5.4.99.59 malfunction mutant strains H1863 lacking fcf2. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf2 is required for the synthesis of the O antigen in Escherichia coli O52 -, 723209 5.4.99.59 physiological function the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O antigen -, 723209