5.1.3.37 epimerase activity of AlgE4 preincubated with 1 mM Na2EDTA can be restored by the addition of a molar excess of Ca2+ 734129 5.1.3.37 recombinant His-tagged catalytic domain, MEP13-C5, from Escherichia coli strain BL21 CodonPlus(DE3)RIPL inclusion bodies, the protein is successfully refolded using an on-column refolding procedure 747915