4.1.3.43	0.00047	-	pentaldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0005	-	Isobutyraldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0022	-	Butyraldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0037	-	glycolaldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0132	-	propionaldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	0.0134	-	acetaldehyde	kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C	702377	
4.1.3.43	additional information	-	additional information	value not detectable for R16A variant of BphI	714244	
4.1.3.43	0.00001	-	hexaldehyde	L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00001	-	hexaldehyde	wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0001	-	hexaldehyde	L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00013	-	hexaldehyde	L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00033	-	pentaldehyde	L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00042	-	acetaldehyde	L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00047	-	pentaldehyde	wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00049	-	acetaldehyde	L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00079	-	Butyraldehyde	L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00104	-	Butyraldehyde	L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0022	-	Butyraldehyde	wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0038	-	pentaldehyde	L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.00673	-	propionaldehyde	L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0117	-	acetaldehyde	L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.012	-	(4S)-4-hydroxy-2-oxopentanoate	R16K variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.0129	-	Butyraldehyde	L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0132	-	propionaldehyde	wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0134	-	acetaldehyde	wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0156	-	propionaldehyde	L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0159	-	pyruvate	L87A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.0175	-	propionaldehyde	L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0233	-	pentaldehyde	L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C	714244	
4.1.3.43	0.0587	-	pyruvate	L89A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.0915	-	pyruvate	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	714244, 722458	
4.1.3.43	0.34	-	(4S)-4-hydroxy-2-oxopentanoate	H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	0.45	-	(4S)-4-hydroxy-2-oxopentanoate	H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	10.4	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	12.6	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	13.4	-	(4S)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	14.1	-	(4R)-4-hydroxy-2-oxopentanoate	Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	44.7	-	(4S)-4-hydroxy-2-oxopentanoate	wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C	714244	
4.1.3.43	additional information	-	additional information	highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides	721615	
4.1.3.43	1.8	-	4-hydroxy-2-oxoheptanoate	pH 8.0, 25°C	721615	
4.1.3.43	2.2	-	oxaloacetate	pH 8.0, 25°C	721615	
4.1.3.43	18.6	-	4-hydroxy-2-oxopentanoate	pH 8.0, 25°C	721615	
4.1.3.43	21.1	-	4-hydroxy-2-oxohexanoate	pH 8.0, 25°C	721615	
4.1.3.43	0.8	-	4-hydroxy-2-oxopentanoate	with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	1.6	-	4-hydroxy-2-oxopentanoate	with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	4.9	-	4-hydroxy-2-oxopentanoate	single TTHB246, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	5.2	-	4-hydroxy-2-oxohexanoate	single TTHB246, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	7.8	-	4-hydroxy-2-oxopentanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	9.3	-	4-hydroxy-2-oxopentanoate	TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	10.1	-	4-hydroxy-2-oxohexanoate	TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	10.6	-	4-hydroxy-2-oxohexanoate	BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	11	-	4-hydroxy-2-oxopentanoate	with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	13	-	4-hydroxy-2-oxopentanoate	with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	13.5	-	4-hydroxy-2-oxohexanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	14.6	-	4-hydroxy-2-oxopentanoate	TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	19	-	4-hydroxy-2-oxopentanoate	with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C	721659	
4.1.3.43	33.3	-	4-hydroxy-2-oxohexanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	46.1	-	4-hydroxy-2-oxopentanoate	BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C	721659	
4.1.3.43	0.00004	-	pentaldehyde	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.000051	-	pentaldehyde	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.00016	-	Butyraldehyde	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.00031	-	Butyraldehyde	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.00047	-	pentaldehyde	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0015	-	Butyraldehyde	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0017	-	pentaldehyde	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0018	-	propionaldehyde	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.002	-	propionaldehyde	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.00219	-	Butyraldehyde	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0025	-	acetaldehyde	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0036	-	pyruvate	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0043	-	acetaldehyde	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0043	-	pyruvate	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0057	-	propionaldehyde	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0058	-	oxaloacetate	L87W variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0069	-	acetaldehyde	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0084	-	pyruvate	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0132	-	propionaldehyde	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.0134	-	acetaldehyde	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.064	-	oxaloacetate	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.098	-	oxaloacetate	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.1	-	(4R)-4-hydroxy-2-oxopentanoate	L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.13	-	(4R)-4-hydroxy-2-oxopentanoate	L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.254	-	oxaloacetate	L87N variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	0.53	-	oxaloacetate	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	2.2	-	oxaloacetate	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	10.4	-	(4R)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	12.6	-	(4S)-4-hydroxy-2-oxopentanoate	Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458	
4.1.3.43	44.7	-	(4S)-4-hydroxy-2-oxopentanoate	wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C	722458