 389459 1.1.1.188 eye low activity 687797 1.1.1.188 fetal membrane - 740487 1.1.1.188 fibroblast - 740414 1.1.1.188 GOTO cell glioblastoma cell 654851 1.1.1.188 granulosa cell both activities of enzymes AKR1C1/AKR1C2 and AKR1C3 are present in the periovuatory granulosa cell -, 690009 1.1.1.188 granulosa cell both activities of enzymes AKR1C1/AKR1C2 and AKR1C3 are present in the periovuatory granulosa cell. Prostaglandin F synthase mRNA and protein levels are low before treatment with gonadotropin and peak 24-36 h after gonadotropin administration -, 690009 1.1.1.188 heart - 389464, 654851, 661605, 687797 1.1.1.188 heart high activity 687797 1.1.1.188 HEK-293 cell embryonic kidney cell, isozyme PGFS-I 654851 1.1.1.188 HeLa cell - 654851 1.1.1.188 intestine - 654851 1.1.1.188 KEL-FIB cell keloid fibroblasts 740414 1.1.1.188 keloid - 740414 1.1.1.188 kidney - 654851, 661605 1.1.1.188 kidney AKR1B3 704349 1.1.1.188 kidney low activity 687797 1.1.1.188 kidney proximal tubule, the enzyme is associated with the cyclooxygenase-I, COX-I 667423 1.1.1.188 large intestine low activity 687797 1.1.1.188 lens - 389458 1.1.1.188 Leydig cell co-localization of cyclooxygenase-1, prostaglandin F receptor and prostaglandin F synthase 687013 1.1.1.188 liver - 389449, 389453, 654851, 657211, 699917 1.1.1.188 liver high expression level of the lung-type and the liver-type isozymes 661605 1.1.1.188 liver liver-type isozyme 389454, 657211 1.1.1.188 liver liver-type isozyme, sinusoidal endothelial cells 654159 1.1.1.188 liver low activity 687797 1.1.1.188 liver PGFS-II, liver-type isozyme 667422 1.1.1.188 liver two different active sites 389454 1.1.1.188 lung - 389444, 389448, 389449, 389453, 389458, 389464, 654740, 654851, 657211, 661605, 667713, 687797, 704349 1.1.1.188 lung contractile interstitial cells 389451 1.1.1.188 lung low activity 687797 1.1.1.188 lung low expression level 760437 1.1.1.188 lung lung-type isozyme 389454, 654159, 657211 1.1.1.188 lung PGFS-I, lung-type isozyme 667422 1.1.1.188 lung PGH2 9-,11-endoperoxide reductase activity lung-type isozyme 657211 1.1.1.188 lung two different active sites 389452, 389455, 389456, 389460 1.1.1.188 luteal cell - -, 740353 1.1.1.188 macrophage peritoneal 654851 1.1.1.188 MCF-7 cell - 697271, 740999 1.1.1.188 MDA-MB-231 cell high expression level 740999 1.1.1.188 MDA-MB-453 cell - 740999 1.1.1.188 additional information AKR1B1 shows decreased expression in neoplastic disease, with decreased AKR1B1 immunoreactivity in carcinoma cells compared with nonneoplastic ductal cells, suggesting that AKR1B1 activity might be suppressed in tumor cells in breast cancer tissues 740999 1.1.1.188 additional information enzyme tissue localization analysis 667423 1.1.1.188 additional information immunocytochemical localization study 655139 1.1.1.188 additional information in situ enzyme immunohistochemic analysis 740487 1.1.1.188 additional information isoform AKR1C3 expression is weak or absent in luteal samples 724504 1.1.1.188 additional information isozyme expression levels and tissue distribution, overview 661605 1.1.1.188 additional information low activity in deferent duct and epididymidis 687797 1.1.1.188 additional information quantitative enzyme expression analysis during pregnancy and prepartum 740130 1.1.1.188 muscle low activity 687797 1.1.1.188 myelin enzyme co-localizes with myelin basic protein in myelin sheaths but not in axons 707934 1.1.1.188 myofibroblast - 740414 1.1.1.188 myometrium - 661605 1.1.1.188 myometrium in pregnant myometrium, PGI2, PGD2 and PGF2 synthases are highly expressed while prostaglandin dehydrogenase is underexpressed. Myometrium from non-pregnant women has lower levels of prostaglandin synthases and higher levels of prostaglandin dehydrogenase than pregnant myometrium 713047 1.1.1.188 myometrium PGH2 9-,11-endoperoxide reductase activity is expressed and active at early pregnant sows, probably also in cyclic sows 657328 1.1.1.188 neuron dendrites and somata, isozyme PGF synthase I 655139 1.1.1.188 oligodendrocyte cultured oligodendrocytes at 7 days in vitro express the enzyme in cytoplasmic processes where the enzyme is co-localized with myelin basic protein 707934 1.1.1.188 ovarian follicle - -, 690009 1.1.1.188 ovary - -, 690009 1.1.1.188 ovary abundant expression 760437 1.1.1.188 ovary high activity 687797 1.1.1.188 oviduct - 661605, 762365 1.1.1.188 pancreas - 654851 1.1.1.188 placenta - 704349, 740487 1.1.1.188 placenta expression levels in uninucleated trophoblasts of the placentome of pregnant cows, especially chorionic plate and apical tertiary villi, overview 670525 1.1.1.188 placenta the prostaglandin synthase expression profile favours production of PGD2, PGE2 and PGF2, with high levels of prostaglandin transporters and catabolic prostaglandin dehydrogenase suggesting rapid prostaglandin turnover 713047 1.1.1.188 placenta utero/placental expression of isoform AKR1C3 is found in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation 724504 1.1.1.188 promastigote promastigotes synthesize prostaglandin F2alpha in the presence of arachidonic acid, with peak production in the stationary growth phase under heat stress -, 762092 1.1.1.188 prostate - 725083 1.1.1.188 prostate cancer cell - 741088 1.1.1.188 prostate gland low expression level 760437 1.1.1.188 renal cell carcinoma cell the enzyme is associated with the cyclooxygenase-II, COX-II 667423 1.1.1.188 seminal vesicle - 657211 1.1.1.188 SK-BR-3 cell - 740999 1.1.1.188 skin - 740413 1.1.1.188 skin AKR1C3 is overexpressed in the fibrotic area of keloids (scarfs) while relatively weak staining is detected in normal skin 740414 1.1.1.188 small intestine low activity 687797 1.1.1.188 small intestine low expression level 760437 1.1.1.188 spinal cord - 687797 1.1.1.188 spinal cord colocalization of prostaglandin F2alpha receptor FP and PGFS I 705002 1.1.1.188 spinal cord isozymes PGF synthase I and II 655139 1.1.1.188 spinal cord neuronal dendrites and somata of gray matter 389445 1.1.1.188 spleen - 654851 1.1.1.188 spleen low activity 687797 1.1.1.188 squamous cell carcinoma cell AKR1C3 is overexpressed in skin squamous cell carcinoma (SCC) and affects SCC growth via prostaglandin metabolism 740413 1.1.1.188 stomach - 389464, 654851 1.1.1.188 stomach low activity 687797 1.1.1.188 SUM-185PE cell high expression level 740999 1.1.1.188 T-47D cell - 740999 1.1.1.188 tanycyte isozyme PGF synthase II 655139 1.1.1.188 testis - 654851 1.1.1.188 testis abundant expression 760437 1.1.1.188 testis expression of both cyclooxygenase-1 and prostaglandin F synthase proteins in wild-type and prostaglandin F-receptor deficient strain 687013 1.1.1.188 testis low activity 687797 1.1.1.188 thymus - 654851 1.1.1.188 thymus high activity 687797 1.1.1.188 trophoblast - 740130 1.1.1.188 trophoblast uninucleated cell 670525 1.1.1.188 U-251 cell glioblastoma cell 654851 1.1.1.188 uterine endometrium - -, 389447, 725083, 740353, 741012, 741357 1.1.1.188 uterine endometrium at oestrus and mid and late phases of pseudopregnancy 726469 1.1.1.188 uterine endometrium epithelial 389450 1.1.1.188 uterine endometrium the bovine endometrium produces huge amounts of PGF2alpha 741357 1.1.1.188 uterus endometrium of repeat-breeding cows 760315 1.1.1.188 uterus high activity 687797 1.1.1.188 uterus PGFS mRNA and protein expression in the endometrium does not differ between pregnancy and oestrous cycle but PGFS mRNA in the myometrium increases during pregnancy both in the gravid and the non-gravid uterine horns 706695 1.1.1.188 uterus the prostaglandin F synthase AKR1B1 is upregulated while prostaglandin transporter is downregulated during term labor. The amnion is more responsive than choriodecidua to stimulation with proinflammatory cytokines. The mechanisms of term and preterm labor are different, overview 740487 1.1.1.188 uterus uterine expression of isoforms AKR1C3 is up-regulated postimplantation and declines prepartum. Enzyme co-localizes with 15-prostaglandin dehydrogenase 724504 1.1.1.188 uterus uterine tissues at delivery 713047 1.1.1.188 uterus utero/placental expression of PGFS (AKR1C3) is identified in the superficial uterine glands throughout gestation and in the trophoblast cells within the feto-maternal contact zone during placentation. Colocalization of 15-hydroxyprostaglandin dehydrogenase (HPGD) with PGFS (AKR1C3) expression. Uterine PGFS (AKR1C3) is up-regulated postimplantation and declined prepartum 740130 1.1.1.188 vascular smooth muscle cell - 725083 1.1.1.188 vesicular gland low activity 687797 1.1.1.188 WI-38 cell lung fibroblast cell 654851