3.4.22.48 recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by treatment with 6 M guanidinium hydrochloride, and dilution in buffer, at pH 6.0, with 40% glycerol content or with arginine and Tween 20 supplementation, method optimization 667487 3.4.22.48 using 50 mM sodium phosphate, 150 mM sodium chloride, 1 mM EDTA, 0.5 M arginine (pH 6.0), 30% (v/v) glycerol, 1 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate 731911