3.5.1.19 0.0086 - 1-methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.0096 - nicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.025 - Benzamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.05 - ethyl nicotinate recombinant mutant Q96K, pH 7.3, 37°C 735084 3.5.1.19 0.05 - Thionicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.061 - 5-Methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.1 - ethyl nicotinate recombinant wild-type enzyme, pH 7.3, 37°C 735084 3.5.1.19 0.1 - ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F 755150 3.5.1.19 0.13 - ethyl nicotinate recombinant mutant Q96A, pH 7.3, 37°C 735084 3.5.1.19 0.157 - Pyrazinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.2 - ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E 755150 3.5.1.19 0.2 - ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S 755150 3.5.1.19 0.3 - ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C 755150 3.5.1.19 0.31 - methyl nicotinate recombinant wild-type enzyme, pH 7.3, 37°C 735084 3.5.1.19 0.35 - Benzamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 0.38 - methyl nicotinate recombinant mutant Q96K, pH 7.3, 37°C 735084 3.5.1.19 0.39 - Pyrazinamide recombinant mutant Q96K, pH 7.3, 37°C 735084 3.5.1.19 0.41 - ethyl nicotinate recombinant mutant C133A, pH 7.3, 37°C 735084 3.5.1.19 0.52 - Pyrazinamide recombinant mutant E65H, pH 7.3, 37°C 735084 3.5.1.19 1 - ethylnicotinate pH 7.3, temperature not specified in the publication 753679 3.5.1.19 1.1 - Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F 755150 3.5.1.19 1.14 - methyl nicotinate recombinant mutant C133A, pH 7.3, 37°C 735084 3.5.1.19 1.17 - methyl nicotinate recombinant mutant Q96A, pH 7.3, 37°C 735084 3.5.1.19 1.34 - Pyrazinamide recombinant mutant Q96A, pH 7.3, 37°C 735084 3.5.1.19 1.4 - methylnicotinate 37°C, pH 7.3, mutant enzyme W83F 755150 3.5.1.19 2.2 - nicotinamide mutant enzyme H53A, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 2.2 - Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S 755150 3.5.1.19 2.8 - ethylnicotinate 37°C, pH 7.3, wild-type enzyme 755150 3.5.1.19 2.8 - methylnicotinate 37°C, pH 7.3, mutant enzyme H75S 755150 3.5.1.19 3.2 - Pyrazinamide recombinant wild-type enzyme, pH 7.3, 37°C 735084 3.5.1.19 3.4 - 5-methyl nicotinamide recombinant mutant Q96K, pH 7.3, 37°C 735084 3.5.1.19 4.15 - nicotinamide recombinant mutant Q96K, pH 7.3, 37°C 735084 3.5.1.19 5.2 - Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E 755150 3.5.1.19 6.2 - Pyrazinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C 718885 3.5.1.19 6.28 - Pyrazinamide recombinant mutant F68W/C133A, pH 7.3, 37°C 735084 3.5.1.19 6.7 - nicotinamide kcat/Km above 6.7 (1/sec*mM), mutant enzyme K122A, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 7.4 - nicotinamide kcat/Km above 7.4 (1/sec*mM), mutant enzyme H94A, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 7.5 - 5-methyl nicotinamide recombinant wild-type enzyme, pH 7.3, 37°C 735084 3.5.1.19 7.9 - Pyrazinamide recombinant mutant F68W, pH 7.3, 37°C 735084 3.5.1.19 8.3 - nicotinamide kcat/Km above 8.3 (1/sec*mM), mutant enzyme D51A, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 9.19 - Pyrazinamide recombinant mutant C133A, pH 7.3, 37°C 735084 3.5.1.19 11.07 - 5-methyl nicotinamide recombinant mutant C133A, pH 7.3, 37°C 735084 3.5.1.19 13 - Pyrazinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C 718885 3.5.1.19 14.1 - methylnicotinate 37°C, pH 7.3, mutant enzyme H75E 755150 3.5.1.19 16 - Pyrazinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 18.5 - 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F 755150 3.5.1.19 18.9 - 5-Methylnicotinamide pH 7.3, temperature not specified in the publication 753679 3.5.1.19 19.7 - methylnicotinate pH 7.3, temperature not specified in the publication 753679 3.5.1.19 20.5 - methylnicotinate 37°C, pH 7.3, mutant enzyme A151C 755150 3.5.1.19 29 - 5-Methylnicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 29.04 - 5-methyl nicotinamide recombinant mutant Q96A, pH 7.3, 37°C 735084 3.5.1.19 34 - nicotinamide recombinant mutant C133A, pH 7.3, 37°C 735084 3.5.1.19 34.1 - Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C 755150 3.5.1.19 35 - 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E 755150 3.5.1.19 35 - nicotinamide kcat/Km above 35 (1/sec*mM), mutant enzyme D51N, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 37 - nicotinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C 718885 3.5.1.19 39.7 - nicotinamide 37°C, pH 7.3, mutant enzyme W83F 755150 3.5.1.19 40.1 - 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme 755150 3.5.1.19 42.65 - nicotinamide recombinant mutant E65H, pH 7.3, 37°C 735084 3.5.1.19 43.48 - nicotinamide recombinant wild-type enzyme, pH 7.3, 37°C 735084 3.5.1.19 44.59 - nicotinamide recombinant mutant Q96A, pH 7.3, 37°C 735084 3.5.1.19 45.7 - 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C 755150 3.5.1.19 50.5 - methylnicotinate 37°C, pH 7.3, wild-type enzyme 755150 3.5.1.19 51 - nicotinamide 37°C, pH 7.3, mutant enzyme A151C 755150 3.5.1.19 52.01 - nicotinamide recombinant mutant F68W, pH 7.3, 37°C 735084 3.5.1.19 52.8 - nicotinamide recombinant mutant F68W/C133A, pH 7.3, 37°C 735084 3.5.1.19 53.9 - 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S 755150 3.5.1.19 54.3 - nicotinamide 37°C, pH 7.3, mutant enzyme H75S 755150 3.5.1.19 56.7 - Pyrazinamide 37°C, pH 7.3, wild-type enzyme 755150 3.5.1.19 68 - nicotinamide 37°C, pH 7.3, mutant enzyme H75E 755150 3.5.1.19 69.9 - Pyrazinamide pH 7.3, temperature not specified in the publication 753679 3.5.1.19 72 - nicotinamide wild type enzyme, in 50 mM sodium phosphate (pH 7.5), at 25°C 718924 3.5.1.19 102 - nicotinamide pH 7.3, temperature not specified in the publication 753679 3.5.1.19 189.2 - nicotinamide 37°C, pH 7.3, wild-type enzyme 755150 3.5.1.19 220 - nicotinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C 718885 3.5.1.19 331 - Pyrazinamide pH 7.5, 60°C 729184 3.5.1.19 427 - nicotinamide pH 7.5, 60°C 729184 3.5.1.19 427 - nicotinamide pH and temperature not specified in the publication 727053 3.5.1.19 3000 - nicotinamide pH and temperature not specified in the publication 754017