4.1.3.43 additional information - additional information highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides 721615 4.1.3.43 additional information - additional information value not detectable for R16A variant of BphI 714244 4.1.3.43 0.00001 - hexaldehyde L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C 714244 4.1.3.43 0.00001 - hexaldehyde wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C 714244 4.1.3.43 0.00004 - pentaldehyde L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C 722458 4.1.3.43 0.000051 - pentaldehyde L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C 722458 4.1.3.43 0.0001 - hexaldehyde L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C 714244 4.1.3.43 0.00013 - hexaldehyde L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C 714244 4.1.3.43 0.00016 - Butyraldehyde L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C 722458 4.1.3.43 0.00031 - Butyraldehyde L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C 722458