1.1.1.203 additional information - additional information Michaelis-Menten kinetics and thermodynamics 740393 1.1.1.203 0.03 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.03 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.04 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.04 - L-gulono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 0.05 - L-galactono-1,4-lactone with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 0.07 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.1 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 0.11 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.2 - L-Gulonic acid with NADP+ as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 0.23 - L-galactonate with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 0.3 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.32 - D-glucurono-3,6-lactone with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 0.7 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 0.7 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 1.2 - D-glucuronic acid with NADH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 3.89 - D-glucuronic acid with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 4 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 5.87 - D-galacturonic acid with NADH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 9 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 11 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 11 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 12 - D-Mannuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 14.3 - D-galacturonic acid with NADPH as cosubstrate, at pH 7.5 and 25°C 761270 1.1.1.203 19 - NADH at pH 7.5 and 25°C 761270 1.1.1.203 41 - beta-D-galacturonate mutant enzyme I114T, at pH 8.0 and 25°C 762310 1.1.1.203 48 - beta-D-glucuronate mutant enzyme I114T, at pH 8.0 and 25°C 762310 1.1.1.203 77 - beta-D-glucuronate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 78 - beta-D-galacturonate mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 88 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 93.5 - beta-D-glucuronate pH 8.0, 25°C, method B 740393 1.1.1.203 93.7 - beta-D-glucuronate pH 8.0, 25°C 740393 1.1.1.203 96 - beta-D-galacturonate pH 8.0, 25°C 740393 1.1.1.203 97.5 - beta-D-glucuronate mutant enzyme H236K, at pH 8.0 and 25°C 760328 1.1.1.203 98 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 101 - beta-D-glucuronate mutant enzyme Y116F, at pH 8.0 and 25°C 762310 1.1.1.203 104 - beta-D-galacturonate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C 762310 1.1.1.203 109 - beta-D-glucuronate pH 8.0, 25°C, method B 740393 1.1.1.203 111 - beta-D-galacturonate mutant enzyme Y116F, at pH 8.0 and 25°C 762310 1.1.1.203 111 - beta-D-glucuronate mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C 762310 1.1.1.203 112 - beta-D-galacturonate mutant enzyme I88L, at pH 8.0 and 25°C 762310 1.1.1.203 115 - beta-D-glucuronate mutant enzyme T113V, at pH 8.0 and 25°C 762310 1.1.1.203 116 - beta-D-galacturonate mutant enzyme M175I, at pH 8.0 and 25°C 762310 1.1.1.203 116.7 - beta-D-glucuronate mutant enzyme H101Y/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 125 - beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C 762310 1.1.1.203 125 - beta-D-galacturonate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 125 - beta-D-glucuronate mutant enzyme I88L, at pH 8.0 and 25°C 762310 1.1.1.203 126 - beta-D-galacturonate mutant enzyme D246N, at pH 8.0 and 25°C 762310 1.1.1.203 126 - beta-D-galacturonate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 130 - beta-D-galacturonate pH 8.0, 25°C, method B 740393 1.1.1.203 132 - beta-D-galacturonate mutant enzyme T113V, at pH 8.0 and 25°C 762310 1.1.1.203 136 - beta-D-glucuronate mutant enzyme A172V, at pH 8.0 and 25°C 762310 1.1.1.203 136 - beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 138 - beta-D-galacturonate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 138 - beta-D-glucuronate mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C 762310 1.1.1.203 139 - beta-D-glucuronate mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 139.7 - beta-D-glucuronate mutant enzyme H101N/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 146 - beta-D-glucuronate mutant enzyme D246N, at pH 8.0 and 25°C 762310 1.1.1.203 149 - beta-D-glucuronate mutant enzyme M175I, at pH 8.0 and 25°C 762310 1.1.1.203 149.7 - beta-D-glucuronate mutant enzyme A41P/H101N/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 151 - beta-D-galacturonate mutant enzyme A172V, at pH 8.0 and 25°C 762310 1.1.1.203 152 - beta-D-galacturonate mutant enzyme Q55R, at pH 8.0 and 25°C 762310 1.1.1.203 155 - beta-D-galacturonate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 160.5 - beta-D-glucuronate mutant enzyme A41P/H101Y/H236R, at pH 8.0 and 25°C 760328 1.1.1.203 161 - beta-D-galacturonate pH 8.0, 25°C 740393 1.1.1.203 161 - beta-D-glucuronate mutant enzyme Q55R, at pH 8.0 and 25°C 762310 1.1.1.203 161 - beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C 762310 1.1.1.203 162 - beta-D-glucuronate mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C 762310 1.1.1.203 162 - beta-D-glucuronate pH 8.0, 25°C 740393 1.1.1.203 164 - beta-D-glucuronate mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C 762310 1.1.1.203 167.3 - beta-D-glucuronate mutant enzyme A41P/H101Y/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 170 - beta-D-glucuronate mutant enzyme A41P/H101N/H236R, at pH 8.0 and 25°C 760328 1.1.1.203 172 - beta-D-galacturonate wild type enzyme, at pH 8.0 and 25°C 762310 1.1.1.203 179.5 - beta-D-glucuronate mutant enzyme A41P/H236K, at pH 8.0 and 25°C 760328 1.1.1.203 185 - beta-D-galacturonate pH 8.0, 25°C, method B 740393 1.1.1.203 186 - beta-D-glucuronate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C 762310 1.1.1.203 187 - beta-D-galacturonate mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C 762310 1.1.1.203 200 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 201 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 210 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 211 - beta-D-glucuronate pH 8.0, 25°C, method A 740393 1.1.1.203 220 - beta-D-glucuronate pH 8.0, 25°C 740393 1.1.1.203 228 - beta-D-galacturonate pH 8.0, 25°C, method A 740393 1.1.1.203 257 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 260 - beta-D-galacturonate pH 8.0, 25°C 740393 1.1.1.203 269 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 274 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 286.4 - beta-D-glucuronate wild type enzyme, at pH 8.0 and 25°C 760328 1.1.1.203 328 - beta-D-galacturonate pH 8.0, 25°C, method A 740393 1.1.1.203 331 - beta-D-glucuronate pH 8.0, 25°C, method A 740393 1.1.1.203 342 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 400.014 - D-glucuronic acid from a beechwood xylan at pH 7.0 and 60°C 762511 1.1.1.203 406 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 430 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 465 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 509.018 - D-galacturonic acid at pH 7.0 and 60°C 762511 1.1.1.203 622.22 - NAD+ at pH 7.0 and 60°C 762511 1.1.1.203 628 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 638 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 646 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 720 - D-glucuronic acid mutant enzyme A39P/H99Y, at pH 8.0 and 30°C 760723 1.1.1.203 730 - D-glucuronic acid mutant enzyme A39P, at pH 8.0 and 30°C 760723 1.1.1.203 770 - D-glucuronic acid mutant enzyme H99Y, at pH 8.0 and 30°C 760723 1.1.1.203 785 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 794 - beta-D-galacturonate recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 800 - D-glucuronic acid wild type enzyme, at pH 8.0 and 30°C 760723 1.1.1.203 810 - D-glucuronic acid mutant enzyme H234K, at pH 8.0 and 30°C 760723 1.1.1.203 829 - beta-D-glucuronate recombinant His-tagged enzyme, pH and temperature not specified in the publication, Tris buffer 740959 1.1.1.203 840 - D-glucuronic acid mutant enzyme A39P/H234K, at pH 8.0 and 30°C 760723 1.1.1.203 860 - D-glucuronic acid mutant enzyme H99Y/H234K, at pH 8.0 and 30°C 760723 1.1.1.203 910 - D-glucuronic acid mutant enzyme A39P/H99Y/H234K, at pH 8.0 and 30°C 760723 1.1.1.203 1083 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 1262 - NAD+ recombinant His-tagged enzyme, pH and temperature not specified in the publication, potassium phosphate buffer 740959 1.1.1.203 1655 - NADPH with D-glucuronic acid as cosubstrate, at pH 7.5 and 25°C 761270