4.1.3.43	33.3	-	4-hydroxy-2-oxohexanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	46.1	-	4-hydroxy-2-oxopentanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	7.8	-	4-hydroxy-2-oxopentanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	10.6	-	4-hydroxy-2-oxohexanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.45	-	(4S)-4-hydroxy-2-oxopentanoate	H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.34	-	(4S)-4-hydroxy-2-oxopentanoate	H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	additional information	-	additional information	highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides	721615	
4.1.3.43	0.00047	-	pentaldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0005	-	Isobutyraldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0022	-	Butyraldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377