3.4.22.69 0.012 - Dabcyl-KTSAVLQSGFRKME-EDANS mutant enzyme N28A, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C 717244 3.4.22.69 0.06 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide His-tagged artificial polyprotein (cyan fluorescent protein-SARS-CoV 3CLpro-yellow fluorescent protein) 709130 3.4.22.69 0.11 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide His-tagged artificial polyprotein (SARS-CoV 3CLpro-yellow fluorescent protein) 709130 3.4.22.69 0.13 - Abz-LQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.31 - Abz-SAVLQSGK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 0.53 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide SARS-CoV 3CLpro 709130 3.4.22.69 0.643 - Dabcyl-KTSAVLQSGFRKME-EDANS wild type enzyme, in 10 mM sodium phosphate, 10 mM sodium chloride, 1 mM EDTA, 1 mM TCEP, pH 7.4, at 25°C 717244 3.4.22.69 0.877 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide E166A mutant protein 707753 3.4.22.69 1.2 - Abz-VLQSGFRK(Dnp)NH2 in the absence of sodium sulfate, pH 7.5 in 50 mM Tris-HCl buffer with 1 mM dithiothreitol, at 25°C 717308 3.4.22.69 2.83 - Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide wild-type protein 707753